产品: LATS1 抗体
货号: AF7670
描述: Rabbit polyclonal antibody to LATS1
应用: WB
反应: Human, Mouse
预测: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Chicken, Xenopus
分子量: 160kDa; 127kD(Calculated).
蛋白号: O95835
RRID: AB_2844034

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse
预测:
Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Chicken(100%), Xenopus(100%)
克隆:
Polyclonal
特异性:
LATS1 Antibody detects endogenous levels of total LATS1.
RRID:
AB_2844034
引用格式: Affinity Biosciences Cat# AF7670, RRID:AB_2844034.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

h-warts; Large tumor suppressor homolog 1; LATS large tumor suppressor homolog 1; LATS1; LATS1_HUMAN; Serine threonine protein kinase LATS1; Serine/threonine-protein kinase LATS1; WARTS; WARTS protein kinase; wts;

抗原和靶标

免疫原:
Uniprot:
基因/基因ID:
表达:
O95835 LATS1_HUMAN:

Expressed in all adult tissues examined except for lung and kidney.

序列:
MKRSEKPEGYRQMRPKTFPASNYTVSSRQMLQEIRESLRNLSKPSDAAKAEHNMSKMSTEDPRQVRNPPKFGTHHKALQEIRNSLLPFANETNSSRSTSEVNPQMLQDLQAAGFDEDMVIQALQKTNNRSIEAAIEFISKMSYQDPRREQMAAAAARPINASMKPGNVQQSVNRKQSWKGSKESLVPQRHGPPLGESVAYHSESPNSQTDVGRPLSGSGISAFVQAHPSNGQRVNPPPPPQVRSVTPPPPPRGQTPPPRGTTPPPPSWEPNSQTKRYSGNMEYVISRISPVPPGAWQEGYPPPPLNTSPMNPPNQGQRGISSVPVGRQPIIMQSSSKFNFPSGRPGMQNGTGQTDFMIHQNVVPAGTVNRQPPPPYPLTAANGQSPSALQTGGSAAPSSYTNGSIPQSMMVPNRNSHNMELYNISVPGLQTNWPQSSSAPAQSSPSSGHEIPTWQPNIPVRSNSFNNPLGNRASHSANSQPSATTVTAITPAPIQQPVKSMRVLKPELQTALAPTHPSWIPQPIQTVQPSPFPEGTASNVTVMPPVAEAPNYQGPPPPYPKHLLHQNPSVPPYESISKPSKEDQPSLPKEDESEKSYENVDSGDKEKKQITTSPITVRKNKKDEERRESRIQSYSPQAFKFFMEQHVENVLKSHQQRLHRKKQLENEMMRVGLSQDAQDQMRKMLCQKESNYIRLKRAKMDKSMFVKIKTLGIGAFGEVCLARKVDTKALYATKTLRKKDVLLRNQVAHVKAERDILAEADNEWVVRLYYSFQDKDNLYFVMDYIPGGDMMSLLIRMGIFPESLARFYIAELTCAVESVHKMGFIHRDIKPDNILIDRDGHIKLTDFGLCTGFRWTHDSKYYQSGDHPRQDSMDFSNEWGDPSSCRCGDRLKPLERRAARQHQRCLAHSLVGTPNYIAPEVLLRTGYTQLCDWWSVGVILFEMLVGQPPFLAQTPLETQMKVINWQTSLHIPPQAKLSPEASDLIIKLCRGPEDRLGKNGADEIKAHPFFKTIDFSSDLRQQSASYIPKITHPTDTSNFDPVDPDKLWSDDNEEENVNDTLNGWYKNGKHPEHAFYEFTFRRFFDDNGYPYNYPKPIEYEYINSQGSEQQSDEDDQNTGSEIKNRDLVYV

种属预测

种属预测:

score>80的预测可信度较高,可尝试用于WB检测。*预测模型主要基于免疫原序列比对,结果仅作参考,不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Xenopus
100
Zebrafish
100
Chicken
100
Rabbit
100
Dog
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - O95835 作为底物

Site PTM Type Enzyme
T17 Phosphorylation
Y23 Phosphorylation
S26 Phosphorylation
S27 Phosphorylation
S37 Phosphorylation
S42 Phosphorylation
K76 Ubiquitination
S84 Phosphorylation
Y143 Phosphorylation
S171 Phosphorylation
S177 Phosphorylation
S181 Phosphorylation
R243 Methylation
S244 Phosphorylation
T246 Phosphorylation
R252 Methylation
T255 Phosphorylation
R259 Methylation
T261 Phosphorylation
T262 Phosphorylation
K275 Methylation
Y277 Phosphorylation
S278 Phosphorylation
Y283 Phosphorylation
S336 Phosphorylation
S462 Phosphorylation
S464 Phosphorylation O60285 (NUAK1) , Q9H093 (NUAK2)
T490 Phosphorylation P06493 (CDK1)
S580 Phosphorylation
S596 Phosphorylation
T612 Phosphorylation
S613 Phosphorylation P06493 (CDK1)
T616 Phosphorylation
S633 Phosphorylation
S635 Phosphorylation
K662 Ubiquitination
S674 Phosphorylation O95835 (LATS1)
K688 Ubiquitination
K728 Ubiquitination
K734 Ubiquitination
K751 Sumoylation
S792 Phosphorylation
K830 Sumoylation
S864 Phosphorylation
S872 Phosphorylation Q13188 (STK3)
S909 Phosphorylation Q13043 (STK4) , Q13188 (STK3) , O95835 (LATS1)
T967 Phosphorylation Q13188 (STK3)
K976 Ubiquitination
S982 Phosphorylation
K1005 Ubiquitination
T1012 Phosphorylation Q13188 (STK3)
K1029 Ubiquitination
K1046 Ubiquitination
S1049 Phosphorylation O95835 (LATS1)
T1060 Phosphorylation Q13188 (STK3)
Y1076 Phosphorylation
T1079 Phosphorylation Q13043 (STK4) , Q13188 (STK3)
S1107 Phosphorylation
S1111 Phosphorylation

翻译修饰 - O95835 作为激酶

Substrate Site Source
O14974 (PPP1R12A) S445 Uniprot
O95835 (LATS1) S674 Uniprot
O95835 (LATS1) S909 Uniprot
O95835 (LATS1) S1049 Uniprot
P04049 (RAF1) S259 Uniprot
P46937-2 (YAP1) S61 Uniprot
P46937-2 (YAP1) S109 Uniprot
P46937 (YAP1) S127 Uniprot
P46937-2 (YAP1) S164 Uniprot
P46937-2 (YAP1) S381 Uniprot
P46937 (YAP1) S397 Uniprot
Q02241 (KIF23) S716 Uniprot
Q02241 (KIF23) S814 Uniprot
Q8NHZ8 (CDC26) T7 Uniprot
Q9GZV5 (WWTR1) S89 Uniprot
Q9NQS7 (INCENP) S894 Uniprot

研究背景

功能:

Negative regulator of YAP1 in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS1 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. Acts as a tumor suppressor which plays a critical role in maintenance of ploidy through its actions in both mitotic progression and the G1 tetraploidy checkpoint. Negatively regulates G2/M transition by down-regulating CDK1 kinase activity. Involved in the control of p53 expression. Affects cytokinesis by regulating actin polymerization through negative modulation of LIMK1. May also play a role in endocrine function. Plays a role in mammary gland epithelial cells differentiation, both through the Hippo signaling pathway and the intracellular estrogen receptor signaling pathway by promoting the degradation of ESR1.

翻译修饰:

Autophosphorylated and phosphorylated during M-phase of the cell cycle. Phosphorylated by STK3/MST2 at Ser-909 and Thr-1079, which results in its activation. Phosphorylation at Ser-464 by NUAK1 and NUAK2 leads to decreased protein level and is required to regulate cellular senescence and cellular ploidy.

细胞定位:

Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome.
Note: Localizes to the centrosomes throughout interphase but migrates to the mitotic apparatus, including spindle pole bodies, mitotic spindle, and midbody, during mitosis.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Expressed in all adult tissues examined except for lung and kidney.

亚基结构:

Complexes with CDK1 in early mitosis. LATS1-associated CDK1 has no mitotic cyclin partner and no apparent kinase activity. Binds phosphorylated ZYX, locating this protein to the mitotic spindle and suggesting a role for actin regulatory proteins during mitosis. Binds to and colocalizes with LIMK1 at the actomyosin contractile ring during cytokinesis. Interacts (via PPxY motif 2) with YAP1 (via WW domains). Interacts with MOB1A and MOB1B. Interacts with LIMD1, WTIP and AJUBA. Interacts with ESR1, DCAF1 and DCAF13; probably recruits DCAF1 and DCAF13 to ESR1 to promote ESR1 ubiquitination and ubiquitin-mediated proteasomal degradation. Interacts with STK3/MST2; this interaction is inhibited in the presence of DLG5. Interacts with SCRIB in the presence of DLG5.

蛋白家族:

Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.

研究领域

· Environmental Information Processing > Signal transduction > Hippo signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Hippo signaling pathway - multiple species.   (View pathway)

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