产品: CD86 抗体
货号: DF6332
描述: Rabbit polyclonal antibody to CD86
应用: WB IHC IF/ICC
反应: Human, Mouse
分子量: 38kDa,60-85kD; 38kD(Calculated).
蛋白号: P42081
RRID: AB_2838296

浏览相似产品>>

   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

联系销售

产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse
克隆:
Polyclonal
特异性:
CD86 Antibody detects endogenous levels of total CD86.
RRID:
AB_2838296
引用格式: Affinity Biosciences Cat# DF6332, RRID:AB_2838296.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Activation B7-2 antigen 3; Activation B7-2 antigen; B-lymphocyte activation antigen B7-2 2; B-lymphocyte activation antigen B7-2; B7 2; B7; B7-2; B7.2; B70; B72 antigen; BU63; CD28 antigen ligand 2 2; CD28 antigen ligand 2; Cd28l2; CD28LG2; CD86; CD86 antigen (CD28 antigen ligand 2, B7-2 antigen) 1, 2; CD86 antigen (CD28 antigen ligand 2, B7-2 antigen); CD86 antigen; CD86 molecule; CD86_HUMAN; CLS1; CTLA-4 counter-receptor B7.2 2, 3; CTLA-4 counter-receptor B7.2; Early T-cell costimulatory molecule 1; ETC-1; FUN 1; FUN-1; LAB72; Ly-58; Ly58; MB7; MB7-2; Membrane glycoprotein; MGC34413; T-lymphocyte activation antigen CD86; TS/A-2;

抗原和靶标

免疫原:
Uniprot:
基因/基因ID:
表达:
P42081 CD86_HUMAN:

Expressed by activated B-lymphocytes and monocytes.

描述:
This gene encodes a type I membrane protein that is a member of the immunoglobulin superfamily. This protein is expressed by antigen-presenting cells, and it is the ligand for two proteins at the cell surface of T cells, CD28 antigen and cytotoxic T-lymphocyte-associated protein 4. Binding of this protein with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of this protein with cytotoxic T-lymphocyte-associated protein 4 negatively regulates T-cell activation and diminishes the immune response. Alternative splicing results in several transcript variants encoding different isoforms.[provided by RefSeq, May 2011]
序列:
MDPQCTMGLSNILFVMAFLLSGAAPLKIQAYFNETADLPCQFANSQNQSLSELVVFWQDQENLVLNEVYLGKEKFDSVHSKYMGRTSFDSDSWTLRLHNLQIKDKGLYQCIIHHKKPTGMIRIHQMNSELSVLANFSQPEIVPISNITENVYINLTCSSIHGYPEPKKMSVLLRTKNSTIEYDGVMQKSQDNVTELYDVSISLSVSFPDVTSNMTIFCILETDKTRLLSSPFSIELEDPQPPPDHIPWITAVLPTVIICVMVFCLILWKWKKKKRPRNSYKCGTNTMEREESEQTKKREKIHIPERSDEAQRVFKSSKTSSCDKSDTCF

翻译修饰 - P42081 作为底物

Site PTM Type Enzyme
S77 Phosphorylation
S80 Phosphorylation
Y108 Phosphorylation
T175 Phosphorylation
K315 Acetylation

研究背景

功能:

Receptor involved in the costimulatory signal essential for T-lymphocyte proliferation and interleukin-2 production, by binding CD28 or CTLA-4. May play a critical role in the early events of T-cell activation and costimulation of naive T-cells, such as deciding between immunity and anergy that is made by T-cells within 24 hours after activation. Isoform 2 interferes with the formation of CD86 clusters, and thus acts as a negative regulator of T-cell activation.

(Microbial infection) Acts as a receptor for adenovirus subgroup B.

翻译修饰:

Polyubiquitinated; which is promoted by MARCH8 and results in endocytosis and lysosomal degradation.

细胞定位:

Cell membrane>Single-pass type I membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Expressed by activated B-lymphocytes and monocytes.

亚基结构:

Homodimer. Interacts with MARCH8.

(Microbial infection) Interacts with adenovirus subgroup b fiber protein.

研究领域

· Environmental Information Processing > Signaling molecules and interaction > Cell adhesion molecules (CAMs).   (View pathway)

· Human Diseases > Endocrine and metabolic diseases > Type I diabetes mellitus.

· Human Diseases > Cancers: Overview > Transcriptional misregulation in cancer.

· Human Diseases > Immune diseases > Autoimmune thyroid disease.

· Human Diseases > Immune diseases > Systemic lupus erythematosus.

· Human Diseases > Immune diseases > Rheumatoid arthritis.

· Human Diseases > Immune diseases > Allograft rejection.

· Human Diseases > Immune diseases > Graft-versus-host disease.

· Human Diseases > Cardiovascular diseases > Viral myocarditis.

· Organismal Systems > Immune system > Toll-like receptor signaling pathway.   (View pathway)

· Organismal Systems > Immune system > Intestinal immune network for IgA production.   (View pathway)

文献引用

1). Toxin-Enabled “On-Demand” Liposomes for Enhanced Phototherapy to Treat and Protect against Methicillin-Resistant Staphylococcus aureus Infection. Small (PubMed: 35859534) [IF=13.3]

2). Bacterial Toxin‐Responsive Biomimetic Nanobubbles for Precision Photodynamic Therapy against Bacterial Infections. Advanced Healthcare Materials (PubMed: 35836329) [IF=10.0]

3). Multimodal imaging and photothermal synergistic immunotherapy of retinoblastoma with tuftsin-loaded carbonized MOF nanoparticles. DRUG DELIVERY (PubMed: 35642917) [IF=6.0]

Application: WB    Species: Mice    Sample: Y79 tumors

Figure 11. (A, B) The contents of CD86 in Y79 tumors after different treatments. (C) TNF-α levels in Y79 tumors after different treatments (**p<.01).

4). Isosteviol reduces the acute inflammatory response after burns by upregulating MMP9 in macrophages leading to M2 polarization. International Immunopharmacology (PubMed: 35176589) [IF=5.6]

5). Zanubrutinib ameliorates lipopolysaccharide-induced acute lung injury via regulating macrophage polarization. International Immunopharmacology (PubMed: 35973369) [IF=5.6]

6). miR-146a-5p enhances embryo survival in unexplained recurrent spontaneous abortion by promoting M2 polarization of decidual macrophages. International Immunopharmacology (PubMed: 35764020) [IF=5.6]

7). Vascular endothelial growth factor A is a potential prognostic biomarker and correlates with immune cell infiltration in hepatocellular carcinoma. Journal of Cellular and Molecular Medicine (PubMed: 36729917) [IF=5.3]

8). Contribution of Oxidative Stress Induced by Sonodynamic Therapy to the Calcium Homeostasis Imbalance Enhances Macrophage Infiltration in Glioma Cells. Cancers (PubMed: 35454942) [IF=5.2]

Application: IF/ICC    Species: Mouse    Sample: brain

Figure 8. (A) GL261 glioma-bearing C57BL/6 to undergo xenograft tumor and brain treatment and MRI tests on days 0 and 24. (B,D) At the end of the experiment (30 days), the mouse brains were made into sections for immunofluorescence staining (CD86 and CD206); (C,E) analysis of the immunofluorescence intensity showed that the SG group induced more CD86 expression in the gliomas than CD206 expression.

9). Erythropoietin-Induced Autophagy Protects Against Spinal Cord Injury and Improves Neurological Function via the Extracellular-Regulated Protein Kinase Signaling Pathway. MOLECULAR NEUROBIOLOGY (PubMed: 32647973) [IF=5.1]

Application: IF/ICC    Species: rat    Sample:

Fig. 6 |EPO benefit antiinflammation and axonal regeneration can be neutralized by ERK inhibition, and activator can mimic the effect of EPO. a The axonal special protein GAP43 immunofluorescence staining and histogram at 14 days (magnification × 400). b–e The inflammatory marker proteins immunofluorescence staining and histograms at 14 days, including CD86, GFAP, TNF-α, iNOS(magnification × 400). All data presented as mean ± SEM in each group. *p < 0.05 vs. SCI + saline group, **p < 0.01 vs. SCI + saline group. #p < 0.05 comparison between SCI + EPO group and SCI + EPO + PD98059, ##p < 0.01 comparison between SCI + EPO group and SCI + EPO + PD98059

10). Protecting effect of emodin in experimental autoimmune encephalomyelitis mice by inhibiting microglia activation and inflammation via Myd88/PI3K/Akt/NF-κB signalling pathway. Bioengineered (PubMed: 35287559) [IF=4.9]

Application: IF/ICC    Species: Mice    Sample: brain tissue

Emodin contributed to inhibition of the microglia activation. Double immunofluorescence staining of brain tissue with M1(CD86+/iba1+) (a) and M2(CD206+/iba1+) (b). The representative double immunofluorescence labeling image of CD86 (red)/Iba1 (green) in brain tissue, and the nuclei were stained with DAPI (blue). The representative double immunofluorescence labeling image of CD206 (red)/Iba1 (green) in brain tissue and the nuclei were stained with DAPI (blue). The quantity of the percentage of CD86+/Iba1+ cell (c) and CD206+/Iba1+ cell. (d) The effects of emodin on M1 microglia cell subsets mRNA relative expression. Mice were sacrificed on 21 dpi. The CNS tissues are collected. The IL-6 (e), TGF-β (f), IL-17A (g), and RORγt mRNA relative expression were analyzed by qRT-PCR, respectively. All data were expressed as mean ± S.D (n = 5). Comparisons among each group were analyzed by one-way ANOVA in C, D, F, and G. Comparisons among each group were analyzed by Kruskal-Wallis test using Bonferroni comparisons post hoc tests in E and H. *P < 0.05, **P < 0.01 versus NC group, #P < 0.05, ##P < 0.01 versus EAE group.

加载更多

限制条款

产品的规格、报价、验证数据请以官网为准,官网链接:www.affbiotech.com | www.affbiotech.cn(简体中文)| www.affbiotech.jp(日本語)

产品的数据信息为Affinity所有,未经授权不得收集Affinity官网数据或资料用于商业用途,对抄袭产品数据的行为我们将保留诉诸法律的权利。

产品相关数据会因产品批次、产品检测情况随时调整,如您已订购该产品,请以订购时随货说明书为准,否则请以官网内容为准,官网内容有改动时恕不另行通知。

Affinity保证所销售产品均经过严格质量检测。如您购买的商品在规定时间内出现问题需要售后时,请您在Affinity官方渠道提交售后申请。

产品仅供科学研究使用。不用于诊断和治疗。 

产品未经授权不得转售。

Affinity Biosciences将不会对在使用我们的产品时可能发生的专利侵权或其他侵权行为负责。Affinity Biosciences, Affinity Biosciences标志和所有其他商标所有权归Affinity Biosciences LTD.