DHRS4 Antibody - #DF3986

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产品: DHRS4 抗体
货号: DF3986
描述: Rabbit polyclonal antibody to DHRS4
应用: WB IHC IF/ICC
反应: Human, Mouse, Rat
分子量: 32-34 KD; 30kD(Calculated).
蛋白号: Q9BTZ2
RRID: AB_2836346

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 50ul RMB¥ 1250 现货
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产品描述

来源:
Rabbit
应用:
WB 1:500-1:1000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
克隆:
Polyclonal
特异性:
DHRS4 Antibody detects endogenous levels of total DHRS4.
RRID:
AB_2836346
引用格式: Affinity Biosciences Cat# DF3986, RRID:AB_2836346.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

AI043103; AI790593; Carbonyl reductase; CR; D14Ucla2; Dehydrogenase/reductase SDR family member 4; Dhrs4; DHRS4_HUMAN; humNRDR; mouNRDR; NADPH dependent carbonyl reductase/NADP retinol dehydrogenase; NADPH dependent retinol dehydrogenase/reductase; NADPH-dependent carbonyl reductase/NADP-retinol dehydrogenase; NADPH-dependent retinol dehydrogenase/reductase; NDRD; NRDR; Peroxisomal short chain alcohol dehydrogenase; Peroxisomal short-chain alcohol dehydrogenase; PHCR; PRO1800; PSCD; RRD; SCAD-SRL; SCADSRL; Short chain dehydrogenase/reductase family member 4; Short-chain dehydrogenase/reductase family member 4; UNQ851;

抗原和靶标

免疫原:
Uniprot:

Q9BTZ2(DHRS4_HUMAN) >>Visit HPA database.

基因/基因ID:
DHRS4(10901)
表达:
Q9BTZ2 DHRS4_HUMAN:

Isoform 1 is predominantly expressed in normal cervix (at protein level). Isoform 4 is expressed in some neoplastic cervical tissues, but not in normal cervix (at protein level). Isoform 5 and isoform 6 are expressed in a few neoplastic cervical tissues.

序列:
MHKAGLLGLCARAWNSVRMASSGMTRRDPLANKVALVTASTDGIGFAIARRLAQDGAHVVVSSRKQQNVDQAVATLQGEGLSVTGTVCHVGKAEDRERLVATAVKLHGGIDILVSNAAVNPFFGSIMDVTEEVWDKTLDINVKAPALMTKAVVPEMEKRGGGSVVIVSSIAAFSPSPGFSPYNVSKTALLGLTKTLAIELAPRNIRVNCLAPGLIKTSFSRMLWMDKEKEESMKETLRIRRLGEPEDCAGIVSFLCSEDASYITGETVVVGGGTPSRL

翻译修饰 - Q9BTZ2 作为底物

Site PTM Type Enzyme
S63 Phosphorylation
K65 Ubiquitination
T149 Phosphorylation
K150 Ubiquitination
K194 Ubiquitination
K216 Ubiquitination
T274 Phosphorylation

研究背景

功能:

Reduces all-trans-retinal and 9-cis retinal. Can also catalyze the oxidation of all-trans-retinol with NADP as co-factor, but with much lower efficiency. Reduces alkyl phenyl ketones and alpha-dicarbonyl compounds with aromatic rings, such as pyrimidine-4-aldehyde, 3-benzoylpyridine, 4-benzoylpyridine, menadione and 4-hexanoylpyridine. Has no activity towards aliphatic aldehydes and ketones (By similarity).

细胞定位:

Peroxisome.
Note: Isoform 1 is peroxisomal, while isoform 4 is not.

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Isoform 1 is predominantly expressed in normal cervix (at protein level). Isoform 4 is expressed in some neoplastic cervical tissues, but not in normal cervix (at protein level). Isoform 5 and isoform 6 are expressed in a few neoplastic cervical tissues.

亚基结构:

Homotetramer.

蛋白家族:

Belongs to the short-chain dehydrogenases/reductases (SDR) family.

研究领域

· Cellular Processes > Transport and catabolism > Peroxisome.   (View pathway)

· Metabolism > Metabolism of cofactors and vitamins > Retinol metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

文献引用

1). Identification of Molecular Correlations Between DHRS4 and Progressive Neurodegeneration in Amyotrophic Lateral Sclerosis By Gene Co-Expression Network Analysis. Frontiers in Immunology (PubMed: 35479082) [IF=7.3]

Application: WB    Species: Mice    Sample: immune cells

Figure 4 Validation of Dhrs4 by Western blotting and immunofluorescence. (A–C) Representative Western blotting images of Dhrs4 in SOD1G93A mice compared with WT mice. (A, B) Dhrs4 expression has no significant difference in pre-onset and onset stages, respectively. (C) At progression stage of SOD1G93A mice, the expression of Dhrs4 is markedly upregulated. Data are presented as the mean ± SD (n = 3 or 4 per group), unpaired t-test, ***p < 0.001. (D) The expression of Dhrs4 in neurons is shown. The NeuN and Dhrs4 are double-labeled performed with anti-Dhrs4 (red) and anti-NeuN (green), respectively. (E) Quantifications of Dhrs4 fluorescence intensity per neuron (IOD). Data are presented as the mean ± SD (n = 3 per group), unpaired t-test with Welch’s correction; ***p < 0.001. Scale bars, 20 μm. WT, wild type. IOD, Integral optical density.

Application: IF/ICC    Species: Mice    Sample: immune cells

Figure 4 Validation of Dhrs4 by Western blotting and immunofluorescence. (A–C) Representative Western blotting images of Dhrs4 in SOD1G93A mice compared with WT mice. (A, B) Dhrs4 expression has no significant difference in pre-onset and onset stages, respectively. (C) At progression stage of SOD1G93A mice, the expression of Dhrs4 is markedly upregulated. Data are presented as the mean ± SD (n = 3 or 4 per group), unpaired t-test, ***p < 0.001. (D) The expression of Dhrs4 in neurons is shown. The NeuN and Dhrs4 are double-labeled performed with anti-Dhrs4 (red) and anti-NeuN (green), respectively. (E) Quantifications of Dhrs4 fluorescence intensity per neuron (IOD). Data are presented as the mean ± SD (n = 3 per group), unpaired t-test with Welch’s correction; ***p < 0.001. Scale bars, 20 μm. WT, wild type. IOD, Integral optical density.

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