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¥1200 50ul
¥2200 100ul

当天发货

  • 名 称:
    EYFP-Tag 抗体
  • 货 号:
    BF0725
  • RRID:
    AB_2846200
  • 来 源:
    Mouse
  • 应 用:
    WB
  • 反 应:
    All
  • 浓 度:
    1mg/ml

产品信息

应用:

WB 1:1000-1:10000
*The optimal dilutions should be determined by the end user.

反应性:

All

来源:

Mouse

克隆性:

Monoclonal

克隆号:

AFB17787

纯化:

Affinity-chromatography.

RRID:

AB_2846200
引用格式: Affinity Biosciences Cat# BF0725, RRID:AB_2846200.

形式:

Liquid

浓度:

1mg/ml

保存条件:

Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.

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Catalog Number :

BF0725-BP
(Blocking peptide available as BF0725-BP)

Price/Size :

¥2000/1mg.
Tips: For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).

Function :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (immunoblot) and immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or immunostaining performed with the neutralized antibody.

Format and storage :

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.

Precautions :

This product is for research use only. Not for use in diagnostic or therapeutic procedures.

IMPORTANT: For western blots, incubate membrane with diluted antibody in 5% w/v milk, 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking, overnight.

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