ACTA1/A2/C1 Antibody - #DF2653

(1)  
产品: ACTA1/A2/C1 抗体
货号: DF2653
描述: Rabbit polyclonal antibody to ACTA1/A2/C1
应用: WB
反应: Human, Mouse, Rat
预测: Pig, Bovine, Sheep, Rabbit, Chicken, Xenopus
分子量: 42 kDa; 42kD(Calculated).
蛋白号: P68133 | P62736 | P68032
RRID: AB_2839859

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 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(100%), Bovine(100%), Sheep(100%), Rabbit(100%), Chicken(100%), Xenopus(86%)
克隆:
Polyclonal
特异性:
ACTA1/A2/C1 Antibody detects endogenous levels of total ACTA1/A2/C1.
RRID:
AB_2839859
引用格式: Affinity Biosciences Cat# DF2653, RRID:AB_2839859.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

a actin; ACTA; ACTA1; ACTA2; ACTC; ACTC1; Actin; ACTS_HUMAN; ACTSA; Alpha 2 actin; alpha skeletal muscle; Alpha-actin-1; Cardiac muscle alpha actin 1; Skeletal muscle alpha actin 1; a actin; AAT6; ACTA_HUMAN; ACTA2; Actin alpha 2 smooth muscle aorta; Actin aortic smooth muscle; Actin, aortic smooth muscle; ACTSA; ACTVS; Alpha 2 actin; Alpha actin 2; Alpha cardiac actin; Alpha-actin-2; Cell growth inhibiting gene 46 protein; Cell growth-inhibiting gene 46 protein; GIG46; Growth inhibiting gene 46; MYMY5; ACTC; ACTC_HUMAN; ACTC1; Actin alpha cardiac muscle 1; Actin; alpha cardiac muscle 1; Alpha-cardiac actin; ASD5; CMD1R; CMH11; LVNC4;

抗原和靶标

免疫原:
Uniprot:

P68133(ACTS_HUMAN) >>Visit HPA database.

P62736(ACTA_HUMAN) >>Visit HPA database.

P68032(ACTC_HUMAN) >>Visit HPA database.

基因/基因ID:
ACTA1(58) | ACTC1(70) | ACTA2(59)
描述:
Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.;
序列:
MCDEDETTALVCDNGSGLVKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPTLLTEAPLNPKANREKMTQIMFETFNVPAMYVAIQAVLSLYASGRTTGIVLDSGDGVTHNVPIYEGYALPHAIMRLDLAGRDLTDYLMKILTERGYSFVTTAEREIVRDIKEKLCYVALDFENEMATAASSSSLEKSYELPDGQVITIGNERFRCPETLFQPSFIGMESAGIHETTYNSIMKCDIDIRKDLYANNVMSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWITKQEYDEAGPSIVHRKCF

MCEEEDSTALVCDNGSGLCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIITNWDDMEKIWHHSFYNELRVAPEEHPTLLTEAPLNPKANREKMTQIMFETFNVPAMYVAIQAVLSLYASGRTTGIVLDSGDGVTHNVPIYEGYALPHAIMRLDLAGRDLTDYLMKILTERGYSFVTTAEREIVRDIKEKLCYVALDFENEMATAASSSSLEKSYELPDGQVITIGNERFRCPETLFQPSFIGMESAGIHETTYNSIMKCDIDIRKDLYANNVLSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKQEYDEAGPSIVHRKCF

MCDDEETTALVCDNGSGLVKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPTLLTEAPLNPKANREKMTQIMFETFNVPAMYVAIQAVLSLYASGRTTGIVLDSGDGVTHNVPIYEGYALPHAIMRLDLAGRDLTDYLMKILTERGYSFVTTAEREIVRDIKEKLCYVALDFENEMATAASSSSLEKSYELPDGQVITIGNERFRCPETLFQPSFIGMESAGIHETTYNSIMKCDIDIRKDLYANNVLSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKQEYDEAGPSIVHRKCF

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
100
Bovine
100
Sheep
100
Chicken
100
Rabbit
100
Xenopus
86
Horse
0
Dog
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P68133/P62736/P68032 作为底物

Site PTM Type Enzyme
E3 Acetylation
S35 Phosphorylation
K52 Acetylation
K52 Methylation
K52 Ubiquitination
S54 Phosphorylation
Y55 Phosphorylation
S62 Phosphorylation
K63 Acetylation
K63 Sumoylation
K63 Ubiquitination
T68 Phosphorylation
K70 Acetylation
K70 Methylation
K70 Ubiquitination
Y71 Phosphorylation
T79 Phosphorylation
K86 Acetylation
K86 Methylation
S91 Phosphorylation
Y93 Phosphorylation
R97 Methylation
K115 Acetylation
K115 Ubiquitination
T128 Phosphorylation
Y145 Phosphorylation
T151 Phosphorylation
S157 Phosphorylation
Y168 Phosphorylation
Y171 Phosphorylation
T188 Phosphorylation
Y190 Phosphorylation
K193 Acetylation
K193 Methylation
K193 Ubiquitination
T196 Phosphorylation
R198 Methylation
Y200 Phosphorylation
S201 Phosphorylation
T204 Phosphorylation
K215 Acetylation
K215 Ubiquitination
K217 Acetylation
K217 Methylation
C219 S-Nitrosylation
Y220 Phosphorylation
S235 Phosphorylation
S236 Phosphorylation
S237 Phosphorylation
K240 Ubiquitination
S241 Phosphorylation
Y242 Phosphorylation
T251 Phosphorylation
C259 S-Nitrosylation
T262 Phosphorylation
S267 Phosphorylation
T280 Phosphorylation
Y281 Phosphorylation
S283 Phosphorylation
C287 S-Nitrosylation
K293 Ubiquitination
Y296 Phosphorylation
T305 Phosphorylation
T306 Phosphorylation
Y308 Phosphorylation
K317 Acetylation
K317 Sumoylation
K317 Ubiquitination
T320 Phosphorylation
S325 Phosphorylation
T326 Phosphorylation
K328 Acetylation
K328 Methylation
K328 Sumoylation
K328 Ubiquitination
K330 Acetylation
K330 Sumoylation
K330 Ubiquitination
K338 Sumoylation
S350 Phosphorylation
Y364 Phosphorylation
S370 Phosphorylation
K375 Ubiquitination
C376 S-Nitrosylation
Site PTM Type Enzyme
S35 Phosphorylation
K52 Acetylation
K52 Methylation
K52 Ubiquitination
S54 Phosphorylation
Y55 Phosphorylation
S62 Phosphorylation
K63 Acetylation
K63 Sumoylation
K63 Ubiquitination
T68 Phosphorylation
K70 Acetylation
K70 Methylation
K70 Ubiquitination
Y71 Phosphorylation
T79 Phosphorylation
K86 Acetylation
K86 Methylation
K86 Sumoylation
K86 Ubiquitination
T91 Phosphorylation
Y93 Phosphorylation
K115 Acetylation
K115 Ubiquitination
T128 Phosphorylation
Y145 Phosphorylation
T151 Phosphorylation
S157 Phosphorylation
Y168 Phosphorylation
Y171 Phosphorylation
T188 Phosphorylation
Y190 Phosphorylation
K193 Acetylation
K193 Methylation
K193 Ubiquitination
T196 Phosphorylation
R198 Methylation
Y200 Phosphorylation
S201 O-Glycosylation
S201 Phosphorylation
T204 Phosphorylation
K215 Acetylation
K215 Ubiquitination
K217 Acetylation
K217 Methylation
Y220 Phosphorylation
S235 Phosphorylation
S236 Phosphorylation
S237 Phosphorylation
K240 Ubiquitination
S241 Phosphorylation
Y242 Phosphorylation
T251 Phosphorylation
T262 Phosphorylation
S267 Phosphorylation
T280 Phosphorylation
Y281 Phosphorylation
S283 Phosphorylation
Y296 Phosphorylation
T305 Phosphorylation
T306 Phosphorylation
Y308 Phosphorylation
K317 Acetylation
K317 Sumoylation
K317 Ubiquitination
T320 Phosphorylation
S325 Phosphorylation
T326 Phosphorylation
K328 Acetylation
K328 Methylation
K328 Sumoylation
K328 Ubiquitination
K330 Acetylation
K330 Sumoylation
K330 Ubiquitination
K338 Sumoylation
Y364 Phosphorylation
S370 Phosphorylation
K375 Ubiquitination
Site PTM Type Enzyme
S35 Phosphorylation
K52 Acetylation
K52 Methylation
K52 Ubiquitination
S54 Phosphorylation
Y55 Phosphorylation
S62 Phosphorylation
K63 Acetylation
K63 Sumoylation
K63 Ubiquitination
T68 Phosphorylation
K70 Acetylation
K70 Methylation
K70 Ubiquitination
Y71 Phosphorylation
T79 Phosphorylation
K86 Acetylation
K86 Methylation
K86 Sumoylation
K86 Ubiquitination
T91 Phosphorylation
Y93 Phosphorylation
K115 Acetylation
K115 Ubiquitination
T128 Phosphorylation
Y145 Phosphorylation
T151 Phosphorylation
S157 Phosphorylation
Y168 Phosphorylation
Y171 Phosphorylation
T188 Phosphorylation
Y190 Phosphorylation
K193 Acetylation
K193 Methylation
K193 Ubiquitination
T196 Phosphorylation
R198 Methylation
Y200 Phosphorylation
S201 Phosphorylation
T204 Phosphorylation
K215 Acetylation
K215 Ubiquitination
K217 Methylation
C219 S-Nitrosylation
Y220 Phosphorylation
S235 Phosphorylation
S236 Phosphorylation
S237 Phosphorylation
K240 Ubiquitination
S241 Phosphorylation
Y242 Phosphorylation
T251 Phosphorylation
T262 Phosphorylation
S267 Phosphorylation
T280 Phosphorylation
Y281 Phosphorylation
S283 Phosphorylation
K293 Ubiquitination
Y296 Phosphorylation
T306 Phosphorylation
Y308 Phosphorylation
K317 Sumoylation
K317 Ubiquitination
T320 Phosphorylation
S325 Phosphorylation
T326 Phosphorylation
K328 Acetylation
K328 Methylation
K328 Sumoylation
K328 Ubiquitination
K330 Acetylation
K330 Sumoylation
K330 Ubiquitination
K338 Sumoylation
S350 Phosphorylation
Y364 Phosphorylation
S370 Phosphorylation
K375 Ubiquitination

研究背景

功能:

Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.

翻译修饰:

Oxidation of Met-46 and Met-49 by MICALs (MICAL1, MICAL2 or MICAL3) to form methionine sulfoxide promotes actin filament depolymerization. MICAL1 and MICAL2 produce the (R)-S-oxide form. The (R)-S-oxide form is reverted by MSRB1 and MSRB2, which promotes actin repolymerization.

Monomethylation at Lys-86 (K84me1) regulates actin-myosin interaction and actomyosin-dependent processes. Demethylation by ALKBH4 is required for maintaining actomyosin dynamics supporting normal cleavage furrow ingression during cytokinesis and cell migration.

Methylated at His-75 by SETD3.

(Microbial infection) Monomeric actin is cross-linked by V.cholerae toxins RtxA and VgrG1 in case of infection: bacterial toxins mediate the cross-link between Lys-52 of one monomer and Glu-272 of another actin monomer, resulting in formation of highly toxic actin oligomers that cause cell rounding. The toxin can be highly efficient at very low concentrations by acting on formin homology family proteins: toxic actin oligomers bind with high affinity to formins and adversely affect both nucleation and elongation abilities of formins, causing their potent inhibition in both profilin-dependent and independent manners.

细胞定位:

Cytoplasm>Cytoskeleton.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
亚基结构:

Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others. Identified in a complex composed of ACTA1, COBL, GSN AND TMSB4X (By similarity). Interacts with TTID. Interacts (via its C-terminus) with USP25; the interaction occurs for all USP25 isoforms but is strongest for isoform USP25m in muscle differentiating cells.

蛋白家族:

Belongs to the actin family.

功能:

Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.

翻译修饰:

Oxidation of Met-46 and Met-49 by MICALs (MICAL1, MICAL2 or MICAL3) to form methionine sulfoxide promotes actin filament depolymerization. MICAL1 and MICAL2 produce the (R)-S-oxide form. The (R)-S-oxide form is reverted by MSRB1 and MSRB2, which promotes actin repolymerization.

Monomethylation at Lys-86 (K84me1) regulates actin-myosin interaction and actomyosin-dependent processes. Demethylation by ALKBH4 is required for maintaining actomyosin dynamics supporting normal cleavage furrow ingression during cytokinesis and cell migration.

Methylated at His-75 by SETD3.

(Microbial infection) Monomeric actin is cross-linked by V.cholerae toxins RtxA and VgrG1 in case of infection: bacterial toxins mediate the cross-link between Lys-52 of one monomer and Glu-272 of another actin monomer, resulting in formation of highly toxic actin oligomers that cause cell rounding. The toxin can be highly efficient at very low concentrations by acting on formin homology family proteins: toxic actin oligomers bind with high affinity to formins and adversely affect both nucleation and elongation abilities of formins, causing their potent inhibition in both profilin-dependent and independent manners.

细胞定位:

Cytoplasm>Cytoskeleton.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
亚基结构:

Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others.

蛋白家族:

Belongs to the actin family.

功能:

Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.

翻译修饰:

Oxidation of Met-46 and Met-49 by MICALs (MICAL1, MICAL2 or MICAL3) to form methionine sulfoxide promotes actin filament depolymerization. MICAL1 and MICAL2 produce the (R)-S-oxide form. The (R)-S-oxide form is reverted by MSRB1 and MSRB2, which promotes actin repolymerization.

Monomethylation at Lys-86 (K86me1) regulates actin-myosin interaction and actomyosin-dependent processes. Demethylation by ALKBH4 is required for maintaining actomyosin dynamics supporting normal cleavage furrow ingression during cytokinesis and cell migration.

Methylated at His-75 by SETD3.

(Microbial infection) Monomeric actin is cross-linked by V.cholerae toxins RtxA and VgrG1 in case of infection: bacterial toxins mediate the cross-link between Lys-52 of one monomer and Glu-272 of another actin monomer, resulting in formation of highly toxic actin oligomers that cause cell rounding. The toxin can be highly efficient at very low concentrations by acting on formin homology family proteins: toxic actin oligomers bind with high affinity to formins and adversely affect both nucleation and elongation abilities of formins, causing their potent inhibition in both profilin-dependent and independent manners.

细胞定位:

Cytoplasm>Cytoskeleton.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
亚基结构:

Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others.

蛋白家族:

Belongs to the actin family.

研究领域

· Environmental Information Processing > Signal transduction > Apelin signaling pathway.   (View pathway)

· Human Diseases > Cardiovascular diseases > Hypertrophic cardiomyopathy (HCM).

· Human Diseases > Cardiovascular diseases > Dilated cardiomyopathy (DCM).

· Organismal Systems > Circulatory system > Cardiac muscle contraction.   (View pathway)

· Organismal Systems > Circulatory system > Adrenergic signaling in cardiomyocytes.   (View pathway)

· Organismal Systems > Circulatory system > Vascular smooth muscle contraction.   (View pathway)

· Organismal Systems > Endocrine system > Relaxin signaling pathway.

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