产品: 磷酸化 CaMKII alpha/delta (Thr286) 抗体
货号: AF3493
描述: Rabbit polyclonal antibody to Phospho-CaMKII alpha/delta (Thr286)
应用: WB IHC IF/ICC
反应: Human, Mouse, Rat
预测: Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
分子量: 50kDa; 54kD,56kD(Calculated).
蛋白号: Q9UQM7 | Q13557
RRID: AB_2834931

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Zebrafish(88%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(88%)
克隆:
Polyclonal
特异性:
Phospho-CaMK2 alpha/delta (Thr286) Antibody detects endogenous levels of CaMK2 alpha/delta only when phosphorylated at Threonine 286.
RRID:
AB_2834931
引用格式: Affinity Biosciences Cat# AF3493, RRID:AB_2834931.
偶联:
Unconjugated.
纯化:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Alpha CaMKII; Calcium calmodulin dependent protein kinase II; Calcium/calmodulin dependent protein kinase II alpha B subunit; Calcium/calmodulin dependent protein kinase type II alpha chain; Calcium/calmodulin-dependent protein kinase (CaM kinase) II alpha; Calcium/calmodulin-dependent protein kinase II alpha; Calcium/calmodulin-dependent protein kinase II-alpha; Calcium/calmodulin-dependent protein kinase type II subunit alpha; Calcium/calmodulin-dependent protein kinase type IIA; CaM kinase II alpha chain; CaM kinase II alpha subunit; CaM kinase II subunit alpha; CaMK II alpha subunit; CaMK-II subunit alpha; Camk2a; CAMKA; CaMKII; CaMKIINalpha; EC 2.7.11.17; KCC2A_HUMAN; KIAA0968; MGC123320; MGC139375; MGC155201; mKIAA0968; PK2CDD; PKCCD; R74975; zgc:112538; zgc:123320; Calcium / calmodulin dependent protein kinase 2 delta; Calcium / calmodulin dependent protein kinase II delta; calcium/calmodulin-dependent protein kinase (CaM kinase) II delta; calcium/calmodulin-dependent protein kinase type II delta chain; Calcium/calmodulin-dependent protein kinase type II subunit delta; CAM kinase 2 delta; CAM kinase II delta; CaM kinase II delta subunit; CaM kinase II subunit delta; CaM-kinase II delta chain; CAMK 2d; CaMK-II delta subunit; CaMK-II subunit delta; CAMK2D; CAMKD; CAMKI; KCC2D_HUMAN; RATCAMKI;

抗原和靶标

免疫原:
Uniprot:
基因/基因ID:
表达:
Q13557 KCC2D_HUMAN:

Expressed in cardiac muscle and skeletal muscle. Isoform Delta 3, isoform Delta 2, isoform Delta 8 and isoform Delta 9 are expressed in cardiac muscle. Isoform Delta 11 is expressed in skeletal muscle.

描述:
CaMK2-delta a protein kinase of the CAMK2 family. A prominent kinase in the central nervous system that may function in long-term potentiation and neurotransmitter release. Member of the NMDAR signaling complex in excitatory synapses that may regulate NMDAR-dependent potentiation of the AMPAR and synaptic plasticity.
序列:
MATITCTRFTEEYQLFEELGKGAFSVVRRCVKVLAGQEYAAKIINTKKLSARDHQKLEREARICRLLKHPNIVRLHDSISEEGHHYLIFDLVTGGELFEDIVAREYYSEADASHCIQQILEAVLHCHQMGVVHRDLKPENLLLASKLKGAAVKLADFGLAIEVEGEQQAWFGFAGTPGYLSPEVLRKDPYGKPVDLWACGVILYILLVGYPPFWDEDQHRLYQQIKAGAYDFPSPEWDTVTPEAKDLINKMLTINPSKRITAAEALKHPWISHRSTVASCMHRQETVDCLKKFNARRKLKGAILTTMLATRNFSGGKSGGNKKSDGVKESSESTNTTIEDEDTKVRKQEIIKVTEQLIEAISNGDFESYTKMCDPGMTAFEPEALGNLVEGLDFHRFYFENLWSRNSKPVHTTILNPHIHLMGDESACIAYIRITQYLDAGGIPRTAQSEETRVWHRRDGKWQIVHFHRSGAPSVLPH

MASTTTCTRFTDEYQLFEELGKGAFSVVRRCMKIPTGQEYAAKIINTKKLSARDHQKLEREARICRLLKHPNIVRLHDSISEEGFHYLVFDLVTGGELFEDIVAREYYSEADASHCIQQILESVNHCHLNGIVHRDLKPENLLLASKSKGAAVKLADFGLAIEVQGDQQAWFGFAGTPGYLSPEVLRKDPYGKPVDMWACGVILYILLVGYPPFWDEDQHRLYQQIKAGAYDFPSPEWDTVTPEAKDLINKMLTINPAKRITASEALKHPWICQRSTVASMMHRQETVDCLKKFNARRKLKGAILTTMLATRNFSAAKSLLKKPDGVKESTESSNTTIEDEDVKARKQEIIKVTEQLIEAINNGDFEAYTKICDPGLTAFEPEALGNLVEGMDFHRFYFENALSKSNKPIHTIILNPHVHLVGDDAACIAYIRLTQYMDGSGMPKTMQSEETRVWHRRDGKWQNVHFHRSGSPTVPIKPPCIPNGKENFSGGTSLWQNI

种属预测

种属预测:

score>80的预测可信度较高,可尝试用于WB检测。*预测模型主要基于免疫原序列比对,结果仅作参考,不作为质保凭据。

Species
Results
Score
Horse
100
Bovine
100
Sheep
100
Dog
100
Chicken
100
Rabbit
100
Xenopus
88
Zebrafish
88
Pig
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - Q9UQM7/Q13557 作为底物

Site PTM Type Enzyme
A2 Acetylation
S3 Phosphorylation
K43 Ubiquitination
K57 Ubiquitination
K69 Ubiquitination
S79 Phosphorylation
K138 Ubiquitination
S146 Phosphorylation
K227 Acetylation
K227 Ubiquitination
Y231 Phosphorylation
S235 Phosphorylation
K246 Ubiquitination
K251 Ubiquitination
T254 Phosphorylation
S264 Phosphorylation
S276 Phosphorylation
S280 Phosphorylation
T287 Phosphorylation Q13557 (CAMK2D)
K299 Methylation
K301 Methylation
T306 Phosphorylation
T307 Phosphorylation
T311 Phosphorylation
S315 Phosphorylation
K318 Acetylation
K318 Ubiquitination
S319 Phosphorylation
S330 Phosphorylation
T331 Phosphorylation
S333 Phosphorylation
S334 Phosphorylation
T336 Phosphorylation
T337 Phosphorylation
C373 S-Nitrosylation
S404 Phosphorylation
S441 Phosphorylation
S472 Phosphorylation
T474 Phosphorylation
S490 Phosphorylation
Site PTM Type Enzyme
C6 S-Nitrosylation
Y13 Phosphorylation
K56 Ubiquitination
K68 Ubiquitination
S78 Phosphorylation
K137 Ubiquitination
S145 Phosphorylation
K226 Acetylation
K226 Ubiquitination
Y230 Phosphorylation
S234 Phosphorylation
K245 Ubiquitination
T253 Phosphorylation
K258 Methylation
C280 S-Nitrosylation
T286 Phosphorylation Q9UQM7 (CAMK2A)
K298 Methylation
K300 Methylation
T305 Phosphorylation Q9UQM7 (CAMK2A)
T306 Phosphorylation Q9UQM7 (CAMK2A)
T310 Phosphorylation
S314 Phosphorylation Q9UQM7 (CAMK2A)
S318 Phosphorylation
S331 Phosphorylation
S333 Phosphorylation
T334 Phosphorylation
T336 Phosphorylation
T337 Phosphorylation

翻译修饰 - Q9UQM7/Q13557 作为激酶

Substrate Site Source
O15084 (ANKRD28) S1011 Uniprot
P08670 (VIM) S83 Uniprot
P13688-8 (CEACAM1) T457 Uniprot
P13688-8 (CEACAM1) S459 Uniprot
P16220 (CREB1) S133 Uniprot
P16220 (CREB1) S142 Uniprot
P26678 (PLN) T17 Uniprot
P55327 (TPD52) S176 Uniprot
P56524 (HDAC4) S210 Uniprot
P56524 (HDAC4) S632 Uniprot
Q00613 (HSF1) S230 Uniprot
Q03060-16 (CREM) S87 Uniprot
Q08289-2 (CACNB2) T499 Uniprot
Q08289 (CACNB2) T554 Uniprot
Q13557 (CAMK2D) T287 Uniprot
Q13557-1 (CAMK2D) S315 Uniprot
Q13557-8 (CAMK2D) S319 Uniprot
Q13557-1 (CAMK2D) T331 Uniprot
Q13557-1 (CAMK2D) S333 Uniprot
Q13557-1 (CAMK2D) S334 Uniprot
Q13557-8 (CAMK2D) T337 Uniprot
Q14524 (SCN5A) S516 Uniprot
Q14524 (SCN5A) T594 Uniprot
Q14571 (ITPR2) S150 Uniprot
Q14654 (KCNJ11) T224 Uniprot
Q16695 (HIST3H3) S11 Uniprot
Q8WYL5 (SSH1) S937 Uniprot
Q8WYL5 (SSH1) S978 Uniprot
Q8WZ42 (TTN) S4062 Uniprot
Q8WZ42 (TTN) S11878 Uniprot
Q8WZ42 (TTN) S12022 Uniprot
Q92736 (RYR2) S2814 Uniprot
Q9UQL6 (HDAC5) S498 Uniprot
Substrate Site Source
A8K161 (CAMK2A) T286 Uniprot
A8K161 (CAMK2A) T305 Uniprot
O00631 (SLN) T5 Uniprot
O14490-2 (DLGAP1) S44 Uniprot
O14713-2 (ITGB1BP1) T38 Uniprot
O15273 (TCAP) S157 Uniprot
O15273 (TCAP) S161 Uniprot
O95999 (BCL10) S138 Uniprot
P00533 (EGFR) S768 Uniprot
P00533-1 (EGFR) S1064 Uniprot
P00533 (EGFR) S1070 Uniprot
P00533 (EGFR) S1071 Uniprot
P00533-1 (EGFR) S1081 Uniprot
P00533-1 (EGFR) S1120 Uniprot
P00533 (EGFR) S1166 Uniprot
P04626 (ERBB2) T1172 Uniprot
P05556 (ITGB1) T788 Uniprot
P05556 (ITGB1) T789 Uniprot
P05783 (KRT18) S53 Uniprot
P07101 (TH) S19 Uniprot
P07101-4 (TH) S35 Uniprot
P07101-3 (TH) S40 Uniprot
P07101-4 (TH) S44 Uniprot
P07101 (TH) S71 Uniprot
P08670 (VIM) S83 Uniprot
P09917 (ALOX5) S272 Uniprot
P10636-6 (MAPT) S204 Uniprot
P10636-8 (MAPT) S262 Uniprot
P10636-2 (MAPT) S267 Uniprot
P10636-6 (MAPT) S298 Uniprot
P10636-8 (MAPT) S356 Uniprot
P10636-8 (MAPT) S416 Uniprot
P10636 (MAPT) T548 Uniprot
P10636 (MAPT) S552 Uniprot
P10636 (MAPT) S579 Uniprot
P11831 (SRF) S103 Uniprot
P11831 (SRF) T160 Uniprot
P14136-1 (GFAP) S13 Uniprot
P14136-1 (GFAP) S17 Uniprot
P14136-1 (GFAP) S38 Uniprot
P14136-1 (GFAP) S393 Uniprot
P14921 (ETS1) S251 Uniprot
P14921 (ETS1) S257 Uniprot
P14921-1 (ETS1) S282 Uniprot
P14921 (ETS1) S285 Uniprot
P15036 (ETS2) S246 Uniprot
P15036 (ETS2) S310 Uniprot
P15036 (ETS2) S313 Uniprot
P16070 (CD44) S706 Uniprot
P16220 (CREB1) S142 Uniprot
P16615-2 (ATP2A2) S38 Uniprot
P16949 (STMN1) S16 Uniprot
P17676-1 (CEBPB) S325 Uniprot
P18433 (PTPRA) S204 Uniprot
P20941-2 (PDC) S2 Uniprot
P20941-2 (PDC) S21 Uniprot
P20941-1 (PDC) S54 Uniprot
P20941-1 (PDC) S73 Uniprot
P21333 (FLNA) S2523 Uniprot
P23677 (ITPKA) T311 Uniprot
P26678 (PLN) T17 Uniprot
P28329-3 (CHAT) T456 Uniprot
P28329 (CHAT) T574 Uniprot
P29475 (NOS1) S852 Uniprot
P31645 (SLC6A4) S13 Uniprot
P35222 (CTNNB1) T332 Uniprot
P35222 (CTNNB1) T472 Uniprot
P35222 (CTNNB1) S552 Uniprot
P35270 (SPR) S213 Uniprot
P35367 (HRH1) T140 Uniprot
P35367 (HRH1) T142 Uniprot
P35367 (HRH1) S396 Uniprot
P35367 (HRH1) S398 Uniprot
P35372 (OPRM1) S268 Uniprot
P35372 (OPRM1) S270 Uniprot
P35372 (OPRM1) T372 Uniprot
P35462 (DRD3) S229 Uniprot
P37840 (SNCA) S129 Uniprot
P42261 (GRIA1) S567 Uniprot
P42261 (GRIA1) S832 Uniprot
P46527 (CDKN1B) S10 Uniprot
P48058 (GRIA4) S862 Uniprot
P51790 (CLCN3) S109 Uniprot
P55042 (RRAD) S273 Uniprot
P55055 (NR1H2) S426 Uniprot
Q00613-1 (HSF1) S230 Uniprot
Q00975 (CACNA1B) S2120 Uniprot
Q01970 (PLCB3) S537 Uniprot
Q04760 (GLO1) T107 Uniprot
Q05397 (PTK2) S843 Uniprot
Q05469 (LIPE) S855 Uniprot
Q05682 (CALD1) S643 Uniprot
Q05682 (CALD1) S656 Uniprot
Q06210 (GFPT1) S261 Uniprot
Q12879-1 (GRIN2A) S1291 Uniprot
Q12959 (DLG1) S232 Uniprot
Q13224 (GRIN2B) S383 Uniprot
Q13224 (GRIN2B) S1303 Uniprot
Q14653 (IRF3) S386 Uniprot
Q14654 (KCNJ11) T180 Uniprot
Q14654 (KCNJ11) T224 Uniprot
Q14674 (ESPL1) S1501 Uniprot
Q15121 (PEA15) S116 Uniprot
Q15796 (SMAD2) S110 Uniprot
Q15796-1 (SMAD2) S240 Uniprot
Q15796-1 (SMAD2) S260 Uniprot
Q4G163 (FBXO43) T234 Uniprot
Q86UR5 (RIMS1) S242 Uniprot
Q86UR5 (RIMS1) S288 Uniprot
Q8IZP0 (ABI1) S88 Uniprot
Q92736 (RYR2) S2808 Uniprot
Q92736 (RYR2) S2814 Uniprot
Q96A00 (PPP1R14A) S130 Uniprot
Q96NW7 (LRRC7) S1439 Uniprot
Q96PH1-4 (NOX5) S475 Uniprot
Q96PM5 (RCHY1) T154 Uniprot
Q96PM5 (RCHY1) S155 Uniprot
Q96PV0-1 (SYNGAP1) S1073 Uniprot
Q96PV0-1 (SYNGAP1) T1077 Uniprot
Q96PV0-1 (SYNGAP1) S1138 Uniprot
Q9BXL7 (CARD11) S116 Uniprot
Q9NSC5 (HOMER3) S120 Uniprot
Q9NSC5 (HOMER3) S159 Uniprot
Q9NSC5 (HOMER3) S176 Uniprot
Q9UBS5 (GABBR1) S868 Uniprot
Q9UQL6 (HDAC5) S259 Uniprot
Q9UQL6 (HDAC5) S498 Uniprot
Q9UQM7-1 (CAMK2A) T286 Uniprot
Q9UQM7-1 (CAMK2A) T305 Uniprot
Q9UQM7 (CAMK2A) T306 Uniprot
Q9UQM7 (CAMK2A) S314 Uniprot
Q9Y496 (KIF3A) S687 Uniprot
Q9Y4D2 (DAGLA) S782 Uniprot
Q9Y4D2 (DAGLA) S808 Uniprot

研究背景

功能:

Calcium/calmodulin-dependent protein kinase that functions autonomously after Ca(2+)/calmodulin-binding and autophosphorylation, and is involved in synaptic plasticity, neurotransmitter release and long-term potentiation. Member of the NMDAR signaling complex in excitatory synapses, it regulates NMDAR-dependent potentiation of the AMPAR and therefore excitatory synaptic transmission (By similarity). Regulates dendritic spine development. Also regulates the migration of developing neurons. Phosphorylates the transcription factor FOXO3 to activate its transcriptional activity.

翻译修饰:

Autophosphorylation of Thr-286 following activation by Ca(2+)/calmodulin. Phosphorylation of Thr-286 locks the kinase into an activated state.

细胞定位:

Cell junction>Synapse. Cell junction>Synapse>Postsynaptic density. Cell projection>Dendritic spine. Cell projection>Dendrite.
Note: Postsynaptic lipid rafts.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
亚基结构:

There are 4 genes encoding calcium/calmodulin-dependent protein kinase type II chains: CAMK2A, CAMK2B, CAMK2G and CAMK2D. The corresponding proteins assemble into homo- or heteromultimeric holoenzymes composed of 12 subunits with two hexameric rings stacked one on top of the other. Interacts with BAALC. Interacts with MPDZ. Interacts with SYN1. Interacts with CAMK2N2. Interacts with SYNGAP1. Interacts with SYNPO2 (By similarity). Interacts with SHANK3. Interacts with GRIN2B. Interacts with CACNB2. Interacts with LRRC7. Interacts with GRM5.

蛋白家族:

Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.

功能:

Calcium/calmodulin-dependent protein kinase involved in the regulation of Ca(2+) homeostatis and excitation-contraction coupling (ECC) in heart by targeting ion channels, transporters and accessory proteins involved in Ca(2+) influx into the myocyte, Ca(2+) release from the sarcoplasmic reticulum (SR), SR Ca(2+) uptake and Na(+) and K(+) channel transport. Targets also transcription factors and signaling molecules to regulate heart function. In its activated form, is involved in the pathogenesis of dilated cardiomyopathy and heart failure. Contributes to cardiac decompensation and heart failure by regulating SR Ca(2+) release via direct phosphorylation of RYR2 Ca(2+) channel on 'Ser-2808'. In the nucleus, phosphorylates the MEF2 repressor HDAC4, promoting its nuclear export and binding to 14-3-3 protein, and expression of MEF2 and genes involved in the hypertrophic program. Is essential for left ventricular remodeling responses to myocardial infarction. In pathological myocardial remodeling acts downstream of the beta adrenergic receptor signaling cascade to regulate key proteins involved in ECC. Regulates Ca(2+) influx to myocytes by binding and phosphorylating the L-type Ca(2+) channel subunit beta-2 CACNB2. In addition to Ca(2+) channels, can target and regulate the cardiac sarcolemmal Na(+) channel Nav1.5/SCN5A and the K+ channel Kv4.3/KCND3, which contribute to arrhythmogenesis in heart failure. Phosphorylates phospholamban (PLN/PLB), an endogenous inhibitor of SERCA2A/ATP2A2, contributing to the enhancement of SR Ca(2+) uptake that may be important in frequency-dependent acceleration of relaxation (FDAR) and maintenance of contractile function during acidosis. May participate in the modulation of skeletal muscle function in response to exercise, by regulating SR Ca(2+) transport through phosphorylation of PLN/PLB and triadin, a ryanodine receptor-coupling factor.

翻译修饰:

Autophosphorylation of Thr-287 following activation by Ca(2+)/calmodulin. Phosphorylation of Thr-287 locks the kinase into an activated state.

细胞定位:

Cell membrane>Sarcolemma>Peripheral membrane protein>Cytoplasmic side. Sarcoplasmic reticulum membrane>Peripheral membrane protein>Cytoplasmic side.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Expressed in cardiac muscle and skeletal muscle. Isoform Delta 3, isoform Delta 2, isoform Delta 8 and isoform Delta 9 are expressed in cardiac muscle. Isoform Delta 11 is expressed in skeletal muscle.

亚基结构:

CAMK2 is composed of 4 different chains: alpha (CAMK2A), beta (CAMK2B), gamma (CAMK2G), and delta (CAMK2D). The different isoforms assemble into homo- or heteromultimeric holoenzymes composed of 12 subunits with two hexameric rings stacked one on top of the other. Interacts with RRAD and CACNB2 (By similarity).

蛋白家族:

The CAMK2 protein kinases contain a unique C-terminal subunit association domain responsible for oligomerization.

Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.

研究领域

· Cellular Processes > Cell growth and death > Oocyte meiosis.   (View pathway)

· Cellular Processes > Cell growth and death > Necroptosis.   (View pathway)

· Environmental Information Processing > Signal transduction > ErbB signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Calcium signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > cAMP signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > HIF-1 signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Wnt signaling pathway.   (View pathway)

· Human Diseases > Substance dependence > Amphetamine addiction.

· Human Diseases > Infectious diseases: Bacterial > Tuberculosis.

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Human Diseases > Cancers: Overview > Proteoglycans in cancer.

· Human Diseases > Cancers: Specific types > Glioma.   (View pathway)

· Organismal Systems > Circulatory system > Adrenergic signaling in cardiomyocytes.   (View pathway)

· Organismal Systems > Development > Axon guidance.   (View pathway)

· Organismal Systems > Environmental adaptation > Circadian entrainment.

· Organismal Systems > Nervous system > Long-term potentiation.

· Organismal Systems > Nervous system > Neurotrophin signaling pathway.   (View pathway)

· Organismal Systems > Nervous system > Cholinergic synapse.

· Organismal Systems > Nervous system > Dopaminergic synapse.

· Organismal Systems > Sensory system > Olfactory transduction.

· Organismal Systems > Sensory system > Inflammatory mediator regulation of TRP channels.   (View pathway)

· Organismal Systems > Endocrine system > Insulin secretion.   (View pathway)

· Organismal Systems > Endocrine system > Melanogenesis.

· Organismal Systems > Endocrine system > Oxytocin signaling pathway.

· Organismal Systems > Endocrine system > Glucagon signaling pathway.

· Organismal Systems > Endocrine system > Aldosterone synthesis and secretion.

· Organismal Systems > Digestive system > Gastric acid secretion.

文献引用

1). Overproduction of Gastrointestinal 5-HT Promotes Colitis-Associated Colorectal Cancer Progression via Enhancing NLRP3 Inflammasome Activation. Cancer Immunology Research (PubMed: 34285037) [IF=10.1]

2). lncRNA-ZFAS1 induces mitochondria-mediated apoptosis by causing cytosolic Ca2+ overload in myocardial infarction mice model. Cell Death & Disease (PubMed: 31819041) [IF=9.0]

Application: WB    Species: Mice    Sample: Cardiac tissue

As shown in Fig. S6, ZFAS1 had no obvious effects on the expression of P-CaMKII, CaMKII, or Cx43 protein levels. The calcium homeostasis of the TG was also assessed by optical-mapping techniques.

3). Antiarrhythmic effects of ginsenoside Rg2 on calcium chloride–induced arrhythmias without oral toxicity. Journal of Ginseng Research (PubMed: 32913401) [IF=6.3]

4). Role of Ion Channels in Chronic Intermittent Hypoxia-Induced Atrial Remodeling in Rats. LIFE SCIENCES (PubMed: 32417371) [IF=6.1]

Application: WB    Species: rat    Sample: atrial

Fig. 6.| Protein levels of Nav1.5, Cav1.2, Kv4.2, Kv4.3, Kv1.5, NCX1, RyR2, p-RyR2, CaMKII, p-CaMKII confirmed by Western Blotting in two groups (n = 10). And p-RyR2/RyR2, p-CaMKII/CaMKII were also calculated and compared between two groups. LA -left atrial, RA -right atrial.

5). Evodiamine induces ROS-Dependent cytotoxicity in human gastric cancer cells via TRPV1/Ca2+ pathway. CHEMICO-BIOLOGICAL INTERACTIONS (PubMed: 34808100) [IF=5.1]

6). Dezocine attenuates the remifentanil-induced postoperative hyperalgesia by inhibition of phosphorylation of CaMKⅡα. European Journal of Pharmacology (PubMed: 31863769) [IF=5.0]

Application: WB    Species: Mice    Sample: spinal cord

Fig. 4. Suppression of Remifentanil-induced CaMKⅡα activation by dezocine in the spinal cord. (A) Western blot analysis showed that Remifentanil (40μg/kg) enchanced p-CaMKⅡα activation in spinal cord, without altering total CaMKⅡα expression. Dezocine dose-dependently prevented remifentanil enhanced p-CaMKⅡα activation. Ketamine also decreased remifentanil enhanced p-CaMKⅡα activation in spinal cord. (B) Bar plot summary of p-CaMKⅡα in spinal cord. n = 3 for each group. (C) Remifentanil elevated immunohistochemical staining of p-CaMKⅡα in spinal dorsal horn. Dezocine dose-dependently prevented elevated p-CaMKⅡα immunoreactivity in spinal dorsal horn. Ketamine also decreased p-CaMKⅡα immunoreactivity in spinal dorsal horn. Scale bars: 50 μm (left), 20 μm (right). (D) Quantification of p-CaMKⅡα immunoreactivity in spinal dorsal horn. n = 2 for each group. Final data are expressed as the mean ± S.E.M. One-way ANOVA followed by the Tukey test was performed. **P < 0.01, *P < 0.05

7). Cobratoxin Alleviates Cancer-Induced Bone Pain in Rats via Inhibiting CaMKII Signaling Pathway after Acting on M4 Muscarinic Cholinergic Receptors. ACS Chemical Neuroscience (PubMed: 35420768) [IF=5.0]

8). Sinomenine Protects Against Morphine Dependence through the NMDAR1/CAMKII/CREB Pathway: A Possible Role of Astrocyte-Derived Exosomes. MOLECULES (PubMed: 30227624) [IF=4.6]

Application: WB    Species: mouse    Sample: SH-SY5Y cells

Figure 6. |Effects of astrocyte-derived exosomes on morphine-treated SH-SY5Y cells and subsequent intervention by sinomenine.(F–H) NMDAR agonist reversed the inhibitory effect of Sino-exo on p-NMDAR1/NMDAR1, p-CAMKII/CAMKII, and p-CREB/CREB (n = 3). Ctl-exo:exosomes extracted from cultured primary astrocytes; mor-exo: exosomes extracted from cultured primary astrocytes treated with 100 µM morphine for 48 h; Sino-exo: exosomes extracted from cultured primary astrocytes treated with 100 µM morphine and 200 µMsinomenine for 48 h. # p < 0.05, ## p < 0.01 vs. ctl-exo; ∆ p < 0.05, ∆∆ p < 0.01 vs. mor-exo; * p < 0.05, ** p < 0.01 vs. Sino-exo.

9). Cypermethrin induces Sertoli cell apoptosis through mitochondrial pathway associated with calcium. Toxicology Research (PubMed: 34484665) [IF=2.1]

Application: WB    Species: Mice    Sample: TM4 cells

Figure 4 Effects of cypermethrin on Ca2+/CaM/CaMKII pathway. TM4 cells were treated with 0 μM, 10 μM, 20 μM, 40 μM and 80 μM of cypermethrin. (A) Protein expression of CaM. (B) Protein expressions of phosphorylated CaMKII and CaMKII. Data were expressed as means ±SD (n = 3). *P < 0.05 compared with control group.

10). Calcium Channel Blocker Nifedipine Suppresses Colorectal Cancer Progression and Immune Escape by Preventing NFAT2 Nuclear Translocation.

Application: WB    Species: Human    Sample: SW620 cells

Figure 3. The Calcium-Dependent Gene NFAT2 Is Activated through Dephosphorylation and Transportation to the Nucleus (A) IF assay was used to visualize the alteration of NFAT2 in SW620 cells treated with NIFE (10 mM) or the NFAT2 vector. (B) IF assay was used to visualize the alteration in NFAT2 in SW620 cells treated with NFAT2 short hairpin RNA (shRNA) or BAY (10 mM). (C) IF assays were performed with subcutaneous tumor slices from mice treated with NIFE or BAY to visualize the subcellular localization of NFAT2. (D) After treating SW620 cells with NIFE or BAY, IF assays were used to visualize the subcellular localization of NFAT2 and phosphorylated NFAT2 (p-NFAT2). (E) IF assay was used to visualize the Ca2+ level and NFAT2 or p-NFAT2 level in normal tissues and tumors. Images in the same line were from the same magnification. Blue light represents the basic light of Ca2+, whereas green light represents the elevated Ca2+ levels. Red light represents the level of NFAT2. (F) Upper panel: detection of total protein changes in SW620 cells after treatment with NIFE or transfection with siRNA-CAMK-II by WB. Lower panel: proteins contained in cytoplasmic and nuclear compartments of SW620 cells after treatment with NIFE or BAY were separated for WB analysis. (G) After treating SW620 cells with CsA (10 mM), VIVIT (10 mM), and FK506 (10 ng/mL) and subsequently separating the nuclear and cytoplasmic proteins, WB analysis was used to detect the level of NFAT2 in the nucleus and p-NFAT2 in the cytoplasm. (H) Representative images of IHC staining analysis of NFAT2 expression in CRC tissues and adjacent nontumor tissues. The bar chart on the right represents the percentage of high and low NFAT2 expression cases in normal and CRC tissues. (I) IHC analysis of NFAT2 and p-NFAT2 expression in nonmetastatic and metastatic CRC tissues. The bar chart on the right represents the percentage of high and low NFAT2 or p-NFAT2 expression cases in nonmetastatic CRC and metastatic CRC tissues.

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