Gli1 Antibody - #DF7523

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产品: Gli1 抗体
货号: DF7523
描述: Rabbit polyclonal antibody to Gli1
应用: WB IHC IF/ICC
反应: Human, Mouse, Rat
分子量: 118 kDa; 118kD(Calculated).
蛋白号: P08151
RRID: AB_2841022

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产品描述

来源:
Rabbit
应用:
WB 1:1000-3000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
克隆:
Polyclonal
特异性:
Gli1 Antibody detects endogenous levels of total Gli1.
RRID:
AB_2841022
引用格式: Affinity Biosciences Cat# DF7523, RRID:AB_2841022.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Gli 1; GLI; GLI family zinc finger 1; GLI Kruppel family member 1; gli1; GLI1_HUMAN; Glioma associated oncogene 1; Glioma associated oncogene homolog 1 (zinc finger protein); Glioma associated oncogene homolog; Glioma-associated oncogene; Oncogene GLI; Zfp 5; Zfp5; Zinc finger protein GLI 1; Zinc finger protein GLI1;

抗原和靶标

免疫原:
Uniprot:

P08151(GLI1_HUMAN) >>Visit HPA database.

基因/基因ID:
GLI1(2735)
表达:
P08151 GLI1_HUMAN:

Detected in testis (at protein level) (PubMed:2105456). Testis, myometrium and fallopian tube. Also expressed in the brain with highest expression in the cerebellum, optic nerve and olfactory tract (PubMed:19878745). Isoform 1 is detected in brain, spleen, pancreas, liver, kidney and placenta; isoform 2 is not detectable in these tissues (PubMed:19706761).

序列:
MFNSMTPPPISSYGEPCCLRPLPSQGAPSVGTEGLSGPPFCHQANLMSGPHSYGPARETNSCTEGPLFSSPRSAVKLTKKRALSISPLSDASLDLQTVIRTSPSSLVAFINSRCTSPGGSYGHLSIGTMSPSLGFPAQMNHQKGPSPSFGVQPCGPHDSARGGMIPHPQSRGPFPTCQLKSELDMLVGKCREEPLEGDMSSPNSTGIQDPLLGMLDGREDLEREEKREPESVYETDCRWDGCSQEFDSQEQLVHHINSEHIHGERKEFVCHWGGCSRELRPFKAQYMLVVHMRRHTGEKPHKCTFEGCRKSYSRLENLKTHLRSHTGEKPYMCEHEGCSKAFSNASDRAKHQNRTHSNEKPYVCKLPGCTKRYTDPSSLRKHVKTVHGPDAHVTKRHRGDGPLPRAPSISTVEPKREREGGPIREESRLTVPEGAMKPQPSPGAQSSCSSDHSPAGSAANTDSGVEMTGNAGGSTEDLSSLDEGPCIAGTGLSTLRRLENLRLDQLHQLRPIGTRGLKLPSLSHTGTTVSRRVGPPVSLERRSSSSSSISSAYTVSRRSSLASPFPPGSPPENGASSLPGLMPAQHYLLRARYASARGGGTSPTAASSLDRIGGLPMPPWRSRAEYPGYNPNAGVTRRASDPAQAADRPAPARVQRFKSLGCVHTPPTVAGGGQNFDPYLPTSVYSPQPPSITENAAMDARGLQEEPEVGTSMVGSGLNPYMDFPPTDTLGYGGPEGAAAEPYGARGPGSLPLGPGPPTNYGPNPCPQQASYPDPTQETWGEFPSHSGLYPGPKALGGTYSQCPRLEHYGQVQVKPEQGCPVGSDSTGLAPCLNAHPSEGPPHPQPLFSHYPQPSPPQYLQSGPYTQPPPDYLPSEPRPCLDFDSPTHSTGQLKAQLVCNYVQSQQELLWEGGGREDAPAQEPSYQSPKFLGGSQVSPSRAKAPVNTYGPGFGPNLPNHKSGSYPTPSPCHENFVVGANRASHRAAAPPRLLPPLPTCYGPLKVGGTNPSCGHPEVGRLGGGPALYPPPEGQVCNPLDSLDLDNTQLDFVAILDEPQGLSPPPSHDQRGSSGHTPPPSGPPNMAVGNMSVLLRSLPGETEFLNSSA

翻译修饰 - P08151 作为底物

Site PTM Type Enzyme
Phosphorylation
Ubiquitination
T63 Phosphorylation
S69 Phosphorylation
S84 Phosphorylation P23443 (RPS6KB1)
S102 Phosphorylation P54646 (PRKAA2)
S130 Phosphorylation
R161 Methylation
S201 Phosphorylation
S204 Phosphorylation
T205 Phosphorylation
S243 Phosphorylation P41743 (PRKCI)
T304 Phosphorylation P41743 (PRKCI)
K319 Ubiquitination
K365 Acetylation
K371 Acetylation
T374 Phosphorylation P17612 (PRKACA)
S408 Phosphorylation P54646 (PRKAA2)
K518 Acetylation
S521 Phosphorylation
T527 Phosphorylation
S538 Phosphorylation
S544 Phosphorylation P17612 (PRKACA)
S545 Phosphorylation
S560 Phosphorylation P17612 (PRKACA)
R597 Methylation
S602 Phosphorylation
S622 Phosphorylation
S640 Phosphorylation P17612 (PRKACA)
S934 Phosphorylation
S937 Phosphorylation
S939 Phosphorylation
S968 Phosphorylation
S1071 Phosphorylation
T1074 Phosphorylation P54646 (PRKAA2)
S1078 Phosphorylation

研究背景

功能:

Acts as a transcriptional activator. Binds to the DNA consensus sequence 5'-GACCACCCA-3'. Regulates the transcription of specific genes during normal development. Plays a role in craniofacial development and digital development, as well as development of the central nervous system and gastrointestinal tract. Mediates SHH signaling. Plays a role in cell proliferation and differentiation via its role in SHH signaling.

Acts as a transcriptional activator, but activates a different set of genes than isoform 1. Activates expression of CD24, unlike isoform 1. Mediates SHH signaling. Promotes cancer cell migration.

翻译修饰:

Phosphorylated in vitro by ULK3.

Acetylation at Lys-518 down-regulates transcriptional activity. Deacetylated by HDAC1.

细胞定位:

Cytoplasm. Nucleus.
Note: Tethered in the cytoplasm by binding to SUFU (PubMed:10806483). Activation and translocation to the nucleus is promoted by interaction with STK36 (PubMed:10806483). Phosphorylation by ULK3 may promote nuclear localization (PubMed:19878745). Translocation to the nucleus is promoted by interaction with ZIC1 (PubMed:11238441).

Cytoplasm. Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Detected in testis (at protein level). Testis, myometrium and fallopian tube. Also expressed in the brain with highest expression in the cerebellum, optic nerve and olfactory tract. Isoform 1 is detected in brain, spleen, pancreas, liver, kidney and placenta; isoform 2 is not detectable in these tissues.

亚基结构:

Interacts with KIF7 (By similarity). Interacts with STK36. Interacts with ZIC1; the interaction enhances transcription activation. Interacts with SUFU; this inhibits transcriptional activation by GLI1.

蛋白家族:

Belongs to the GLI C2H2-type zinc-finger protein family.

研究领域

· Environmental Information Processing > Signal transduction > cAMP signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Hedgehog signaling pathway.   (View pathway)

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Basal cell carcinoma.   (View pathway)

文献引用

1). Inoscavin A, a pyrone compound isolated from a Sanghuangporus vaninii extract, inhibits colon cancer cell growth and induces cell apoptosis via the hedgehog signaling pathway. Phytomedicine (PubMed: 35026508) [IF=7.9]
2). Hedgehog pathway inhibition causes primary follicle atresia and decreases female germline stem cell proliferation capacity or stemness. Stem Cell Research & Therapy (PubMed: 31277696) [IF=7.5]

Application: IHC    Species: mouse    Sample: ovarian

Fig. 1 | Hh signaling pathway activity decreases with ovarian aging. a mRNA expression levels of Gli1, Ptch1, and Cyclin D1 in three mice of reproductive age. b, c Protein expression levels.d IHC detection of key members of the Hh pathway, Gli1, Ptch1, and Cyclin D1, in the OSE. Bar is 10 μm. *p < 0.05 and **p < 0.01

Application: IF/ICC    Species: mouse    Sample: ovarian

Fig. 2| Ovarian aging has a similar trend as the decrease in Hh activity. a mRNA levels of Mvh decrease gradually along with ovarian aging. bOct4 mRNA levels have the same trend as Mvh levels. c, d Protein levels of Mvh and Oct4 at three reproductive age points. e, f Dual-IF showed the coexpression of Mvh and Gli1, Oct4, and Gli1 in OSE. Bar is 20 μm. *p < 0.05 and **p < 0.01

Application: WB    Species: mouse    Sample: ovarian

Fig. 3| Pathological ovaries exhibited a decline in Hh pathway activity. a HE staining of a normal ovary and POF ovary. Bar is 200 μm. b The ratio of atretic follicles of normal and POF ovaries. c IHC shows that the Mvh-positive follicles and FGSCs dramatically disappeared in the POF model;green arrows are FGSCs, and black arrow represents primordial follicles, the scale is 100 μm. d–f mRNA and protein expression levels of Mvh, Oct4, Gli1,Ptch1, and Cyclin D1. *p < 0.05 and **p < 0.01

Application: WB    Species: mouse    Sample: ovaries

Fig. |1 Hh signaling pathway activity decreases with ovarian aging. a mRNA expression levels of Gli1, Ptch1, and Cyclin D1 in three mice of reproductive age. b, c Protein expression levels.

Application: IHC    Species: mouse    Sample: ovaries

Fig. |1 Hh signaling pathway activity decreases with ovarian aging. a mRNA expression levels of Gli1, Ptch1, and Cyclin D1 in three mice of reproductive age. b, c Protein expression levels.d IHC detection of key members of the Hh pathway, Gli1, Ptch1, and Cyclin D1, in the OSE. Bar is 10 μm. *p < 0.05 and **p < 0.01

Application: IF/ICC    Species: mouse    Sample: ovaries

Fig. 2 |Ovarian aging has a similar trend as the decrease in Hh activity. a mRNA levels of Mvh decrease gradually along with ovarian aging. bOct4 mRNA levels have the same trend as Mvh levels. c, d Protein levels of Mvh and Oct4 at three reproductive age points. e, f Dual-IF showed the coexpression of Mvh and Gli1, Oct4, and Gli1 in OSE. Bar is 20 μm. *p < 0.05 and **p < 0.01
3). Resveratrol Plays a Protective Role against Premature Ovarian Failure and Prompts Female Germline Stem Cell Survival. International Journal of Molecular Sciences (PubMed: 31340581) [IF=5.6]
4). Effects of metformin on Sonic hedgehog subgroup medulloblastoma progression: In vitro and in vivo studies. Frontiers in Pharmacology (PubMed: 36278239) [IF=5.6]
5). Gankyrin activates the hedgehog signalling to drive metastasis in osteosarcoma. JOURNAL OF CELLULAR AND MOLECULAR MEDICINE (PubMed: 34089292) [IF=5.3]

Application: WB    Species: Human    Sample: U2OS cells

FIGURE 3 Gankyrin promotes invasion, migration and regulates the expression of some stemness factors by up‐regulating Gli1. A, Gankyrin knockdown in U2OS cells reduced the mRNA expression of Gli1 and Ptch1 but not of β‐catenin and AXIN2, as shown by qRT‐PCR analysis. ns, P ≥ .05; *P < .05. B, Overexpression of gankyrin in MG63 cells increased the mRNA expression of Gli1 and Ptch1 but not of β‐catenin and AXIN2. ns, P ≥ .05; **P < .01. C, Gankyrin depletion in U2OS cells diminished the protein levels of Gli1 and PTCH1, as detected by western blot analysis. D, Ectopic expression of gankyrin in MG63 cells induced the protein levels of Gli1 and PTCH1. E, The up‐regulation of stem cell markers, including CD133, OCT4, and Nanog, following gankyrin overexpression was attenuated by Gli1 shRNA in U2OS cells, as detected by Western blot analysis. F, The increase in cell migration with gankyrin overexpression was attenuated by Gli1 shRNA in U2OS cells, as shown by wound healing assays. *P < .05; **P < .01. G, The increase in cell migration with gankyrin overexpression was attenuated by Gli1 shRNA in U2OS cells, as shown by Transwell invasion assays. *P < .05
6). Hedgehog Signaling Pathway Regulates the Proliferation and Differentiation of Rat Meibomian Gland Epithelial Cells. Investigative Ophthalmology & Visual Science (PubMed: 33616621) [IF=4.4]

Application: IF/ICC    Species: Rat    Sample: epithelial cells

Figure 1. Expression of Hedgehog receptor and downstream molecules in rat meibomian glands. (A) At P3, Smo was broadly expressed in almost all epithelial cells. By 6 weeks, Smo was expressed in all acini, and by 12 weeks, the expression of Smo in acini were generally low. (B) Gli1 was expressed in the cytoplasm of meibomian glands at 3 days, 6 weeks, and 12 weeks old. (C) Gli2 was highly expressed in the cytoplasm at 3 days old, but by 6 weeks and 12 weeks, the expression was almost absent. (D) Gli3 was hardly expressed at 3 days, 6 weeks, and 12 weeks. Du, duct; Ac, acinus.
7). (‑)‑Epigallocatechin‑3‑gallate inhibits bladder cancer stem cells via suppression of sonic hedgehog pathway. ONCOLOGY REPORTS (PubMed: 31180522) [IF=4.2]

Application: WB    Species: human    Sample: Bladder CSCs

Figure 3.| - EGCG induces apoptosis and reduces the expression of cell proliferation-related proteins on bladder CSCs. Bladder CSCs were treated with different concentrations of EGCG for 7 days. (D) The expression levels of Shh signaling pathway-related proteins including Shh, Smo, Gli1 and Gli2 were detected by western blot analysis. Data are expressed as mean ± standard deviation. *P<0.05, **P<0.01 vs. the EGCG-0 µm group. EGCG, (−)-epigallocatechin-3-gallate; CSCs, cancer stem cells; PCNA, proliferating cell nuclear antigen; Shh, sonic hedgehog protein; Smo, smoothened homolog; Gli, zinc finger protein GLI.
8). Curcumin mitigates the epithelial-to-mesenchymal transition in biliary epithelial cells through upregulating CD109 expression. DRUG DEVELOPMENT RESEARCH (PubMed: 31403228) [IF=3.8]

Application: WB    Species: human    Sample: HIBECs

FIGURE 5 |Curcumin suppresses the TGF-β1-activated Hedgehog signaling in HIBECs. (a) Quantitative analysis of Shh, Gli1, Smo, Ptch-1, and Sufu protein expression.
9). Dehydroepiandrosterone alleviates E. Coli O157:H7-induced inflammation by preventing the activation of p38 MAPK and NF-κB pathways in mice peritoneal macrophages. MOLECULAR IMMUNOLOGY (PubMed: 31351412) [IF=3.6]
10). Inhibitory Effects of Rhein on Renal Interstitial Fibrosis via the SHH-Gli1 Signal Pathway. Evidence-based Complementary and Alternative Medicine (PubMed: 35966731)

Application: WB    Species: Rat    Sample:

Figure 3 Inhibitory effect of rhein on SHH signaling pathway in UUO rats. Rhein group rats were administered rhein 2 mg·kg−1·d−1 by oral gavage for 14-d sham group, and UUO group rats were administered equal volumes of water. SHH, Gli1, and Snail protein and mRNA expression levels were determined by western blotting and RT-PCR. Data are presented as mean ± SEM. ∗p < 0.05, ∗∗p < 0.01.
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