EPXH2 Antibody - #DF6676

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产品: EPXH2 抗体
货号: DF6676
描述: Rabbit polyclonal antibody to EPXH2
应用: WB IHC IF/ICC
反应: Human, Mouse, Rat, Monkey
预测: Pig, Bovine, Horse, Sheep, Rabbit
分子量: 62kDa; 63kD(Calculated).
蛋白号: P34913
RRID: AB_2838638

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat,Monkey
预测:
Pig(91%), Bovine(91%), Horse(91%), Sheep(91%), Rabbit(91%)
克隆:
Polyclonal
特异性:
EPXH2 Antibody detects endogenous levels of total EPXH2.
RRID:
AB_2838638
引用格式: Affinity Biosciences Cat# DF6676, RRID:AB_2838638.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Bifunctional epoxide hydrolase 2; CEH; Cytosolic epoxide hydrolase; EPHX2; Epoxide hydratase; Epoxide hydrolase 2; Epoxide hydrolase 2 cytoplasmic; epoxide hydrolase 2, cytosolic; Epoxide hydrolase soluble; HYES_HUMAN; SEH; Soluble epoxide hydrolase;

抗原和靶标

免疫原:
Uniprot:

P34913(HYES_HUMAN) >>Visit HPA database.

基因/基因ID:
EPHX2(2053)
描述:
This gene encodes a member of the epoxide hydrolase family. The protein, found in both the cytosol and peroxisomes,binds to specific epoxides and converts them to the corresponding dihydrodiols. Mutations in this gene have beenassociated with familial hypercholesterolemia. Alternatively spliced transcript variants have been described.
序列:
MTLRAAVFDLDGVLALPAVFGVLGRTEEALALPRGLLNDAFQKGGPEGATTRLMKGEITLSQWIPLMEENCRKCSETAKVCLPKNFSIKEIFDKAISARKINRPMLQAALMLRKKGFTTAILTNTWLDDRAERDGLAQLMCELKMHFDFLIESCQVGMVKPEPQIYKFLLDTLKASPSEVVFLDDIGANLKPARDLGMVTILVQDTDTALKELEKVTGIQLLNTPAPLPTSCNPSDMSHGYVTVKPRVRLHFVELGSGPAVCLCHGFPESWYSWRYQIPALAQAGYRVLAMDMKGYGESSAPPEIEEYCMEVLCKEMVTFLDKLGLSQAVFIGHDWGGMLVWYMALFYPERVRAVASLNTPFIPANPNMSPLESIKANPVFDYQLYFQEPGVAEAELEQNLSRTFKSLFRASDESVLSMHKVCEAGGLFVNSPEEPSLSRMVTEEEIQFYVQQFKKSGFRGPLNWYRNMERNWKWACKSLGRKILIPALMVTAEKDFVLVPQMSQHMEDWIPHLKRGHIEDCGHWTQMDKPTEVNQILIKWLDSDARNPPVVSKM

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
91
Horse
91
Bovine
91
Sheep
91
Rabbit
91
Zebrafish
73
Xenopus
70
Dog
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P34913 作为底物

Site PTM Type Enzyme
K43 Acetylation
K43 Ubiquitination
K55 Ubiquitination
K79 Ubiquitination
K84 Ubiquitination
K89 Ubiquitination
K94 Acetylation
K94 Ubiquitination
K114 Acetylation
K115 Acetylation
K167 Acetylation
K174 Acetylation
K174 Ubiquitination
K191 Ubiquitination
K245 Ubiquitination
Y296 Phosphorylation
S299 Phosphorylation
Y308 Phosphorylation
S370 Phosphorylation
K406 Ubiquitination
S412 Phosphorylation
K421 Ubiquitination
K455 Acetylation
K474 Ubiquitination
K478 Ubiquitination
K483 Ubiquitination
K515 Ubiquitination
K530 Ubiquitination
K540 Ubiquitination
K554 Ubiquitination

研究背景

功能:

Bifunctional enzyme. The C-terminal domain has epoxide hydrolase activity and acts on epoxides (alkene oxides, oxiranes) and arene oxides. Plays a role in xenobiotic metabolism by degrading potentially toxic epoxides (By similarity). Also determines steady-state levels of physiological mediators. The N-terminal domain has lipid phosphatase activity, with the highest activity towards threo-9,10-phosphonooxy-hydroxy-octadecanoic acid, followed by erythro-9,10-phosphonooxy-hydroxy-octadecanoic acid, 12-phosphonooxy-octadec-9Z-enoic acid and 12-phosphonooxy-octadec-9E-enoic acid.

翻译修饰:

The N-terminus is blocked.

The covalent modification of cysteine by 15-deoxy-Delta12,14-prostaglandin-J2 is autocatalytic and reversible. It may occur as an alternative to other cysteine modifications, such as S-nitrosylation and S-palmitoylation (Probable).

细胞定位:

Cytoplasm. Peroxisome.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
亚基结构:

Homodimer.

蛋白家族:

The N-terminal domain has phosphatase activity. The C-terminal domain has epoxide hydrolase activity.

Belongs to the AB hydrolase superfamily. Epoxide hydrolase family.

研究领域

· Cellular Processes > Transport and catabolism > Peroxisome.   (View pathway)

· Metabolism > Lipid metabolism > Arachidonic acid metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

文献引用

1). Ginsenoside compound K alleviates sodium valproate-induced hepatotoxicity in rats via antioxidant effect, regulation of peroxisome pathway and iron homeostasis. TOXICOLOGY AND APPLIED PHARMACOLOGY (PubMed: 31734319) [IF=3.8]

Application: WB    Species: rat    Sample: liver

Fig. 7| Effect of SVP alone and combined with G-CK or L-Car on hepatic soluble epoxide hydrolase protein level. A: Western blot analysis
2). Evaluating the protective effects of individual or combined ginsenoside compound K and the downregulation of soluble epoxide hydrolase expression against sodium valproate-induced liver cell damage. Toxicology and Applied Pharmacology (PubMed: 33915122) [IF=3.8]

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