产品: TNNI3 抗体
货号: AF6214
描述: Rabbit polyclonal antibody to TNNI3
应用: WB IHC
反应: Human, Mouse, Rat, Monkey
预测: Pig, Bovine, Horse, Sheep, Rabbit, Dog
分子量: 28kDa; 24kD(Calculated).
蛋白号: P19429
RRID: AB_2835095

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat,Monkey
预测:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(93%), Dog(100%)
克隆:
Polyclonal
特异性:
TNNI3 Antibody detects endogenous levels of total TNNI3.
RRID:
AB_2835095
引用格式: Affinity Biosciences Cat# AF6214, RRID:AB_2835095.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

cardiac muscle; Cardiac troponin I; cardiomyopathy, dilated 2A (autosomal recessive); Cardiomyopathy, familial hypertrophic, 7, included; CMD1FF; CMD2A; CMH7; cTnI; Familial hypertrophic cardiomyopathy 7; MGC116817; RCM1; Tn1; Tni; TNN I3; TNNC 1; TNNC1; TNNI3; TNNI3_HUMAN; Troponin I; Troponin I cardiac; Troponin I cardiac muscle; Troponin I cardiac muscle isoform; Troponin I type 3 cardiac; troponin I, cardiac 3; TroponinI; Ttroponin I type 3 (cardiac);

抗原和靶标

免疫原:
Uniprot:
基因/基因ID:
描述:
Troponin I (TnI) is one of 3 subunits that form the troponin complex of the thin filaments of striated muscle; see also TNNI1 (MIM 191042). The others are troponin T (TnT; see MIM 191041) and troponin C (TnC; see MIM 191040).
序列:
MADGSSDAAREPRPAPAPIRRRSSNYRAYATEPHAKKKSKISASRKLQLKTLLLQIAKQELEREAEERRGEKGRALSTRCQPLELAGLGFAELQDLCRQLHARVDKVDEERYDIEAKVTKNITEIADLTQKIFDLRGKFKRPTLRRVRISADAMMQALLGARAKESLDLRAHLKQVKKEDTEKENREVGDWRKNIDALSGMEGRKKKFES

种属预测

种属预测:

score>80的预测可信度较高,可尝试用于WB检测。*预测模型主要基于免疫原序列比对,结果仅作参考,不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
93
Xenopus
77
Chicken
69
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P19429 作为底物

Site PTM Type Enzyme
A2 Acetylation
S5 Phosphorylation
S6 Phosphorylation
S23 Phosphorylation Q13976 (PRKG1) , Q15139 (PRKD1) , P17612 (PRKACA) , Q05655 (PRKCD) , Q13131 (PRKAA1) , P17252 (PRKCA)
S24 Phosphorylation Q05655 (PRKCD) , P17612 (PRKACA) , P17252 (PRKCA) , Q15139 (PRKD1) , Q13976 (PRKG1) , Q13131 (PRKAA1)
Y26 Phosphorylation
Y29 Phosphorylation
T31 Phosphorylation Q13043 (STK4)
S39 Phosphorylation P17612 (PRKACA)
K40 Methylation
S42 Phosphorylation P17252 (PRKCA) , P05129 (PRKCG) , P05771 (PRKCB)
S44 Phosphorylation P05771 (PRKCB) , P17252 (PRKCA) , Q02156 (PRKCE) , P05129 (PRKCG)
T51 Phosphorylation Q13043 (STK4)
K72 Acetylation
S77 Phosphorylation P17252 (PRKCA)
T78 Phosphorylation
Y112 Phosphorylation
T129 Phosphorylation Q13043 (STK4)
T143 Phosphorylation Q05655 (PRKCD) , Q13043 (STK4) , P05771 (PRKCB) , P17252 (PRKCA)
S150 Phosphorylation O75914 (PAK3) , Q13131 (PRKAA1)
S166 Phosphorylation P17612 (PRKACA)
K177 Acetylation
K178 Acetylation
T181 Phosphorylation
S199 Phosphorylation P17252 (PRKCA)

研究背景

功能:

Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.

翻译修饰:

Phosphorylated at Ser-42 and Ser-44 by PRKCE; phosphorylation increases myocardium contractile dysfunction (By similarity). Phosphorylated at Ser-23 and Ser-24 by PRKD1; phosphorylation reduces myofilament calcium sensitivity. Phosphorylated preferentially at Thr-31. Phosphorylation by STK4/MST1 alters its binding affinity to TNNC1 (cardiac Tn-C) and TNNT2 (cardiac Tn-T).

亚基结构:

Binds to actin and tropomyosin. Interacts with TRIM63. Interacts with STK4/MST1.

蛋白家族:

Belongs to the troponin I family.

研究领域

· Environmental Information Processing > Signal transduction > cAMP signaling pathway.   (View pathway)

· Human Diseases > Cardiovascular diseases > Hypertrophic cardiomyopathy (HCM).

· Human Diseases > Cardiovascular diseases > Dilated cardiomyopathy (DCM).

· Organismal Systems > Circulatory system > Cardiac muscle contraction.   (View pathway)

· Organismal Systems > Circulatory system > Adrenergic signaling in cardiomyocytes.   (View pathway)

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