(2)
(6)
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF9933, RRID:AB_2843127.
展开/折叠
amiloride-sensitive; APNH; APNH1; FLJ42224; Na Li countertransporter; Na(+)/H(+) antiporter; Na(+)/H(+) exchanger 1; Na+ H+ antiporter amiloride-sensitive; Na+ H+ antiporter; Na+ H+ exchanger 1; NHE-1; NHE1; OTTHUMP00000004468; SL9A1_HUMAN; SLC9A1; Sodium hydrogen exchanger 1; Sodium/hydrogen exchanger 1; solute carrier family 9; Solute carrier family 9 member 1; Solute carrier family 9 sodium hydrogen exchanger isoform 1 antiporter Na+ H+ amiloride sensitive; Solute carrier family 9 subfamily A (NHE1 cation proton antiporter 1) member 1; Solute carrier family 9 subfamily A member 1;
抗原和靶标
- P19634 SL9A1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MVLRSGICGLSPHRIFPSLLVVVALVGLLPVLRSHGLQLSPTASTIRSSEPPRERSIGDVTTAPPEVTPESRPVNHSVTDHGMKPRKAFPVLGIDYTHVRTPFEISLWILLACLMKIGFHVIPTISSIVPESCLLIVVGLLVGGLIKGVGETPPFLQSDVFFLFLLPPIILDAGYFLPLRQFTENLGTILIFAVVGTLWNAFFLGGLMYAVCLVGGEQINNIGLLDNLLFGSIISAVDPVAVLAVFEEIHINELLHILVFGESLLNDAVTVVLYHLFEEFANYEHVGIVDIFLGFLSFFVVALGGVLVGVVYGVIAAFTSRFTSHIRVIEPLFVFLYSYMAYLSAELFHLSGIMALIASGVVMRPYVEANISHKSHTTIKYFLKMWSSVSETLIFIFLGVSTVAGSHHWNWTFVISTLLFCLIARVLGVLGLTWFINKFRIVKLTPKDQFIIAYGGLRGAIAFSLGYLLDKKHFPMCDLFLTAIITVIFFTVFVQGMTIRPLVDLLAVKKKQETKRSINEEIHTQFLDHLLTGIEDICGHYGHHHWKDKLNRFNKKYVKKCLIAGERSKEPQLIAFYHKMEMKQAIELVESGGMGKIPSAVSTVSMQNIHPKSLPSERILPALSKDKEEEIRKILRNNLQKTRQRLRSYNRHTLVADPYEEAWNQMLLRRQKARQLEQKINNYLTVPAHKLDSPTMSRARIGSDPLAYEPKEDLPVITIDPASPQSPESVDLVNEELKGKVLGLSRDPAKVAEEDEDDDGGIMMRSKETSSPGTDDVFTPAPSDSPSSQRIQRCLSDPGPHPEPGEGEPFFPKGQ
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P19634 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S5 | Phosphorylation | Uniprot | |
| S11 | Phosphorylation | Uniprot | |
| S18 | Phosphorylation | Uniprot | |
| T42 | Phosphorylation | Uniprot | |
| N75 | N-Glycosylation | Uniprot | |
| Y96 | Phosphorylation | Uniprot | |
| S158 | Phosphorylation | Uniprot | |
| S372 | Phosphorylation | Uniprot | |
| K559 | Ubiquitination | Uniprot | |
| K560 | Ubiquitination | Uniprot | |
| Y577 | Phosphorylation | Uniprot | |
| K583 | Ubiquitination | Uniprot | |
| S599 | Phosphorylation | Uniprot | |
| S602 | Phosphorylation | Uniprot | |
| T603 | Phosphorylation | Uniprot | |
| S605 | Phosphorylation | Uniprot | |
| K612 | Ubiquitination | Uniprot | |
| S616 | Phosphorylation | Uniprot | |
| S624 | Phosphorylation | Uniprot | |
| K625 | Ubiquitination | Uniprot | |
| S648 | Phosphorylation | P31749 (AKT1) | Uniprot |
| T653 | Phosphorylation | O75116 (ROCK2) , Q13464 (ROCK1) | Uniprot |
| Y659 | Phosphorylation | Uniprot | |
| Y683 | Phosphorylation | Uniprot | |
| T685 | Phosphorylation | Uniprot | |
| S693 | Phosphorylation | P28482 (MAPK1) | Uniprot |
| T695 | Phosphorylation | Uniprot | |
| S697 | Phosphorylation | Uniprot | |
| S703 | Phosphorylation | P31749 (AKT1) , Q15418 (RPS6KA1) | Uniprot |
| Y708 | Phosphorylation | Uniprot | |
| T718 | Phosphorylation | Q16539 (MAPK14) | Uniprot |
| S723 | Phosphorylation | Q16539 (MAPK14) | Uniprot |
| S726 | Phosphorylation | Q16539 (MAPK14) | Uniprot |
| S729 | Phosphorylation | Q16539 (MAPK14) | Uniprot |
| K738 | Ubiquitination | Uniprot | |
| K750 | Ubiquitination | Uniprot | |
| S766 | Phosphorylation | Uniprot | |
| S770 | Phosphorylation | P28482 (MAPK1) | Uniprot |
| S771 | Phosphorylation | Uniprot | |
| T774 | Phosphorylation | Uniprot | |
| T779 | Phosphorylation | Uniprot | |
| S783 | Phosphorylation | Uniprot | |
| S785 | Phosphorylation | P28482 (MAPK1) | Uniprot |
| S787 | Phosphorylation | Uniprot | |
| S788 | Phosphorylation | Uniprot | |
| S796 | Phosphorylation | P31749 (AKT1) | Uniprot |
研究背景
Involved in pH regulation to eliminate acids generated by active metabolism or to counter adverse environmental conditions. Major proton extruding system driven by the inward sodium ion chemical gradient. Plays an important role in signal transduction.
O-glycosylated.
Ubiquitinated, leading to its degradation by the proteasome. Ubiquitination is reduced by CHP1 (By similarity).
Membrane>Multi-pass membrane protein. Endoplasmic reticulum membrane>Multi-pass membrane protein. Cell membrane>Multi-pass membrane protein.
Note: Colocalizes with CHP1 at the reticulum endoplasmic (By similarity). Colocalizes with CHP1 and CHP2 at the plasma membrane.
Kidney and intestine.
Oligomer (By similarity). Interacts with CALM1 in a calcium-dependent manner. Interacts with TESC. Interacts (via the juxtamembrane region of the cytoplasmic C-terminal domain) with CHP1; the interaction occurs at the plasma membrane in a calcium-dependent manner. Interacts with CHP2; the interaction occurs in a calcium-dependent manner.
Belongs to the monovalent cation:proton antiporter 1 (CPA1) transporter (TC 2.A.36) family.
研究领域
· Cellular Processes > Cell motility > Regulation of actin cytoskeleton. (View pathway)
· Environmental Information Processing > Signal transduction > cAMP signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > Apelin signaling pathway. (View pathway)
· Human Diseases > Cancers: Overview > Proteoglycans in cancer.
· Organismal Systems > Circulatory system > Cardiac muscle contraction. (View pathway)
· Organismal Systems > Circulatory system > Adrenergic signaling in cardiomyocytes. (View pathway)
· Organismal Systems > Endocrine system > Thyroid hormone signaling pathway. (View pathway)
· Organismal Systems > Digestive system > Salivary secretion.
· Organismal Systems > Digestive system > Gastric acid secretion.
· Organismal Systems > Digestive system > Pancreatic secretion.
文献引用
Application: IHC Species: Rat Sample: myocardial tissues
Application: WB Species: Rat Sample: myocardial tissues
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