HES1 Antibody - #AF7575

(2)   (4)  
产品: HES1 抗体
货号: AF7575
描述: Rabbit polyclonal antibody to HES1
应用: WB
反应: Human, Mouse, Rat
预测: Pig, Bovine, Horse, Sheep, Rabbit, Chicken, Xenopus
分子量: 30kDa; 30kD(Calculated).
蛋白号: Q14469
RRID: AB_2843939

浏览相似产品>>

   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

联系销售
相关下载
MS Report
HPLC Report
MSDS
说明书
COA
实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Chicken(100%), Xenopus(100%)
克隆:
Polyclonal
特异性:
HES1 Antibody detects endogenous levels of total HES1.
RRID:
AB_2843939
引用格式: Affinity Biosciences Cat# AF7575, RRID:AB_2843939.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

bHLHb39; C-HAIRY1; c-hairy1A; Class B basic helix-loop-helix protein 39; FLJ20408; Hairy and enhancer of split 1 (Drosophila); Hairy and enhancer of split 1; Hairy homolog (Drosophila); Hairy homolog; Hairy like; Hairy, Drosophila, homolog of; Hairy-like protein; Hairy/enhancer of split, Drosophila, homolog of, 1; HAIRY1; HES-1; hes1; Hes1 hairy and enhancer of split 1 (Drosophila); HES1_HUMAN; HHL; HL; HRY; MGC129109; OTTHUMP00000209031; RHL; Transcription factor HES-1;

抗原和靶标

免疫原:
Uniprot:

Q14469(HES1_HUMAN) >>Visit HPA database.

基因/基因ID:
HES1(3280)
序列:
MPADIMEKNSSSPVAATPASVNTTPDKPKTASEHRKSSKPIMEKRRRARINESLSQLKTLILDALKKDSSRHSKLEKADILEMTVKHLRNLQRAQMTAALSTDPSVLGKYRAGFSECMNEVTRFLSTCEGVNTEVRTRLLGHLANCMTQINAMTYPGQPHPALQAPPPPPPGPGGPQHAPFAPPPPLVPIPGGAAPPPGGAPCKLGSQAGEAAKVFGGFQVVPAPDGQFAFLIPNGAFAHSGPVIPVYTSNSGTSVGPNAVSPSSGPSLTADSMWRPWRN

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Xenopus
100
Chicken
100
Rabbit
100
Dog
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - Q14469 作为底物

Site PTM Type Enzyme
S10 Phosphorylation
S11 Phosphorylation
S12 Phosphorylation
T24 Phosphorylation
S32 Phosphorylation
S37 Phosphorylation P17252 (PRKCA)
S38 Phosphorylation P17252 (PRKCA)
S55 Phosphorylation
T59 Phosphorylation
K77 Ubiquitination
K86 Ubiquitination
T97 Phosphorylation
K109 Ubiquitination

研究背景

功能:

Transcriptional repressor of genes that require a bHLH protein for their transcription. May act as a negative regulator of myogenesis by inhibiting the functions of MYOD1 and ASH1. Binds DNA on N-box motifs: 5'-CACNAG-3' with high affinity and on E-box motifs: 5'-CANNTG-3' with low affinity (By similarity). May play a role in a functional FA core complex response to DNA cross-link damage, being required for the stability and nuclear localization of FA core complex proteins, as well as for FANCD2 monoubiquitination in response to DNA damage.

细胞定位:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
亚基结构:

Transcription repression requires formation of a complex with a corepressor protein of the Groucho/TLE family. Interacts (via WPRW motif) with TLE1, and more weakly with TLE2. Interacts with HES6 (By similarity). Interacts with SIRT1. Interacts with an FA complex, composed of FANCA, FANCF, FANCG and FANCL, but not of FANCC, nor FANCE.

蛋白家族:

Has a particular type of basic domain (presence of a helix-interrupting proline) that binds to the N-box (CACNAG), rather than the canonical E-box (CANNTG).

The C-terminal WRPW motif is a transcriptional repression domain necessary for the interaction with Groucho/TLE family members, transcriptional corepressors recruited to specific target DNA by Hairy-related proteins.

The bHLH, as well as cooperation between the central Orange domain and the C-terminal WRPW motif, is required for transcriptional repressor activity.

研究领域

· Environmental Information Processing > Signal transduction > Notch signaling pathway.   (View pathway)

· Genetic Information Processing > Replication and repair > Fanconi anemia pathway.

· Human Diseases > Endocrine and metabolic diseases > Maturity onset diabetes of the young.

· Human Diseases > Infectious diseases: Viral > Human papillomavirus infection.

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Breast cancer.   (View pathway)

文献引用

1). γ‐secretase inhibitors suppress IL‐20‐mediated osteoclastogenesis via Notch signaling and are affected by Notch2 in vitro. SCANDINAVIAN JOURNAL OF IMMUNOLOGY (PubMed: 35384009) [IF=3.7]

Application: WB    Species: Human    Sample:

FIGURE 2 20 ng/mL IL-20 treated the pre-osteoclasts for 48 h meanwhile control groups were treated with 0 ng/mL IL-20. Detected mRNA and analysed by GO Analysis and Pathway Analysis. (A) Bubble chart analysis based on KEGG databases. (B) Gene Set Enrichment Analysis of osteoclast differentiation with control groups and IL-20 treat groups. (C) Gene heatmap showed four classical gene were enriched to Notch signal pathway (Red – up regulated and green – down regulated). (D-E) The expression of Notch1 and Notch2 mRNA with different concentration of IL-20 stimulation. Analysed by one-way ANOVA with Tukey's post hoc analysis with three independent experiments. *P < .05 vs the control group; ns, not significant. (F) mRNA expression of Notch1, Notch2, Hes1 and Hey1 at 20 ng/mL IL-20 stimulation using by qRT-PCR and analysed by Student's t test. Bars represent mean ± SEM from three independent experiments. *P < .05 vs the control group; ns, not significant. (G-H) Western blotting data and analysis of Notch signal pathway. Student's t test was used to detect difference. Bars represent mean ± SEM from three independent experiments. *P < .05 vs the control group; ns, not significant
2). Notch1/Hes1‑PTEN/AKT/IL‑17A feedback loop regulates Th17 cell differentiation in mouse psoriasis‑like skin inflammation. Molecular Medicine Reports (PubMed: 35582997) [IF=3.4]

Application: WB    Species: mouse    Sample:

Figure 9. - Protein levels of (A) hairy and enhancer of split 1, (B) PTEN, (C-1) p-AKT (Thr308), (C-2) AKT, (C-3) p-AKT (Ser473), (D-1) mTORC1, (D-2) p-mTOR (Ser2448), (E) IL-17A in mouse splenic mononuclear cells of control group, 0 µM LY294002 group, 10 µM LY294002 group and 50 µM LY294002 group. **P

限制条款

产品的规格、报价、验证数据请以官网为准，官网链接：www.affbiotech.com | www.affbiotech.cn（简体中文）| www.affbiotech.jp（日本語）

产品的数据信息为Affinity所有，未经授权不得收集Affinity官网数据或资料用于商业用途，对抄袭产品数据的行为我们将保留诉诸法律的权利。

产品相关数据会因产品批次、产品检测情况随时调整，如您已订购该产品，请以订购时随货说明书为准，否则请以官网内容为准，官网内容有改动时恕不另行通知。

Affinity保证所销售产品均经过严格质量检测。如您购买的商品在规定时间内出现问题需要售后时，请您在Affinity官方渠道提交售后申请。

产品仅供科学研究使用。不用于诊断和治疗。 

产品未经授权不得转售。

Affinity Biosciences将不会对在使用我们的产品时可能发生的专利侵权或其他侵权行为负责。Affinity Biosciences, Affinity Biosciences标志和所有其他商标所有权归Affinity Biosciences LTD.