产品: MST1/MST2 抗体
货号: DF8569
描述: Rabbit polyclonal antibody to MST1/MST2
应用: WB IF/ICC
反应: Human, Mouse, Rat
预测: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
分子量: 56 kDa; 56kD(Calculated).
蛋白号: Q13043 | Q13188
RRID: AB_2841773

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产品描述

来源:
Rabbit
应用:
WB 1:1000-3000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
克隆:
Polyclonal
特异性:
MST1/MST2 Antibody detects endogenous levels of total MST1/MST2.
RRID:
AB_2841773
引用格式: Affinity Biosciences Cat# DF8569, RRID:AB_2841773.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Kinase responsive to stress; Krs2; Mammalian STE20 like protein kinase 1; Mammalian STE20-like protein kinase 1; Mammalian sterile 20 like 1; MST-1; MST1; Serine/threonine kinase 4; Serine/threonine protein kinase Krs 2; Serine/threonine-protein kinase 4; Serine/threonine-protein kinase Krs-2; STE20 like kinase MST1; STE20-like kinase MST1; STK4; STK4_HUMAN; TIIAC; YSK3; 0610042I06Rik; EC 2.7.11.1; FLJ90748; KB 1458E12.1; Kinase responsive to stress 1; KRS1; Mammalian STE20 like protein kinase 2; Mammalian STE20-like protein kinase 2; Mammalian sterile 20-like 2; Mess1; MST; MST-2; MST2; Mst3; Serine/threonine kinase 3 (STE20 homolog, yeast); Serine/threonine kinase 3 (Ste20, yeast homolog); Serine/threonine kinase 3; Serine/threonine protein kinase 3; Serine/threonine protein kinase Krs1; Serine/threonine-protein kinase 3; Serine/threonine-protein kinase Krs-1; STE20 like kinase MST2; STE20-like kinase MST2; Stk3; STK3_HUMAN; wu:fc19e11; zgc:55383;

抗原和靶标

免疫原:
Uniprot:
基因/基因ID:
表达:
Q13043 STK4_HUMAN:

Expressed in prostate cancer and levels increase from the normal to the malignant state (at protein level). Ubiquitously expressed.

Q13188 STK3_HUMAN:

Expressed at high levels in adult kidney, skeletal and placenta tissues and at very low levels in adult heart, lung and brain tissues.

序列:
METVQLRNPPRRQLKKLDEDSLTKQPEEVFDVLEKLGEGSYGSVYKAIHKETGQIVAIKQVPVESDLQEIIKEISIMQQCDSPHVVKYYGSYFKNTDLWIVMEYCGAGSVSDIIRLRNKTLTEDEIATILQSTLKGLEYLHFMRKIHRDIKAGNILLNTEGHAKLADFGVAGQLTDTMAKRNTVIGTPFWMAPEVIQEIGYNCVADIWSLGITAIEMAEGKPPYADIHPMRAIFMIPTNPPPTFRKPELWSDNFTDFVKQCLVKSPEQRATATQLLQHPFVRSAKGVSILRDLINEAMDVKLKRQESQQREVDQDDEENSEEDEMDSGTMVRAVGDEMGTVRVASTMTDGANTMIEHDDTLPSQLGTMVINAEDEEEEGTMKRRDETMQPAKPSFLEYFEQKEKENQINSFGKSVPGPLKNSSDWKIPQDGDYEFLKSWTVEDLQKRLLALDPMMEQEIEEIRQKYQSKRQPILDAIEAKKRRQQNF

MEQPPAPKSKLKKLSEDSLTKQPEEVFDVLEKLGEGSYGSVFKAIHKESGQVVAIKQVPVESDLQEIIKEISIMQQCDSPYVVKYYGSYFKNTDLWIVMEYCGAGSVSDIIRLRNKTLIEDEIATILKSTLKGLEYLHFMRKIHRDIKAGNILLNTEGHAKLADFGVAGQLTDTMAKRNTVIGTPFWMAPEVIQEIGYNCVADIWSLGITSIEMAEGKPPYADIHPMRAIFMIPTNPPPTFRKPELWSDDFTDFVKKCLVKNPEQRATATQLLQHPFIKNAKPVSILRDLITEAMEIKAKRHEEQQRELEEEEENSDEDELDSHTMVKTSVESVGTMRATSTMSEGAQTMIEHNSTMLESDLGTMVINSEDEEEEDGTMKRNATSPQVQRPSFMDYFDKQDFKNKSHENCNQNMHEPFPMSKNVFPDNWKVPQDGDFDFLKNLSLEELQMRLKALDPMMEREIEELRQRYTAKRQPILDAMDAKKRRQQNF

种属预测

种属预测:

score>80的预测可信度较高,可尝试用于WB检测。*预测模型主要基于免疫原序列比对,结果仅作参考,不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Zebrafish
100
Chicken
100
Rabbit
100
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - Q13043/Q13188 作为底物

Site PTM Type Enzyme
M1 Acetylation
K13 Ubiquitination
S15 Phosphorylation P53350 (PLK1)
S18 Phosphorylation P53350 (PLK1)
K21 Ubiquitination
K32 Ubiquitination
K43 Ubiquitination
K47 Ubiquitination
K56 Ubiquitination
Y81 Phosphorylation P00519 (ABL1)
K116 Ubiquitination
T117 Phosphorylation Q9Y243 (AKT3) , P31751 (AKT2) , P31749 (AKT1)
K128 Ubiquitination
K148 Ubiquitination
K161 Ubiquitination
T174 Phosphorylation
K177 Ubiquitination
T180 Phosphorylation Q13188 (STK3) , Q7L7X3 (TAOK1)
T235 Phosphorylation
T240 Phosphorylation
K243 Ubiquitination
K282 Ubiquitination
K298 Ubiquitination
S316 Phosphorylation P53350 (PLK1)
S323 Phosphorylation
T325 Phosphorylation
S333 Phosphorylation
T336 Phosphorylation
S369 Phosphorylation
T378 Phosphorylation Q13188 (STK3)
T384 Phosphorylation P31749 (AKT1)
S385 Phosphorylation P06493 (CDK1)
S392 Phosphorylation
Y396 Phosphorylation
S406 Phosphorylation
C410 S-Nitrosylation
K430 Ubiquitination
S444 Phosphorylation
K453 Ubiquitination
Site PTM Type Enzyme
M1 Acetylation
T3 Phosphorylation
S21 Phosphorylation
K24 Ubiquitination
K35 Acetylation
K35 Ubiquitination
S40 Phosphorylation
Y41 Phosphorylation
S43 Phosphorylation
Y45 Phosphorylation
K46 Ubiquitination
K50 Ubiquitination
K59 Methylation
K59 Ubiquitination
K72 Ubiquitination
S82 Phosphorylation P45983 (MAPK8)
K119 Ubiquitination
T120 Phosphorylation P31749 (AKT1)
S132 Phosphorylation
K151 Ubiquitination
K164 Ubiquitination
T177 Phosphorylation Q13043 (STK4)
K180 Ubiquitination
T183 Phosphorylation Q13043 (STK4) , P26927 (MST1)
T238 Phosphorylation
T243 Phosphorylation
K246 Ubiquitination
K264 Ubiquitination
S265 Phosphorylation
K285 Ubiquitination
S288 Phosphorylation
S307 Phosphorylation
S320 Phosphorylation
S327 Phosphorylation
T329 Phosphorylation
T340 Phosphorylation O14757 (CHEK1)
T353 Phosphorylation
T360 Phosphorylation
T367 Phosphorylation
T380 Phosphorylation
T387 Phosphorylation Q9Y243 (AKT3) , Q13043 (STK4) , P31749 (AKT1) , P31751 (AKT2)
S394 Phosphorylation
Y398 Phosphorylation
K404 Ubiquitination
S410 Phosphorylation
K413 Ubiquitination
S414 Phosphorylation
K420 Ubiquitination
S422 Phosphorylation
S423 Phosphorylation
K426 Ubiquitination
Y433 Phosphorylation P00519 (ABL1)
K437 Ubiquitination
S438 Phosphorylation
T440 Phosphorylation
K446 Ubiquitination

翻译修饰 - Q13043/Q13188 作为激酶

Substrate Site Source
O95835 (LATS1) S872 Uniprot
O95835 (LATS1) S909 Uniprot
O95835 (LATS1) T967 Uniprot
O95835 (LATS1) T1012 Uniprot
O95835 (LATS1) T1060 Uniprot
O95835 (LATS1) T1079 Uniprot
P00519 (ABL1) T735 Uniprot
P17252 (PRKCA) S226 Uniprot
P17252 (PRKCA) T228 Uniprot
P18754 (RCC1) S2 Uniprot
P18754 (RCC1) S11 Uniprot
P46937 (YAP1) S127 Uniprot
P51955 (NEK2) S356 Uniprot
P51955 (NEK2) S365 Uniprot
P51955 (NEK2) S406 Uniprot
P51955 (NEK2) S438 Uniprot
Q13188 (STK3) T180 Uniprot
Q13188 (STK3) T378 Uniprot
Q7L9L4 (MOB1B) T12 Uniprot
Q7L9L4 (MOB1B) T35 Uniprot
Q7L9L4 (MOB1B) T74 Uniprot
Q9H492 (MAP1LC3A) T50 Uniprot
Q9H4B6 (SAV1) T26 Uniprot
Q9H4B6 (SAV1) S27 Uniprot
Q9H4B6 (SAV1) S36 Uniprot
Q9H4B6 (SAV1) S269 Uniprot
Q9H8S9 (MOB1A) T12 Uniprot
Q9H8S9 (MOB1A) T35 Uniprot
Q9H8S9 (MOB1A) T74 Uniprot
Q9NRM7 (LATS2) S872 Uniprot
Q9NRM7 (LATS2) T1041 Uniprot
Substrate Site Source
O43524 (FOXO3) S209 Uniprot
O43524 (FOXO3) S215 Uniprot
O43524 (FOXO3) S231 Uniprot
O43524 (FOXO3) S232 Uniprot
O43524 (FOXO3) S243 Uniprot
O95835 (LATS1) S909 Uniprot
O95835 (LATS1) T1079 Uniprot
P00519 (ABL1) T735 Uniprot
P19429 (TNNI3) T31 Uniprot
P19429 (TNNI3) T51 Uniprot
P19429 (TNNI3) T129 Uniprot
P19429 (TNNI3) T143 Uniprot
P33778 (HIST1H2BB) S15 Uniprot
Q06830 (PRDX1) T18 Uniprot
Q06830 (PRDX1) T90 Uniprot
Q06830 (PRDX1) T183 Uniprot
Q12778 (FOXO1) S212 Uniprot
Q13043-1 (STK4) T177 Uniprot
Q13043 (STK4) T183 Uniprot
Q13043-1 (STK4) T187 Uniprot
Q13043-1 (STK4) S327 Uniprot
Q13043 (STK4) T387 Uniprot
Q14457 (BECN1) T108 Uniprot
Q15208 (STK38) T444 Uniprot
Q7L9L4 (MOB1B) T12 Uniprot
Q7L9L4 (MOB1B) T35 Uniprot
Q96GD4 (AURKB) T232 Uniprot
Q9H492 (MAP1LC3A) T50 Uniprot
Q9H8S9 (MOB1A) T12 Uniprot
Q9H8S9 (MOB1A) T35 Uniprot
Q9NRM7 (LATS2) S872 Uniprot
Q9NRM7 (LATS2) T1041 Uniprot
Q9NS23-2 (RASSF1) T202 Uniprot
Q9NS23-2 (RASSF1) S203 Uniprot

研究背景

功能:

Stress-activated, pro-apoptotic kinase which, following caspase-cleavage, enters the nucleus and induces chromatin condensation followed by internucleosomal DNA fragmentation. Key component of the Hippo signaling pathway which plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. STK3/MST2 and STK4/MST1 are required to repress proliferation of mature hepatocytes, to prevent activation of facultative adult liver stem cells (oval cells), and to inhibit tumor formation (By similarity). Phosphorylates 'Ser-14' of histone H2B (H2BS14ph) during apoptosis. Phosphorylates FOXO3 upon oxidative stress, which results in its nuclear translocation and cell death initiation. Phosphorylates MOBKL1A, MOBKL1B and RASSF2. Phosphorylates TNNI3 (cardiac Tn-I) and alters its binding affinity to TNNC1 (cardiac Tn-C) and TNNT2 (cardiac Tn-T). Phosphorylates FOXO1 on 'Ser-212' and regulates its activation and stimulates transcription of PMAIP1 in a FOXO1-dependent manner. Phosphorylates SIRT1 and inhibits SIRT1-mediated p53/TP53 deacetylation, thereby promoting p53/TP53 dependent transcription and apoptosis upon DNA damage. Acts as an inhibitor of PKB/AKT1. Phosphorylates AR on 'Ser-650' and suppresses its activity by intersecting with PKB/AKT1 signaling and antagonizing formation of AR-chromatin complexes.

翻译修饰:

Autophosphorylated on serine and threonine residues. Phosphorylation at Thr-387 by PKB/AKT1, leads to inhibition of its: kinase activity, nuclear translocation and autophosphorylation at Thr-183. It also diminishes its cleavage by caspases and its ability to phosphorylate FOXO3.

Proteolytically cleaved by caspase-3 during apoptosis at Asp-326 and Asp-349 resulting in a 37 kDa or a 39 kDa subunit respectively. The 39 kDa subunit is further cleaved into the 37 kDa form. Proteolytic cleavage results in kinase activation and nuclear translocation of the truncated form (MST1/N). It is less likely that cleavage at Asp-349 is a prerequisite for activation as this site is not conserved in the murine ortholog.

细胞定位:

Cytoplasm. Nucleus.
Note: The caspase-cleaved form cycles between the nucleus and cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Expressed in prostate cancer and levels increase from the normal to the malignant state (at protein level). Ubiquitously expressed.

亚基结构:

Homodimer; mediated via the coiled-coil region. Interacts with NORE1, which inhibits autoactivation. Interacts with and stabilizes SAV1. Interacts with RASSF1. Interacts with FOXO3. Interacts with RASSF2 (via SARAH domain). Interacts with AR, PKB/AKT1, TNNI3 and SIRT1. Interacts with DLG5 (via PDZ domain 3). Interacts with MARK3 in the presence of DLG5. Interacts with SCRIB in the presence of DLG5.

蛋白家族:

Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.

功能:

Stress-activated, pro-apoptotic kinase which, following caspase-cleavage, enters the nucleus and induces chromatin condensation followed by internucleosomal DNA fragmentation. Key component of the Hippo signaling pathway which plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. STK3/MST2 and STK4/MST1 are required to repress proliferation of mature hepatocytes, to prevent activation of facultative adult liver stem cells (oval cells), and to inhibit tumor formation. Phosphorylates NKX2-1 (By similarity). Phosphorylates NEK2 and plays a role in centrosome disjunction by regulating the localization of NEK2 to centrosome, and its ability to phosphorylate CROCC and CEP250. In conjunction with SAV1, activates the transcriptional activity of ESR1 through the modulation of its phosphorylation. Positively regulates RAF1 activation via suppression of the inhibitory phosphorylation of RAF1 on 'Ser-259'. Phosphorylates MOBKL1A and RASSF2. Phosphorylates MOBKL1B on 'Thr-74'. Acts cooperatively with MOBKL1B to activate STK38.

翻译修饰:

Phosphorylation at Thr-117 and Thr-384 by PKB/AKT1, leads to inhibition of its: cleavage, kinase activity, autophosphorylation at Thr-180, binding to RASSF1 and nuclear translocation, and increase in its binding to RAF1.

Proteolytically cleaved by caspase-3 during apoptosis. Proteolytic cleavage results in kinase activation and nuclear translocation of the truncated form (MST1/N).

细胞定位:

Cytoplasm. Nucleus.
Note: The caspase-cleaved form cycles between nucleus and cytoplasm (By similarity). Phosphorylation at Thr-117 leads to inhibition of nuclear translocation.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Expressed at high levels in adult kidney, skeletal and placenta tissues and at very low levels in adult heart, lung and brain tissues.

亚基结构:

Homodimer; mediated via the coiled-coil region. Interacts with NORE1, which inhibits autoactivation (By similarity). Interacts with and stabilizes SAV1. Interacts with RAF1, which prevents dimerization and phosphorylation. Interacts with RASSF1. Interacts (via SARAH domain) with isoform 1 of NEK2. Interacts with ESR1 only in the presence of SAV1. Interacts with PKB/AKT1. Forms a tripartite complex with MOBKL1B and STK38. Interacts with RASSF2 (via SARAH domain). Interacts with DLG5 (via PDZ domain 3). Interacts with LATS1; this interaction is inhibited in the presence of DLG5. Interacts with MARK3 in the presence of DLG5.

蛋白家族:

Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.

研究领域

· Environmental Information Processing > Signal transduction > MAPK signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Ras signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > FoxO signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Hippo signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Hippo signaling pathway - multiple species.   (View pathway)

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Non-small cell lung cancer.   (View pathway)

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