GCLM Antibody - #DF7268

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产品: GCLM 抗体
货号: DF7268
描述: Rabbit polyclonal antibody to GCLM
应用: WB IHC
反应: Human, Mouse, Rat, Pig
预测: Pig, Bovine, Rabbit
分子量: 30kDa; 31kD(Calculated).
蛋白号: P48507
RRID: AB_2839207

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 50ul RMB¥ 1250 现货
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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat,Pig
预测:
Bovine(100%), Rabbit(100%)
克隆:
Polyclonal
特异性:
GCLM Antibody detects endogenous levels of total GCLM.
RRID:
AB_2839207
引用格式: Affinity Biosciences Cat# DF7268, RRID:AB_2839207.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Gamma ECS regulatory subunit; Gamma-ECS regulatory subunit; Gamma-glutamylcysteine synthetase regulatory subunit; GCLM; GCS light chain; GLCLR; Glutamate cysteine ligase regulatory subunit; Glutamate--cysteine ligase modifier subunit; Glutamate--cysteine ligase regulatory subunit; GSC light chain; GSH0_HUMAN;

抗原和靶标

免疫原:
Uniprot:

P48507(GSH0_HUMAN) >>Visit HPA database.

基因/基因ID:
GCLM(2730)
表达:
P48507 GSH0_HUMAN:

In all tissues examined. Highest levels in skeletal muscle.

描述:
Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase, is the first rate limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. Gamma glutamylcysteine synthetase deficiency has been implicated in some forms of hemolytic anemia.
序列:
MGTDSRAAKALLARARTLHLQTGNLLNWGRLRKKCPSTHSEELHDCIQKTLNEWSSQINPDLVREFPDVLECTVSHAVEKINPDEREEMKVSAKLFIVESNSSSSTRSAVDMACSVLGVAQLDSVIIASPPIEDGVNLSLEHLQPYWEELENLVQSKKIVAIGTSDLDKTQLEQLYQWAQVKPNSNQVNLASCCVMPPDLTAFAKQFDIQLLTHNDPKELLSEASFQEALQESIPDIQAHEWVPLWLLRYSVIVKSRGIIKSKGYILQAKRRGS

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
100
Bovine
100
Rabbit
100
Xenopus
78
Horse
0
Sheep
0
Dog
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P48507 作为底物

Site PTM Type Enzyme
K9 Sumoylation
K9 Ubiquitination
T17 Phosphorylation
K34 Ubiquitination
C35 S-Nitrosylation
K49 Ubiquitination
C72 S-Nitrosylation
K80 Ubiquitination
K90 Ubiquitination
K158 Sumoylation
K158 Ubiquitination
T164 Phosphorylation
S165 Phosphorylation
K169 Ubiquitination
K205 Sumoylation
K263 Acetylation
K263 Ubiquitination
Y265 Phosphorylation
K270 Ubiquitination

研究背景

组织特异性:

In all tissues examined. Highest levels in skeletal muscle.

亚基结构:

Heterodimer of a catalytic heavy chain and a regulatory light chain.

蛋白家族:

Belongs to the aldo/keto reductase family. Glutamate--cysteine ligase light chain subfamily.

研究领域

· Cellular Processes > Cell growth and death > Ferroptosis.   (View pathway)

· Metabolism > Amino acid metabolism > Cysteine and methionine metabolism.

· Metabolism > Metabolism of other amino acids > Glutathione metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

文献引用

1). Gly-Pro-Ala peptide and FGSHF3 exert protective effects in DON-induced toxicity and intestinal damage via decreasing oxidative stress. FOOD RESEARCH INTERNATIONAL (PubMed: 33509464) [IF=8.1]
2). Total flavonoids of Inula japonica alleviated the inflammatory response and oxidative stress in LPS-induced acute lung injury via inhibiting the sEH activity: Insights from lipid metabolomics. PHYTOMEDICINE (PubMed: 36150346) [IF=7.9]
3). Itaconate inhibits ferroptosis of macrophage via Nrf2 pathways against sepsis-induced acute lung injury. Cell Death Discovery (PubMed: 35110526) [IF=7.0]

Application: WB    Species: Mice    Sample: lung tissues

Fig. 3 4-OI inhibits ferroptosis through increasing and activating Nrf2 in vivo during ALI. A Western blots for Nrf2, GCLM, HO-1 and SLC7A11 in murine lung tissue. B–E Relative mRNA levels of Nrf2, GCLM, HO-1 and SLC7A11 in murine lung tissue (n = 6). F, G Relative levels of GSH and GSH/GSSG ratio in murine lung tissue (n = 6). H, I Representative images of fluorescence probe for ROS and its statistical results (n = 6) in lung tissue. (Data are presented as Mean ± SD *p < 0.05, **p < 0.01, ***p < 0.001).
4). Therapeutic role of D-pinitol on experimental colitis via activating Nrf2/ARE and PPAR-γ/NF-κB signaling pathways. Food & Function (PubMed: 33625409) [IF=6.1]
5). Ameliorate effect of pyrroloquinoline quinone against cyclophosphamide-induced nephrotoxicity by activating the Nrf2 pathway and inhibiting the NLRP3 pathway. LIFE SCIENCES (PubMed: 32504759) [IF=6.1]

Application: WB    Species: Mice    Sample: kidney tissues

Fig. 6. Effect of PQQ on protein expression in the Nrf2/HO-1 pathway. The expression levels of Nrf2 (A), HO-1 (B), GCLM (C), and NQO1 (D). Data are shown as mean ± SD, n = 8. #p < 0.05 and ##p < 0.01 versus the Control group. ⁎ p < 0.05 and ⁎⁎p < 0.01 versus the Model group.
6). Pyrroloquinoline quinone ameliorates liver injury in mice induced by cyclophosphamide. Environmental Science and Pollution Research (PubMed: 34997497) [IF=5.8]
7). Hepatoprotective and Anti-Oxidative Effects of Total Flavonoids From Qu Zhi Qiao (Fruit of Citrus Paradisi cv.Changshanhuyou) on Nonalcoholic Steatohepatitis In Vivo and In Vitro Through Nrf2-ARE Signaling Pathway. Frontiers in Pharmacology (PubMed: 32390839) [IF=5.6]

Application: WB    Species: mouse    Sample: liver

FIGURE 5 | Expression of the targeted proteins in mice liver. Values are shown as mean ± SD (n = 8). Data (mean ± SD) with different case letters are statistically different with each other at LSD multiple comparisons, which with red letters differed significantly as compared to model group level. uppercase letter p < 0.01 vs.another group.
8). Novel amphibian-derived antioxidant peptide protects skin against ultraviolet irradiation damage. Journal of photochemistry and photobiology-B, Biology (PubMed: 34628205) [IF=5.4]
9). Sodium Danshensu Cream Promotes the Healing of Pressure Ulcers in Mice through the Nrf2/HO-1 and NF-κB Pathways. Pharmaceuticals (PubMed: 36558999) [IF=4.6]

Application: WB    Species: Mouse    Sample:

Figure 6. Effect of SDSS on the protein levels of the Nrf2/HO-1 pathway (n = 5). (A) Western blot; the protein expression levels of Keap1 (B), Nrf2 (C), HO-1 (D), NQO1 (E), and GCLM (F) levels. * p < 0.05, ** p < 0.01 when compared to the control group. # p < 0.05, ## p < 0.01 when compared to the positive group.
10). Hepatoprotective effect of pyrroloquinoline quinone against alcoholic liver injury through activating Nrf2-mediated antioxidant and inhibiting TLR4-mediated inflammation responses. PROCESS BIOCHEMISTRY [IF=4.4]
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