产品: ACSL4/FACL4 抗体
货号: DF12141
描述: Rabbit polyclonal antibody to ACSL4/FACL4
应用: WB IHC IF/ICC
反应: Human, Mouse, Rat
预测: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
分子量: 79 kDa,74 kDa; 79kD(Calculated).
蛋白号: O60488
RRID: AB_2844946

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(83%)
克隆:
Polyclonal
特异性:
ACSL4/FACL4 Antibody detects endogenous levels of total ACSL4/FACL4.
RRID:
AB_2844946
引用格式: Affinity Biosciences Cat# DF12141, RRID:AB_2844946.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

ACS 4; ACS4; ACSL 4; Acsl4; ACSL4_HUMAN; acyl CoA synthetase 4; Acyl CoA synthetase long chain family member 4; FACL 4; FACL4; Fatty acid Coenzyme A ligase; fatty acid Coenzyme A ligase long-chain 4; LACS 4; LACS4; Lignoceroyl CoA synthase; Long chain 4; long chain acyl CoA synthetase 4; long chain fatty acid CoA ligase 4; long chain fatty acid Coenzyme A ligase 4; Long-chain acyl-CoA synthetase 4; Long-chain-fatty-acid--CoA ligase 4; MRX63; MRX68;

抗原和靶标

免疫原:
Uniprot:
基因/基因ID:
序列:
MKLKLNVLTIILLPVHLLITIYSALIFIPWYFLTNAKKKNAMAKRIKAKPTSDKPGSPYRSVTHFDSLAVIDIPGADTLDKLFDHAVSKFGKKDSLGTREILSEENEMQPNGKVFKKLILGNYKWMNYLEVNRRVNNFGSGLTALGLKPKNTIAIFCETRAEWMIAAQTCFKYNFPLVTLYATLGKEAVVHGLNESEASYLITSVELLESKLKTALLDISCVKHIIYVDNKAINKAEYPEGFEIHSMQSVEELGSNPENLGIPPSRPTPSDMAIVMYTSGSTGRPKGVMMHHSNLIAGMTGQCERIPGLGPKDTYIGYLPLAHVLELTAEISCFTYGCRIGYSSPLTLSDQSSKIKKGSKGDCTVLKPTLMAAVPEIMDRIYKNVMSKVQEMNYIQKTLFKIGYDYKLEQIKKGYDAPLCNLLLFKKVKALLGGNVRMMLSGGAPLSPQTHRFMNVCFCCPIGQGYGLTESCGAGTVTEVTDYTTGRVGAPLICCEIKLKDWQEGGYTINDKPNPRGEIVIGGQNISMGYFKNEEKTAEDYSVDENGQRWFCTGDIGEFHPDGCLQIIDRKKDLVKLQAGEYVSLGKVEAALKNCPLIDNICAFAKSDQSYVISFVVPNQKRLTLLAQQKGVEGTWVDICNNPAMEAEILKEIREAANAMKLERFEIPIKVRLSPEPWTPETGLVTDAFKLKRKELRNHYLKDIERMYGGK

种属预测

种属预测:

score>80的预测可信度较高,可尝试用于WB检测。*预测模型主要基于免疫原序列比对,结果仅作参考,不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Chicken
100
Rabbit
100
Xenopus
83
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - O60488 作为底物

Site PTM Type Enzyme
S23 Phosphorylation
Y31 Phosphorylation
T34 Phosphorylation
K47 Ubiquitination
K49 Ubiquitination
K54 Ubiquitination
S57 Phosphorylation
T63 Phosphorylation
K89 Acetylation
K89 Ubiquitination
K92 Acetylation
S95 Phosphorylation
K113 Ubiquitination
K117 Ubiquitination
S140 Phosphorylation
T143 Phosphorylation
K148 Ubiquitination
K150 Ubiquitination
K211 Ubiquitination
C221 S-Nitrosylation
K223 Ubiquitination
Y227 Phosphorylation
K231 Ubiquitination
K312 Ubiquitination
S352 Phosphorylation
S353 Phosphorylation
K354 Ubiquitination
K356 Ubiquitination
K360 Ubiquitination
K367 Ubiquitination
K383 Ubiquitination
K388 Ubiquitination
K397 Acetylation
K397 Ubiquitination
K401 Acetylation
K401 Ubiquitination
Y404 Phosphorylation
K407 Ubiquitination
K413 Ubiquitination
Y415 Phosphorylation
K426 Ubiquitination
S447 Phosphorylation
Y483 Phosphorylation
T485 Phosphorylation
K498 Ubiquitination
K500 Ubiquitination
T508 Phosphorylation
K512 Ubiquitination
K536 Ubiquitination
Y541 Phosphorylation
Y582 Phosphorylation
S584 Phosphorylation
K587 Ubiquitination
K593 Ubiquitination
S607 Phosphorylation
K621 Ubiquitination
K651 Ubiquitination
K661 Ubiquitination
K670 Ubiquitination
S674 Phosphorylation
T679 Phosphorylation
T682 Phosphorylation
T686 Phosphorylation
K690 Acetylation
K690 Ubiquitination
K702 Ubiquitination

研究背景

功能:

Catalyzes the conversion of long-chain fatty acids to their active form acyl-CoA for both synthesis of cellular lipids, and degradation via beta-oxidation. Preferentially activates arachidonate and eicosapentaenoate as substrates. Preferentially activates 8,9-EET > 14,15-EET > 5,6-EET > 11,12-EET. Modulates glucose-stimulated insulin secretion by regulating the levels of unesterified EETs (By similarity). Modulates prostaglandin E2 secretion.

细胞定位:

Mitochondrion outer membrane>Single-pass type III membrane protein. Peroxisome membrane>Single-pass type III membrane protein. Microsome membrane>Single-pass type III membrane protein. Endoplasmic reticulum membrane>Single-pass type III membrane protein. Cell membrane.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
蛋白家族:

Belongs to the ATP-dependent AMP-binding enzyme family.

研究领域

· Cellular Processes > Transport and catabolism > Peroxisome.   (View pathway)

· Cellular Processes > Cell growth and death > Ferroptosis.   (View pathway)

· Metabolism > Lipid metabolism > Fatty acid biosynthesis.

· Metabolism > Lipid metabolism > Fatty acid degradation.

· Metabolism > Global and overview maps > Metabolic pathways.

· Metabolism > Global and overview maps > Fatty acid metabolism.

· Organismal Systems > Endocrine system > PPAR signaling pathway.

· Organismal Systems > Endocrine system > Adipocytokine signaling pathway.

文献引用

1). d-Borneol enhances cisplatin sensitivity via autophagy dependent EMT signaling and NCOA4-mediated ferritinophagy. PHYTOMEDICINE (PubMed: 36030746) [IF=7.9]

2). Human umbilical cord mesenchymal stem cells ameliorate erectile dysfunction in rats with diabetes mellitus through the attenuation of ferroptosis. Stem Cell Research & Therapy (PubMed: 36064453) [IF=7.5]

3). Astragaloside IV attenuates myocardial dysfunction in diabetic cardiomyopathy rats through downregulation of CD36-mediated ferroptosis. Phytotherapy Research (PubMed: 36882189) [IF=7.2]

4). Erythropoietin inhibits ferroptosis and ameliorates neurological function after spinal cord injury. Neural Regeneration Research (PubMed: 36204858) [IF=6.1]

Application: WB    Species: Rat    Sample: spinal cord

Figure 3 EPO regulates the expression of ferroptotic biomarkers after SCI. (A) Western blot of the indicated proteins in injured tissues from the treatment groups. (B–H) Quantitative analysis of Tfr, Fpn, Fth, Acsl4 and 4-Hne protein expression at 14 dpi. Gapdh was used as the reference protein. (I, J) Quantitative reverse transcription polymerase chain reaction results of xCT and Gpx4 mRNA extracted from injured tissue at 7 dpi. β-Actin mRNA was used as the reference gene. (K) Quantitative analysis of reduced GSH from injured tissue at 7 dpi. All data are expressed as the mean ± SD (n = 5 in each group). *P < 0.05, **P < 0.01, ***P < 0.001 (one-way analysis of variance followed by Dunnett’s multiple comparisons test). 4-Hne: 4-Hydroxynonenal; Acsl4: acyl-coenzyme A synthetase long chain family member 4; dpi: day(s) post injury; EPO: erythropoietin; Fpn: ferroportin or solute carrier family 40 member 1; Fth: ferritin heavy chain; Gapdh: glyceraldehyde-3-phosphate dehydrogenase; Gpx4: glutathione peroxidase 4; GSH: glutathione; ns: not significant; SCI: spinal cord injury; Tfr: transferrin receptor; xCT: the solute carrier family 7 member 11.

5). Herceptin induces ferroptosis and mitochondrial dysfunction in H9c2 cells. International Journal of Molecular Medicine (PubMed: 34935058) [IF=5.4]

Application: WB    Species: Rat    Sample: H9c2 cells

Figure 3 Fer-1 protects H9c2 cells against Herceptin-induced cell injury and ferroptosis. Fer-1 and DFO reversed the (A) Herceptin-induced reduction in cell viability, (B) Herceptin-induced decrease in GPX4 and SLC7A11 protein expression and Herceptin-induced increase in ACSL4 protein expression. Fer-1 and DFO reversed the Herceptin-induced (C) reduction in GSH content. (D) Fer-1 and DFO did not affect GSSG content. (E) Fer-1 and DFO reversed the Herceptin-induced reduction in the ratio of GSH/GSSG in H9c2 cells. Fer-1 and DFO reversed the Herceptin-induced increase in (F) intracellular and (G) mitochondrial iron levels in H9c2 cells. However, compared with DFO, the effects of Fer-1 were less potent. **P<0.01 and ***P<0.001 vs. NC. #P<0.05 and ##P<0.01 vs. Herceptin (10 µM). Fer-1, ferrostatin-1; GPX4, glutathione peroxidase 4; SLC7A11, recombinant solute carrier family 7 member 11; ACSL4, acyl-CoA synthetase long chain family member 4; GSH, reduced glutathione; GSSG, oxidized glutathione; DFO, deferoxamine; OD, optical density.

Application: WB    Species: rat    Sample: H9c2 cells

Figure 3. | Fer‑1 protects H9c2 cells against Herceptin‑induced cell injury and ferroptosis. Fer‑1 and DFO reversed the (A) Herceptin‑induced reduction in cell viability, (B) Herceptin‑induced decrease in GPX4 and SLC7A11 protein expression and Herceptin‑induced increase in ACSL4 protein expression.

6). Proteomic analysis reveals that ACSL4 activation during reflux esophagitis contributes to ferroptosis-mediated esophageal mucosal damage. European Journal of Pharmacology (PubMed: 35921957) [IF=5.0]

7). 1, 3‐Dichloro‐2‐propanol induced ferroptosis through Nrf2/ARE signaling pathway in hepatocytes. ENVIRONMENTAL TOXICOLOGY (PubMed: 35870111) [IF=4.5]

8). Ferroptosis is involved in corpus cavernosum smooth muscle cells impairment in diabetes mellitus-induced erectile dysfunction. Andrology (PubMed: 36098277) [IF=4.5]

Application: IHC    Species: Rat    Sample: CCSMCs

FIGURE 5 The expression level of GPX4, ACSL4, and SLC7A11: (A) representative results of immunohistochemistry (IHC) and relative expression of GPX4; (B) representative results of IHC and relative expression of ACSL4; and (C) representative results of IHC and relative expression of SLC7A11. *p < 0.05 compared with the control group

9). Liraglutide Alleviates Cognitive Deficit in db/db Mice: Involvement in Oxidative Stress, Iron Overload, and Ferroptosis. NEUROCHEMICAL RESEARCH (PubMed: 34480710) [IF=4.4]

10). Targeting Ferroptosis Attenuates Inflammation, Fibrosis, and Mast Cell Activation in Chronic Prostatitis. Journal of Immunology Research (PubMed: 35755168) [IF=4.1]

Application: WB    Species: Rat    Sample:

Figure 4 DFO and EDA attenuated ferroptosis in EAP model. (a) The iron concentration of prostate lysates was determined by the commercial kit. Results were normalized to protein concentration. (b) The mRNA levels of ferroptosis biomarkers GPX4, SLC7A11, ACSL4, PTGS2, and DHODH relative to internal control were determined by the RT-PCR method. (c) The protein levels of ferroptosis biomarkers GPX4, SLC7A11, ACSL4, LPCAT3, and DHODH were determined by the western blot method. The relative quantification result of each band was performed relative to β-actin. Data was presented as mean ± SEM. #P < 0.05 versus the control group; ##P < 0.01 versus the control group; ###P < 0.001 versus the control group; ∗P < 0.05 versus the EAP group; ∗∗P < 0.01 versus the EAP group; ∗∗∗P < 0.001 versus the EAP group.

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