UBE1L Antibody - #AF0288

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产品: UBE1L 抗体
货号: AF0288
描述: Rabbit polyclonal antibody to UBE1L
应用: WB IHC IF/ICC
反应: Human
预测: Pig, Rabbit
分子量: 112kDa; 112kD(Calculated).
蛋白号: P41226
RRID: AB_2833456

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:3000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human
预测:
Pig(85%), Rabbit(85%)
克隆:
Polyclonal
特异性:
UBE1L Antibody detects endogenous levels of total UBE1L.
RRID:
AB_2833456
引用格式: Affinity Biosciences Cat# AF0288, RRID:AB_2833456.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

D8; MGC12713; UBA1, ubiquitin activating enzyme E1 homolog B; UBA1B; UBA7; UBA7, ubiquitin activating enzyme E1; UBA7_HUMAN; Ube 1L; UBE 2; UBE1L; UBE2; Ubiquitin activating enzyme 2; Ubiquitin activating enzyme E1 homolog; Ubiquitin Activating Enzyme E1 Like; Ubiquitin activating enzyme E1 related protein; Ubiquitin-activating enzyme 7; Ubiquitin-activating enzyme E1 homolog; ubiquitin-activating enzyme-2; ubiquitin-like modifier activating enzyme 7; Ubiquitin-like modifier-activating enzyme 7;

抗原和靶标

免疫原:
Uniprot:

P41226(UBA7_HUMAN) >>Visit HPA database.

基因/基因ID:
UBA7(7318)
表达:
P41226 UBA7_HUMAN:

Expressed in a variety of normal and tumor cell types, but is reduced in lung cancer cell lines.

描述:
UBE1L Activates ubiquitin by first adenylating with ATP its C- terminal glycine residue and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding an ubiquitin- E1 thioester and free AMP. Belongs to the ubiquitin-activating E1 family.
序列:
MDALDASKLLDEELYSRQLYVLGSPAMQRIQGARVLVSGLQGLGAEVAKNLVLMGVGSLTLHDPHPTCWSDLAAQFLLSEQDLERSRAEASQELLAQLNRAVQVVVHTGDITEDLLLDFQVVVLTAAKLEEQLKVGTLCHKHGVCFLAADTRGLVGQLFCDFGEDFTVQDPTEAEPLTAAIQHISQGSPGILTLRKGANTHYFRDGDLVTFSGIEGMVELNDCDPRSIHVREDGSLEIGDTTTFSRYLRGGAITEVKRPKTVRHKSLDTALLQPHVVAQSSQEVHHAHCLHQAFCALHKFQHLHGRPPQPWDPVDAETVVGLARDLEPLKRTEEEPLEEPLDEALVRTVALSSAGVLSPMVAMLGAVAAQEVLKAISRKFMPLDQWLYFDALDCLPEDGELLPSPEDCALRGSRYDGQIAVFGAGFQEKLRRQHYLLVGAGAIGCELLKVFALVGLGAGNSGGLTVVDMDHIERSNLSRQFLFRSQDVGRPKAEVAAAAARGLNPDLQVIPLTYPLDPTTEHIYGDNFFSRVDGVAAALDSFQARRYVAARCTHYLKPLLEAGTSGTWGSATVFMPHVTEAYRAPASAAASEDAPYPVCTVRYFPSTAEHTLQWARHEFEELFRLSAETINHHQQAHTSLADMDEPQTLTLLKPVLGVLRVRPQNWQDCVAWALGHWKLCFHYGIKQLLRHFPPNKVLEDGTPFWSGPKQCPQPLEFDTNQDTHLLYVLAAANLYAQMHGLPGSQDWTALRELLKLLPQPDPQQMAPIFASNLELASASAEFGPEQQKELNKALEVWSVGPPLKPLMFEKDDDSNFHVDFVVAAASLRCQNYGIPPVNRAQSKRIVGQIIPAIATTTAAVAGLLGLELYKVVSGPRPRSAFRHSYLHLAENYLIRYMPFAPAIQTFHHLKWTSWDRLKVPAGQPERTLESLLAHLQEQHGLRVRILLHGSALLYAAGWSPEKQAQHLPLRVTELVQQLTGQAPAPGQRVLVLELSCEGDDEDTAFPPLHYEL

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Rabbit
85
Pig
85
Horse
77
Dog
73
Bovine
62
Chicken
55
Sheep
0
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P41226 作为底物

Site PTM Type Enzyme
K8 Ubiquitination
Y15 Phosphorylation
S24 Phosphorylation
K134 Ubiquitination
K257 Ubiquitination
S266 Phosphorylation
K330 Ubiquitination
K429 Ubiquitination
S485 Phosphorylation
K492 Ubiquitination
Y514 Phosphorylation
Y524 Phosphorylation
Y555 Phosphorylation
K696 Ubiquitination
S879 Phosphorylation
S884 Phosphorylation
Y892 Phosphorylation
Y896 Phosphorylation

研究背景

功能:

Activates ubiquitin by first adenylating with ATP its C-terminal glycine residue and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding a ubiquitin-E1 thioester and free AMP. Catalyzes the ISGylation of influenza A virus NS1 protein.

翻译修饰:

ISGylated.

组织特异性:

Expressed in a variety of normal and tumor cell types, but is reduced in lung cancer cell lines.

亚基结构:

Monomer (By similarity). Binds and is involved in the conjugation of G1P2/ISG15.

蛋白家族:

Belongs to the ubiquitin-activating E1 family.

研究领域

· Genetic Information Processing > Folding, sorting and degradation > Ubiquitin mediated proteolysis.   (View pathway)

· Human Diseases > Neurodegenerative diseases > Parkinson's disease.

文献引用

1). Heme oxygenase-1 prevents heart against myocardial infarction by attenuating ischemic injury-induced cardiomyocytes senescence. EBioMedicine (PubMed: 30527623) [IF=11.1]

Application: WB    Species: mouse    Sample: cardiomyocytes

Fig. 4.| HO-1 repressed cardiomyocytes senescence under the treatment of H2O2 in vitro. Primary cardiomyocytes were treated with H2O2 (50 μM) for 3 h and hemin (10 μM) for 24 h.(a) The expression levels of LaminB, p53 and p16 analyzed by western blot. n = 3. *P b .01, **P b .05. Data are mean ± SEM; one-way ANOVA was used for the statistical analysis.(b) and (c) SA-β-gal activity analyzed by Senescence β-Galactosidase Staining Kit. Scale bars represent 100 μm n = 4. **P b .05. Data are mean ± SEM; Two-tailed t-test was used for the statistical analysis.
2). HSP90AA1 interacts with CSFV NS5A protein and regulates CSFV replication via the JAK/STAT and NF-κB signaling pathway. Frontiers in Immunology (PubMed: 36405689) [IF=7.3]

Application: WB    Species: pig    Sample: PK-15 cell

Figure 8 CSFV infection antagonizes the activation of HSP90AA1 on JAK/STAT pathway. (A, B) Western blot for JAK1, STAT1, p-JAK1, p-STAT1, OAS2, ISG-15 and HSP90AA1 expression in HSP90AA1-overexpression or knock-downed PK-15 and 3D4/2 cells. Cells were infected with CSFV (MOI=1) after transfection p3×Flag-HSP90AA1 or siHSP90AA1. The cells were not infected with CSFV as a control. Cells were harvested at 24hpi and 48hpi served to Western blot. The relative levels of proteins were estimated by histograms representing density reading of the gel bands with Image J, and the ratios were calculated relative to tubulin control. (*p < 0.05, **p < 0.01, ***p < 0.001 and ****p < 0.0001 calculated using two-way ANOVA, ns, not significant).

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