CD168 Antibody - #DF4809

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产品: CD168 抗体
货号: DF4809
描述: Rabbit polyclonal antibody to CD168
应用: WB IHC IF/ICC
反应: Human
预测: Pig, Horse, Dog
分子量: 85 KD; 84kD(Calculated).
蛋白号: O75330
RRID: AB_2837160

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

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实验方案
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产品描述

来源:
Rabbit
应用:
WB 1:500-1:1000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human
预测:
Pig(80%), Horse(80%), Dog(80%)
克隆:
Polyclonal
特异性:
CD168 Antibody detects endogenous levels of total CD168.
RRID:
AB_2837160
引用格式: Affinity Biosciences Cat# DF4809, RRID:AB_2837160.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

CD168; CD168 antigen; HMMR; HMMR_HUMAN; Hyaluronan mediated motility receptor; Hyaluronan-mediated motility receptor (RHAMM); IHABP; Intracellular hyaluronic acid-binding protein; MGC119494; MGC119495; OTTHUMP00000196920; Receptor for hyaluronan-mediated motility; RHAMM;

抗原和靶标

免疫原:
Uniprot:

O75330(HMMR_HUMAN) >>Visit HPA database.

基因/基因ID:
HMMR(3161)
表达:
O75330 HMMR_HUMAN:

Expressed in breast cancer cell lines and in normal breast tissue.

序列:
MSFPKAPLKRFNDPSGCAPSPGAYDVKTLEVLKGPVSFQKSQRFKQQKESKQNLNVDKDTTLPASARKVKSSESKESQKNDKDLKILEKEIRVLLQERGAQDRRIQDLETELEKMEARLNAALREKTSLSANNATLEKQLIELTRTNELLKSKFSENGNQKNLRILSLELMKLRNKRETKMRGMMAKQEGMEMKLQVTQRSLEESQGKIAQLEGKLVSIEKEKIDEKSETEKLLEYIEEISCASDQVEKYKLDIAQLEENLKEKNDEILSLKQSLEENIVILSKQVEDLNVKCQLLEKEKEDHVNRNREHNENLNAEMQNLKQKFILEQQEREKLQQKELQIDSLLQQEKELSSSLHQKLCSFQEEMVKEKNLFEEELKQTLDELDKLQQKEEQAERLVKQLEEEAKSRAEELKLLEEKLKGKEAELEKSSAAHTQATLLLQEKYDSMVQSLEDVTAQFESYKALTASEIEDLKLENSSLQEKAAKAGKNAEDVQHQILATESSNQEYVRMLLDLQTKSALKETEIKEITVSFLQKITDLQNQLKQQEEDFRKQLEDEEGRKAEKENTTAELTEEINKWRLLYEELYNKTKPFQLQLDAFEVEKQALLNEHGAAQEQLNKIRDSYAKLLGHQNLKQKIKHVVKLKDENSQLKSEVSKLRCQLAKKKQSETKLQEELNKVLGIKHFDPSKAFHHESKENFALKTPLKEGNTNCYRAPMECQESWK

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
80
Horse
80
Dog
80
Bovine
78
Sheep
67
Xenopus
60
Rabbit
60
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - O75330 作为底物

Site PTM Type Enzyme
S2 Phosphorylation
S20 Phosphorylation
S41 Phosphorylation
S65 Phosphorylation
T110 Phosphorylation
K114 Ubiquitination
K138 Ubiquitination
T198 Phosphorylation
S205 Phosphorylation
K215 Ubiquitination
K221 Ubiquitination
Y250 Phosphorylation
K251 Ubiquitination
K338 Ubiquitination
K350 Ubiquitination
S353 Phosphorylation
K419 Ubiquitination
K489 Ubiquitination
K604 Ubiquitination
K635 Ubiquitination
S656 Phosphorylation
K696 Ubiquitination
T703 Phosphorylation
Y713 Phosphorylation

研究背景

功能:

Receptor for hyaluronic acid (HA) (By similarity). Involved in cell motility (By similarity). When hyaluronan binds to HMMR, the phosphorylation of a number of proteins, including PTK2/FAK1 occurs. May also be involved in cellular transformation and metastasis formation, and in regulating extracellular-regulated kinase (ERK) activity. May act as a regulator of adipogenisis (By similarity).

细胞定位:

Cell surface. Cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Expressed in breast cancer cell lines and in normal breast tissue.

亚基结构:

Subunit of the HARC complex. Interacts with ANKRD26.

研究领域

· Environmental Information Processing > Signaling molecules and interaction > ECM-receptor interaction.   (View pathway)

文献引用

1). The critical role of B4GALT4 in promoting microtubule spindle assembly in HCC through the regulation of PLK1 and RHAMM expression. JOURNAL OF CELLULAR PHYSIOLOGY (PubMed: 34270095) [IF=5.6]

Application: WB    Species: Human    Sample: HepG2 cells

FIGURE 9 B4GALT4 knockdown regulated TGF‐beta1/TGF‐beta receptor‐1 signaling pathway and modified the expressions of PLK1 and RHAMM. (a) B4GALT4 knockdown downregulated the expression of lumican. (b) The expressions of B4GALT4, P‐Smad2, Smad2, PLK1, and RHAMM in HepG2 cells after different treatments as mentioned above were detected by Western blot analysis. (c) The comparison of the mRNA expression level of HMMR between tumor and normal samples. (d) Survival analysis of HMMR in HCC. (e) The protein interaction network diagram of RHAMM, PLK1, and other pathway‐related genes. (f) ChIP‐seq results showed that Smad2/3 and p300 binding regions are enriched around the promoter of PLK1. (g) Schematic illustration of the B4GALT4‐mediated regulatory network responsible for the microtubule spindle assembly in HCC (****p<0.0001). B4GALT, beta 1,4‐galactosyltransferase; HCC, hepatocellular carcinoma; mRNA, messenger RNA

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