ATG7 Antibody - #DF6130

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产品: ATG7 抗体
货号: DF6130
描述: Rabbit polyclonal antibody to ATG7
应用: WB IHC
反应: Human, Mouse, Rat
预测: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
分子量: 78kDa; 78kD(Calculated).
蛋白号: O95352
RRID: AB_2838097

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MS Report
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实验方案
WB Handbook
IHC Protocol
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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(90%), Bovine(100%), Horse(90%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(80%)
克隆:
Polyclonal
特异性:
ATG7 Antibody detects endogenous levels of total ATG7.
RRID:
AB_2838097
引用格式: Affinity Biosciences Cat# DF6130, RRID:AB_2838097.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

1810013K23Rik; Apg 7; APG7 autophagy 7 like; APG7 autophagy 7-like (S. cerevisiae); APG7 like; APG7, S. cerevisiae, homolog of; APG7-like; APG7L; ATG 7; ATG12-activating enzyme E1 ATG7; ATG7; ATG7 autophagy related 7 homolog (S. cerevisiae); ATG7 autophagy related 7 homolog; ATG7_HUMAN; Atg7l; Autophagy 7, S. cerevisiae, homolog of; Autophagy related protein 7; Autophagy-related 7 (yeast); Autophagy-related protein 7; DKFZp434N0735; GSA 7; GSA7; hAGP7; Ubiquitin activating enzyme E1 like protein; Ubiquitin-activating enzyme E1-like protein; Ubiquitin-like modifier-activating enzyme ATG7;

抗原和靶标

免疫原:
Uniprot:

O95352(ATG7_HUMAN) >>Visit HPA database.

基因/基因ID:
ATG7(10533)
表达:
O95352 ATG7_HUMAN:

Widely expressed, especially in kidney, liver, lymph nodes and bone marrow.

描述:
Autophagy is a catabolic process for the autophagosomic-lysosomal degradation of bulk cytoplasmic contents (1,2). Autophagy is generally activated by conditions of nutrient deprivation but has also been associated with a number of physiological processes including development, differentiation, neurodegeneration, infection, and cancer (3). The molecular machinery of autophagy was largely discovered in yeast and referred to as autophagy-related (Atg) genes. Formation of the autophagosome involves a ubiquitin-like conjugation system in which Atg12 is covalently bound to Atg5 and targeted to autophagosome vesicles (4-6). This conjugation reaction is mediated by the ubiquitin E1-like enzyme Atg7 and the E2-like enzyme Atg10 (7,8).
序列:
MAAATGDPGLSKLQFAPFSSALDVGFWHELTQKKLNEYRLDEAPKDIKGYYYNGDSAGLPARLTLEFSAFDMSAPTPARCCPAIGTLYNTNTLESFKTADKKLLLEQAANEIWESIKSGTALENPVLLNKFLLLTFADLKKYHFYYWFCYPALCLPESLPLIQGPVGLDQRFSLKQIEALECAYDNLCQTEGVTALPYFLIKYDENMVLVSLLKHYSDFFQGQRTKITIGVYDPCNLAQYPGWPLRNFLVLAAHRWSSSFQSVEVVCFRDRTMQGARDVAHSIIFEVKLPEMAFSPDCPKAVGWEKNQKGGMGPRMVNLSECMDPKRLAESSVDLNLKLMCWRLVPTLDLDKVVSVKCLLLGAGTLGCNVARTLMGWGVRHITFVDNAKISYSNPVRQPLYEFEDCLGGGKPKALAAADRLQKIFPGVNARGFNMSIPMPGHPVNFSSVTLEQARRDVEQLEQLIESHDVVFLLMDTRESRWLPAVIAASKRKLVINAALGFDTFVVMRHGLKKPKQQGAGDLCPNHPVASADLLGSSLFANIPGYKLGCYFCNDVVAPGDSTRDRTLDQQCTVSRPGLAVIAGALAVELMVSVLQHPEGGYAIASSSDDRMNEPPTSLGLVPHQIRGFLSRFDNVLPVSLAFDKCTACSSKVLDQYEREGFNFLAKVFNSSHSFLEDLTGLTLLHQETQAAEIWDMSDDETI

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Pig
90
Horse
90
Chicken
80
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - O95352 作为底物

Site PTM Type Enzyme
A2 Acetylation
T5 Phosphorylation
S11 Phosphorylation
Y38 Phosphorylation
T120 Phosphorylation
K300 Ubiquitination
K306 Acetylation
K306 Ubiquitination
K352 Ubiquitination
K411 Ubiquitination
R420 Methylation
S490 Phosphorylation
S531 Phosphorylation
K652 Ubiquitination
S698 Phosphorylation

研究背景

功能:

E1-like activating enzyme involved in the 2 ubiquitin-like systems required for cytoplasm to vacuole transport (Cvt) and autophagy. Activates ATG12 for its conjugation with ATG5 as well as the ATG8 family proteins for their conjugation with phosphatidylethanolamine. Both systems are needed for the ATG8 association to Cvt vesicles and autophagosomes membranes. Required for autophagic death induced by caspase-8 inhibition. Required for mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production. Modulates p53/TP53 activity to regulate cell cycle and survival during metabolic stress. Plays also a key role in the maintenance of axonal homeostasis, the prevention of axonal degeneration, the maintenance of hematopoietic stem cells, the formation of Paneth cell granules, as well as in adipose differentiation. Plays a role in regulating the liver clock and glucose metabolism by mediating the autophagic degradation of CRY1 (clock repressor) in a time-dependent manner (By similarity).

翻译修饰:

Acetylated by EP300.

细胞定位:

Cytoplasm. Preautophagosomal structure.
Note: Localizes also to discrete punctae along the ciliary axoneme and to the base of the ciliary axoneme.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Widely expressed, especially in kidney, liver, lymph nodes and bone marrow.

亚基结构:

Homodimer. Interacts with ATG3 and ATG12. The complex, composed of ATG3 and ATG7, plays a role in the conjugation of ATG12 to ATG5. Forms intermediate conjugates with ATG8-like proteins such as GABARAP, GABARAPL1, GABARAPL2 or MAP1LC3A. Interacts with EP300 acetyltransferase. Interacts with FOXO1.

蛋白家族:

The C-terminal part of the protein is essential for the dimerization and interaction with ATG3 and ATG12.

The N-terminal FAP motif (residues 15 to 17) is essential for the formation of the ATG89-PE and ATG5-ATG12 conjugates.

Belongs to the ATG7 family.

研究领域

· Cellular Processes > Transport and catabolism > Autophagy - other.   (View pathway)

· Cellular Processes > Transport and catabolism > Autophagy - animal.   (View pathway)

· Cellular Processes > Cell growth and death > Ferroptosis.   (View pathway)

文献引用

1). Integrin 51, as a Receptor of Fibronectin, Binds the FbaA Protein of Group A Streptococcus To Initiate Autophagy during Infection. mBio (PubMed: 32518187) [IF=6.4]
2). Lupeol inhibits the proliferation and migration of MDA-MB-231 breast cancer cells via a novel crosstalk mechanism between autophagy and the EMT. Food & Function (PubMed: 35448900) [IF=6.1]
3). Alginate oligosaccharides improve high-fat induced hepatic steatosis via PGC-1α-mediated lipophagy and fatty acid β-oxidation pathway. Journal of Functional Foods [IF=5.6]

Application: WB    Species: Mouse    Sample: livers

Fig. 4. Effects of AOS on enhancing the lipophagy and free fatty acid (FFA) β-oxidation level in the NAFLD mouse livers. (A) the mRNA expression in the lipophagy and FFA β-oxidation pathways, (B) western blot results of lipophagy pathway, (C) the protein levels of lipophagy pathway, (D) western blot results of FFA β-oxidation pathway, (E) the protein levels of FFA β-oxidation pathway. The data are shown as the mean ± SEM. Values with different letters represent significant differences (p < 0.05)..
4). Qingyihuaji Formula promotes apoptosis and autophagy through inhibition of MAPK/ERK and PI3K/Akt/mTOR signaling pathway on pancreatic cancer in vivo and in vitro. Journal of Ethnopharmacology (PubMed: 36690307) [IF=5.4]
5). Respiratory Syncytial Virus Replication Is Promoted by Autophagy-Mediated Inhibition of Apoptosis. Journal of Virology (PubMed: 29386287) [IF=5.4]

Application: WB    Species: human    Sample: Hep2 cells

Figure 5. |Inhibition of autophagy with specific shRNA targeting ATG5, ATG7 or BECN1 reduces RSV replication. (A-C) Hep2 cells stably expressing specific shRNA against ATG5 (Hep2-sh-ATG5), ATG7 (Hep2-sh-ATG7), BECN1 (Hep2-sh-BECN1) or luciferase (Hep2-sh-luciferase) were established as described in Materials and Methods. The silencing efficiency of ATG5 shRNA (A), ATG7 shRNA (B) and BECN1 shRNA (C) were validated by immunoblotting with anti-ATG5, anti-ATG7, anti-BECN1 and anti-GAPDH antibodies respectively.
6). Calycosin inhibited autophagy and oxidative stress in chronic kidney disease skeletal muscle atrophy by regulating AMPK/SKP2/CARM1 signalling pathway. JOURNAL OF CELLULAR AND MOLECULAR MEDICINE (PubMed: 32910538) [IF=5.3]

Application: IHC    Species: rat    Sample: muscles

FIGURE 4|Effect of calycosin on autophagy in muscle of 5/6 Nx rats. A, Representative images of autophagosomes observed in muscle of rat using TEM. B-E, Representative images and scores of LC3A/B and ATG7 expression in muscle of rats using IHC staining (×200). Bar = 50 µm. *P < 0.05 compared to the sham group. ##P < 0.01 compared to the 5/6 Nx model group

Application: WB    Species: rat    Sample: muscles

FIGURE 5|Effect of calycosin on the expression of CARM1 and H3R17me2a,and related proteins in rat muscles. A-B,Representative images and scores of the expression of CARM1 and H3R17me2a in muscle sections using IHC staining (×200).Bar = 50 µm. *P < 0.05 compared to the sham group. ##P < 0.01 compared to the 5/6 Nx model group. C-E, Representative immune blots of key proteins involved in muscle atrophy and proteins associated with the AMPK/SKP2/CARM1 signalling pathway
7). Pelargonic acid vanillylamide alleviates hepatic autophagy and ER stress in hepatic steatosis model. Food and Chemical Toxicology (PubMed: 37611858) [IF=4.3]
8). HIF-1α enhances autophagy to alleviate apoptosis in marginal cells in the stria vascular in neonatal rats under hypoxia. The International Journal of Biochemistry & Cell Biology (PubMed: 35779841) [IF=4.0]
9). Sporothrix globosa melanin regulates autophagy via the TLR2 signaling pathway in THP-1 macrophages. PLOS Neglected Tropical Diseases (PubMed: 37141335) [IF=3.8]

Application: WB    Species: Human    Sample:

S1 Fig. Western blot analysis confirming Atg7 knockdown by siRNA. (A) Western blotting of Atg7 was conducted in THP-1 macrophages with or without knockdown treatment. (B) The ratio of Atg7/GAPDH was calculated. *P < 0.05, for the unpaired t-test. Representative images are displayed.
10). Arbutin ameliorates glucocorticoid-induced osteoporosis through activating autophagy in osteoblasts. EXPERIMENTAL BIOLOGY AND MEDICINE (PubMed: 33757338) [IF=3.2]

Application: WB    Species: mice    Sample: MC3T3-E1 cells

Figure 3. Arbutin mitigated the inhibitory effects of Dex on autophagy in femur of osteoporotic mice. (a) Western blot analysis was performed to determine the levels of autophagy-related proteins. (b) Quantitative analysis of relative expression. Data represented as mean  SD (n ¼ 6/group). *P<0.05, **P<0. 01, ***P<0.001, ****P<0.0001. (A color version of this figure is available in the online journal.) GAPDH: glyceraldehyde 3-phosphate dehydrogenase.
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