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| 产品: | MMP12 抗体 |
| 货号: | DF7686 |
| 描述: | Rabbit polyclonal antibody to MMP12 |
| 应用: | WB IF/ICC |
| 反应: | Human, Monkey |
| 预测: | Rabbit |
| 分子量: | 54 kDa; 54kD(Calculated). |
| 蛋白号: | P39900 |
| RRID: | AB_2841157 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF7686, RRID:AB_2841157.
展开/折叠
EC 3.4.24.65; HME; Macrophage elastase; Macrophage metalloelastase; Macrophage metaloelastase; Matrix metallopeptidase 12 (macrophage elastase); Matrix metalloprotease 12; Matrix metalloproteinase-12; ME; MGC138506; MME; MMP 12; MMP-12; Mmp12; MMP12_HUMAN;
抗原和靶标
- P39900 MMP12_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MKFLLILLLQATASGALPLNSSTSLEKNNVLFGERYLEKFYGLEINKLPVTKMKYSGNLMKEKIQEMQHFLGLKVTGQLDTSTLEMMHAPRCGVPDVHHFREMPGGPVWRKHYITYRINNYTPDMNREDVDYAIRKAFQVWSNVTPLKFSKINTGMADILVVFARGAHGDFHAFDGKGGILAHAFGPGSGIGGDAHFDEDEFWTTHSGGTNLFLTAVHEIGHSLGLGHSSDPKAVMFPTYKYVDINTFRLSADDIRGIQSLYGDPKENQRLPNPDNSEPALCDPNLSFDAVTTVGNKIFFFKDRFFWLKVSERPKTSVNLISSLWPTLPSGIEAAYEIEARNQVFLFKDDKYWLISNLRPEPNYPKSIHSFGFPNFVKKIDAAVFNPRFYRTYFFVDNQYWRYDERRQMMDPGYPKLITKNFQGIGPKIDAVFYSKNKYYYFFQGSNQFEYDFLLQRITKTLKSNSWFGC
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P39900 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| Y113 | Phosphorylation | Uniprot | |
| Y116 | Phosphorylation | Uniprot | |
| Y121 | Phosphorylation | Uniprot | |
| Y414 | Phosphorylation | Uniprot | |
| Y434 | Phosphorylation | Uniprot |
研究背景
May be involved in tissue injury and remodeling. Has significant elastolytic activity. Can accept large and small amino acids at the P1' site, but has a preference for leucine. Aromatic or hydrophobic residues are preferred at the P1 site, with small hydrophobic residues (preferably alanine) occupying P3.
Secreted>Extracellular space>Extracellular matrix.
Found in alveolar macrophages but not in peripheral blood monocytes.
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
Belongs to the peptidase M10A family.
文献引用
Application: WB Species: Mouse Sample: NR8383 cells
限制条款
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