ADLD; lamin B1; Lamin-B1; LMN; LMN2; LMNB; Lmnb1; LMNB1_HUMAN; MGC111419; OTTHUMP00000159218;
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:200, ELISA(peptide) 1:20000-1:40000
*The optimal dilutions should be determined by the end user.
Human, Mouse, Rat, Monkey, Fish
Pig(100%), Bovine(100%), Sheep(100%), Rabbit(100%), Xenopus(83%)
Rabbit
Polyclonal
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Lamin B1 Antibody detects endogenous levels of total Lamin B.
A synthesized peptide derived from human Lamin B1.
AB_2837647
引用格式: Affinity Biosciences Cat# AF5161, RRID:AB_2837647.
Liquid
1mg/ml
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
A synthesized peptide derived from human Lamin B1.
>>Visit The Human Protein Atlas
LMNB1
Observed Mol.Wt.: 66kD.
Predicted Mol.Wt.: 66kDa(Calculated)..
Nucleus inner membrane.
Lamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin.
MATATPVPPRMGSRAGGPTTPLSPTRLSRLQEKEELRELNDRLAVYIDKVRSLETENSALQLQVTEREEVRGRELTGLKALYETELADARRALDDTARERAKLQIELGKCKAEHDQLLLNYAKKESDLNGAQIKLREYEAALNSKDAALATALGDKKSLEGDLEDLKDQIAQLEASLAAAKKQLADETLLKVDLENRCQSLTEDLEFRKSMYEEEINETRRKHETRLVEVDSGRQIEYEYKLAQALHEMREQHDAQVRLYKEELEQTYHAKLENARLSSEMNTSTVNSAREELMESRMRIESLSSQLSNLQKESRACLERIQELEDLLAKEKDNSRRMLTDKEREMAEIRDQMQQQLNDYEQLLDVKLALDMEISAYRKLLEGEEERLKLSPSPSSRVTVSRASSSRSVRTTRGKRKRVDVEESEASSSVSISHSASATGNVCIEEIDVDGKFIRLKNTSEQDQPMGGWEMIRKIGDTSVSYKYTSRYVLKAGQTVTIWAANAGVTASPPTDLIWKNQNSWGTGEDVKVILKNSQGEEVAQRSTVFKTTIPEEEEEEEEAAGVVVEEELFHQQGTPRASNRSCAIM
Lamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin.
B-type lamins undergo a series of modifications, such as farnesylation and phosphorylation. Increased phosphorylation of the lamins occurs before envelope disintegration and probably plays a role in regulating lamin associations.
Nucleus inner membrane>Lipid-anchor>Nucleoplasmic side.
Homodimer. Interacts with lamin-associated polypeptides IA, IB and 2. Interacts with SPAG4 and SEPT12.
Belongs to the intermediate filament family.
· Cellular Processes > Cell growth and death > Apoptosis.(View pathway)
AF5161-BP
(Blocking peptide available as AF5161-BP)
¥2000/1mg.
Tips: For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).
Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (immunoblot) and immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or immunostaining performed with the neutralized antibody.
Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
This product is for research use only. Not for use in diagnostic or therapeutic procedures.
Site | PTM Type | Enzyme | Source |
---|---|---|---|
A2 | Acetylation | Uniprot | |
T3 | Phosphorylation | Uniprot | |
T5 | Phosphorylation | Uniprot | |
R10 | Methylation | Uniprot | |
S13 | Phosphorylation | Uniprot | |
R14 | Methylation | Uniprot | |
T19 | Phosphorylation | Uniprot | |
T20 | Phosphorylation | Uniprot | |
S23 | Phosphorylation | P06493 (CDK1) | Uniprot |
T25 | Phosphorylation | Uniprot | |
S28 | Phosphorylation | Uniprot | |
K33 | Acetylation | Uniprot | |
K33 | Ubiquitination | Uniprot | |
R42 | Methylation | Uniprot | |
Y46 | Phosphorylation | Uniprot | |
K49 | Acetylation | Uniprot | |
K49 | Ubiquitination | Uniprot | |
S58 | Phosphorylation | Uniprot | |
K79 | Acetylation | Uniprot | |
K79 | Ubiquitination | Uniprot | |
Y82 | Phosphorylation | Uniprot | |
T84 | Phosphorylation | Uniprot | |
K102 | Acetylation | Uniprot | |
K102 | Sumoylation | Uniprot | |
K102 | Ubiquitination | Uniprot | |
K109 | Ubiquitination | Uniprot | |
K111 | Acetylation | Uniprot | |
K111 | Ubiquitination | Uniprot | |
K123 | Acetylation | Uniprot | |
K123 | Ubiquitination | Uniprot | |
K124 | Ubiquitination | Uniprot | |
S126 | Phosphorylation | Uniprot | |
K134 | Acetylation | Uniprot | |
K134 | Ubiquitination | Uniprot | |
Y138 | Phosphorylation | Uniprot | |
S144 | Phosphorylation | Uniprot | |
K145 | Ubiquitination | Uniprot | |
K156 | Acetylation | Uniprot | |
K156 | Ubiquitination | Uniprot | |
K157 | Acetylation | Uniprot | |
K157 | Ubiquitination | Uniprot | |
S158 | Phosphorylation | Uniprot | |
K167 | Ubiquitination | Uniprot | |
K181 | Acetylation | Uniprot | |
K181 | Ubiquitination | Uniprot | |
K182 | Ubiquitination | Uniprot | |
R197 | Methylation | Uniprot | |
C198 | S-Nitrosylation | Uniprot | |
S200 | Phosphorylation | Uniprot | |
K209 | Acetylation | Uniprot | |
K209 | Ubiquitination | Uniprot | |
S210 | Phosphorylation | Uniprot | |
Y212 | Phosphorylation | Uniprot | |
K222 | Ubiquitination | Uniprot | |
S232 | Phosphorylation | Uniprot | |
Y238 | Phosphorylation | Uniprot | |
K241 | Sumoylation | Uniprot | |
K241 | Ubiquitination | Uniprot | |
R250 | Methylation | Uniprot | |
K261 | Sumoylation | Uniprot | |
K261 | Ubiquitination | Uniprot | |
T267 | Phosphorylation | Uniprot | |
Y268 | Phosphorylation | Uniprot | |
K271 | Acetylation | Uniprot | |
K271 | Ubiquitination | Uniprot | |
S278 | Phosphorylation | Uniprot | |
S279 | Phosphorylation | Uniprot | |
T283 | Phosphorylation | Uniprot | |
S284 | Phosphorylation | Uniprot | |
T285 | Phosphorylation | Uniprot | |
S288 | Phosphorylation | Uniprot | |
R297 | Methylation | Uniprot | |
R299 | Methylation | Uniprot | |
S302 | Phosphorylation | Uniprot | |
S304 | Phosphorylation | Uniprot | |
S305 | Phosphorylation | Uniprot | |
K312 | Acetylation | Uniprot | |
K312 | Ubiquitination | Uniprot | |
K330 | Ubiquitination | Uniprot | |
T340 | Phosphorylation | Uniprot | |
K342 | Ubiquitination | Uniprot | |
Y360 | Phosphorylation | Uniprot | |
S375 | Phosphorylation | Uniprot | |
Y377 | Phosphorylation | Uniprot | |
K379 | Acetylation | Uniprot | |
K379 | Sumoylation | Uniprot | |
K379 | Ubiquitination | Uniprot | |
R387 | Methylation | Uniprot | |
K389 | Sumoylation | Uniprot | |
K389 | Ubiquitination | Uniprot | |
S391 | Phosphorylation | Uniprot | |
S393 | Phosphorylation | P06493 (CDK1) | Uniprot |
S395 | Phosphorylation | P05771 (PRKCB) | Uniprot |
S396 | Phosphorylation | Uniprot | |
T399 | Phosphorylation | Uniprot | |
S401 | Phosphorylation | Uniprot | |
S404 | Phosphorylation | Uniprot | |
S405 | Phosphorylation | P05771 (PRKCB) | Uniprot |
S406 | Phosphorylation | Uniprot | |
S408 | Phosphorylation | Uniprot | |
T411 | Phosphorylation | Uniprot | |
T412 | Phosphorylation | Uniprot | |
K457 | Ubiquitination | Uniprot | |
S460 | Phosphorylation | Uniprot | |
K474 | Ubiquitination | Uniprot | |
S479 | Phosphorylation | Uniprot | |
S481 | Phosphorylation | Uniprot | |
K483 | Acetylation | Uniprot | |
K483 | Ubiquitination | Uniprot | |
K528 | Ubiquitination | Uniprot | |
K532 | Acetylation | Uniprot | |
K532 | Ubiquitination | Uniprot | |
S534 | Phosphorylation | Uniprot | |
K547 | Ubiquitination | Uniprot | |
T575 | Phosphorylation | Uniprot | |
S579 | Phosphorylation | Uniprot |