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  • 名 称:
    磷酸化 MLKL (Ser358) 抗体
  • 货 号:
    AF7420
  • RRID:
    AB_2843860
  • 来 源:
    Rabbit
  • 应 用:
    WB,IHC,ELISA(peptide)
  • 反 应:
    Human, Mouse, Rat
  • 蛋白号:
  • 分子量:
    54kD;
    54kDa(Calculated).
  • 浓 度:
    1mg/ml
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产品信息

别名:Expand▼

9130019I15Rik; FLJ34389; hMLKL; Mixed lineage kinase domain like; Mixed lineage kinase domain like protein; Mixed lineage kinase domain like pseudokinase; Mixed lineage kinase domain-like protein; Mlkl; MLKL_HUMAN;

应用:

WB 1:500-1:2000, IHC 1:50-1:200, ELISA(peptide) 1:20000-1:40000
*The optimal dilutions should be determined by the end user.

反应性:

Human, Mouse, Rat

来源:

Rabbit

克隆性:

Polyclonal

纯化:

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

特异性:

Phospho-MLKL (Ser358) Antibody detects endogenous levels of MLKL only when phosphorylated at Ser358.

RRID:

AB_2843860
引用格式: Affinity Biosciences Cat# AF7420, RRID:AB_2843860.

形式:

Liquid

浓度:

1mg/ml

保存条件:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.

免疫原

Immunogen:

A synthesized peptide derived from human MLKL around the phosphorylation site of Ser358.

Uniprot:



>>Visit The Human Protein Atlas

Gene ID:

Gene Name:

MLKL

Molecular Weight:

Observed Mol.Wt.: 54kD.
Predicted Mol.Wt.: 54kDa(Calculated)..

Sequence:
MENLKHIITLGQVIHKRCEEMKYCKKQCRRLGHRVLGLIKPLEMLQDQGKRSVPSEKLTTAMNRFKAALEEANGEIEKFSNRSNICRFLTASQDKILFKDVNRKLSDVWKELSLLLQVEQRMPVSPISQGASWAQEDQQDADEDRRAFQMLRRDNEKIEASLRRLEINMKEIKETLRQYLPPKCMQEIPQEQIKEIKKEQLSGSPWILLRENEVSTLYKGEYHRAPVAIKVFKKLQAGSIAIVRQTFNKEIKTMKKFESPNILRIFGICIDETVTPPQFSIVMEYCELGTLRELLDREKDLTLGKRMVLVLGAARGLYRLHHSEAPELHGKIRSSNFLVTQGYQVKLAGFELRKTQTSMSLGTTREKTDRVKSTAYLSPQELEDVFYQYDVKSEIYSFGIVLWEIATGDIPFQGCNSEKIRKLVAVKRQQEPLGEDCPSELREIIDECRAHDPSVRPSVDEILKKLSTFSK

研究背景

Function:

Pseudokinase that plays a key role in TNF-induced necroptosis, a programmed cell death process. Activated following phosphorylation by RIPK3, leading to homotrimerization, localization to the plasma membrane and execution of programmed necrosis characterized by calcium influx and plasma membrane damage. Does not have protein kinase activity. Binds to highly phosphorylated inositol phosphates such as inositolhexakisphosphate (InsP6) which is essential for its necroptotic function.

Post-translational Modifications:

Phosphorylation by RIPK3 induces a conformational switch that is required for necroptosis. It also induces homotrimerization and localization to the plasma membrane.

Subcellular Location:

Cytoplasm. Cell membrane.
Note: Localizes to the cytoplasm and translocates to the plasma membrane on necroptosis induction.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location

Subunit Structure:

Homooligomer. Homotrimer; forms homotrimers on necroptosis induction. Interacts with RIPK3; the interaction is direct. Upon TNF-induced necrosis, forms in complex with PGAM5, RIPK1 and RIPK3. Within this complex, may play a role in the proper targeting of RIPK1/RIPK3 to its downstream effector PGAM5.

Similarity:

The protein kinase domain is catalytically inactive but contains an unusual pseudoactive site with an interaction between Lys-230 and Gln-356 residues. Upon phosphorylation by RIPK3, undergoes an active conformation (By similarity).

The coiled coil region 2 is responsible for homotrimerization.

Belongs to the protein kinase superfamily.

研究领域

Research Fields:

· Cellular Processes > Cell growth and death > Necroptosis.(View pathway)
· Environmental Information Processing > Signal transduction > TNF signaling pathway.(View pathway)

Reference Citations:

1). Hu B;Zhang S;Liu W;Wang P;Chen S;Lv X;Shi D;Ma K;Wang B;Wu Y;Shao Z; et al. Inhibiting Heat Shock Protein 90 Protects Nucleus Pulposus-Derived Stem/Progenitor Cells From Compression-Induced Necroptosis and Apoptosis. Front Cell Dev Biol 2020 Aug 7;8:685. (PubMed: 32850811) [IF=5.201]

2). Huang Y;Feng Y;Cui L;Yang L;Zhang Q;Zhang J;Jiang X;Zhang X;Lv Y;Jia JZ;Zhang DX;Huang YS; et al. Autophagy-related LC3 accumulation interacted directly with LIR containing RIPK1 and RIPK3, stimulating necroptosis in hypoxic cardiomyocytes. Front Cell Dev Biol 2021 Jul 23;9:679637. (PubMed: 34368130) [IF=5.201]

3). Wang R;Wei L;Dong Z;Meng F;Wang G;Zhou S;Lan X;Liao Z;Chen M; et al. Pterocephin A, a Novel Triterpenoid Saponin from Pterocephalus hookeri Induced Liver Injury by Activation of Necroptosis. Phytomedicine 2021 May;85:153548. (PubMed: 33831690) [IF=4.268]

4). Zhang L;Gao H;Yang L;Liu T;Zhang Q;Xun J;Li C;Cui L;Wang X; et al. FTY720 induces neutrophil extracellular traps via a NADPH oxidase-independent pathway. Arch Biochem Biophys 2021 Aug 23;711:109015. (PubMed: 34437865) [IF=3.391]

5). Xu Y;Tu W;Sun D;Chen X;Ge Y;Yao S;Li B;Zhenbo Zhang None;Liu Y; et al. Nrf2 alleviates radiation-induced rectal injury by inhibiting of necroptosis. Biochem Biophys Res Commun 2021 Mar 25;554:49-55. (PubMed: 33774279)

6). Wang W;Xie L;Zou X;Hu W;Tian X;Zhao G;Chen M; et al. Pomelo peel oil suppresses TNF-α-induced necroptosis and cerebral ischaemia–reperfusion injury in a rat model of cardiac arrest. Pharm Biol 2021 Dec;59(1):401-409. (PubMed: 33794116)

7). Marunouchi T;Ito T;Onda S;Kyo L;Takahashi K;Uchida M;Yano E;Tanonaka K; et al. Effects of 17-AAG on the RIP1/RIP3/MLKL pathway during the development of heart failure following myocardial infarction in rats. J Pharmacol Sci 2021 Oct;147(2):192-199. (PubMed: 34384567)

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Catalog Number :

AF7420-BP
(Blocking peptide available as AF7420-BP)

Price/Size :

¥2000/1mg.
Tips: For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).

Function :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (immunoblot) and immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or immunostaining performed with the neutralized antibody.

Format and storage :

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.

Precautions :

This product is for research use only. Not for use in diagnostic or therapeutic procedures.

Pig
0%
Horse
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Bovine
0%
Sheep
0%
Dog
0%
Xenopus
0%
Zebrafish
0%
Chicken
0%
Rabbit
0%
High similarity Medium similarity Low similarity No similarity
Q8NB16 as Substrate
Site PTM Type Enzyme
K40 Ubiquitination
K50 Ubiquitination
S52 Phosphorylation
K57 Ubiquitination
T59 Phosphorylation
K66 Ubiquitination
K78 Ubiquitination
S92 Phosphorylation
S106 Phosphorylation
S125 Phosphorylation
S128 Phosphorylation
K157 Ubiquitination
S161 Phosphorylation
K173 Ubiquitination
K183 Ubiquitination
K198 Ubiquitination
K219 Ubiquitination
K230 Ubiquitination
T246 Phosphorylation
K249 Ubiquitination
T302 Phosphorylation
K331 Ubiquitination
S334 Phosphorylation
K354 Acetylation
K354 Ubiquitination
T357 Phosphorylation Q9Y572 (RIPK3)
S358 Phosphorylation Q9Y572 (RIPK3)
T364 Phosphorylation
K372 Ubiquitination
S373 Phosphorylation
T374 Phosphorylation
S393 Phosphorylation
S417 Phosphorylation
S467 Phosphorylation
IMPORTANT: For western blots, incubate membrane with diluted antibody in 5% w/v milk, 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking, overnight.

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