产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF9116, RRID:AB_2843306.
展开/折叠
CDC42 binding protein kinase beta; CDC42-binding protein kinase beta; CDC42BPB; DMPK like beta; DMPK-like beta; KIAA1124; MRCK beta; MRCKB_HUMAN; Myotonic dystrophy kinase related CDC42 binding kinase beta; Myotonic dystrophy kinase-related CDC42-binding kinase beta; Myotonic dystrophy protein kinase like beta; Myotonic dystrophy protein kinase-like beta; Serine/threonine protein kinase MRCK beta; Serine/threonine-protein kinase MRCK beta;
抗原和靶标
Expressed in all tissues examined, with high levels in heart, brain, placenta and lung.
- Q9Y5S2 MRCKB_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSAKVRLKKLEQLLLDGPWRNESALSVETLLDVLVCLYTECSHSALRRDKYVAEFLEWAKPFTQLVKEMQLHREDFEIIKVIGRGAFGEVAVVKMKNTERIYAMKILNKWEMLKRAETACFREERDVLVNGDCQWITALHYAFQDENHLYLVMDYYVGGDLLTLLSKFEDKLPEDMARFYIGEMVLAIDSIHQLHYVHRDIKPDNVLLDVNGHIRLADFGSCLKMNDDGTVQSSVAVGTPDYISPEILQAMEDGMGKYGPECDWWSLGVCMYEMLYGETPFYAESLVETYGKIMNHEERFQFPSHVTDVSEEAKDLIQRLICSRERRLGQNGIEDFKKHAFFEGLNWENIRNLEAPYIPDVSSPSDTSNFDVDDDVLRNTEILPPGSHTGFSGLHLPFIGFTFTTESCFSDRGSLKSIMQSNTLTKDEDVQRDLEHSLQMEAYERRIRRLEQEKLELSRKLQESTQTVQSLHGSSRALSNSNRDKEIKKLNEEIERLKNKIADSNRLERQLEDTVALRQEREDSTQRLRGLEKQHRVVRQEKEELHKQLVEASERLKSQAKELKDAHQQRKLALQEFSELNERMAELRAQKQKVSRQLRDKEEEMEVATQKVDAMRQEMRRAEKLRKELEAQLDDAVAEASKERKLREHSENFCKQMESELEALKVKQGGRGAGATLEHQQEISKIKSELEKKVLFYEEELVRREASHVLEVKNVKKEVHDSESHQLALQKEILMLKDKLEKSKRERHNEMEEAVGTIKDKYERERAMLFDENKKLTAENEKLCSFVDKLTAQNRQLEDELQDLAAKKESVAHWEAQIAEIIQWVSDEKDARGYLQALASKMTEELEALRSSSLGSRTLDPLWKVRRSQKLDMSARLELQSALEAEIRAKQLVQEELRKVKDANLTLESKLKDSEAKNRELLEEMEILKKKMEEKFRADTGLKLPDFQDSIFEYFNTAPLAHDLTFRTSSASEQETQAPKPEASPSMSVAASEQQEDMARPPQRPSAVPLPTTQALALAGPKPKAHQFSIKSFSSPTQCSHCTSLMVGLIRQGYACEVCSFACHVSCKDGAPQVCPIPPEQSKRPLGVDVQRGIGTAYKGHVKVPKPTGVKKGWQRAYAVVCDCKLFLYDLPEGKSTQPGVIASQVLDLRDDEFSVSSVLASDVIHATRRDIPCIFRVTASLLGAPSKTSSLLILTENENEKRKWVGILEGLQSILHKNRLRNQVVHVPLEAYDSSLPLIKAILTAAIVDADRIAVGLEEGLYVIEVTRDVIVRAADCKKVHQIELAPREKIVILLCGRNHHVHLYPWSSLDGAEGSFDIKLPETKGCQLMATATLKRNSGTCLFVAVKRLILCYEIQRTKPFHRKFNEIVAPGSVQCLAVLRDRLCVGYPSGFCLLSIQGDGQPLNLVNPNDPSLAFLSQQSFDALCAVELESEEYLLCFSHMGLYVDPQGRRARAQELMWPAAPVACSCSPTHVTVYSEYGVDVFDVRTMEWVQTIGLRRIRPLNSEGTLNLLNCEPPRLIYFKSKFSGAVLNVPDTSDNSKKQMLRTRSKRRFVFKVPEEERLQQRREMLRDPELRSKMISNPTNFNHVAHMGPGDGMQVLMDLPLSAVPPSQEERPGPAPTNLARQPPSRNKPYISWPSSGGSEPSVTVPLRSMSDPDQDFDKEPDSDSTKHSTPSNSSNPSGPPSPNSPHRSQLPLEGLEQPACDT
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q9Y5S2 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S304 | Phosphorylation | Uniprot | |
| T307 | Phosphorylation | Uniprot | |
| S310 | Phosphorylation | Uniprot | |
| K314 | Ubiquitination | Uniprot | |
| K337 | Ubiquitination | Uniprot | |
| S363 | Phosphorylation | Uniprot | |
| S417 | Phosphorylation | Uniprot | |
| S421 | Phosphorylation | Uniprot | |
| T423 | Phosphorylation | Uniprot | |
| K426 | Ubiquitination | Uniprot | |
| K454 | Ubiquitination | Uniprot | |
| K460 | Ubiquitination | Uniprot | |
| T467 | Phosphorylation | Uniprot | |
| S474 | Phosphorylation | Uniprot | |
| S481 | Phosphorylation | Uniprot | |
| K489 | Acetylation | Uniprot | |
| S524 | Phosphorylation | Uniprot | |
| K571 | Ubiquitination | Uniprot | |
| S641 | Phosphorylation | Uniprot | |
| K642 | Ubiquitination | Uniprot | |
| S659 | Phosphorylation | Uniprot | |
| K665 | Ubiquitination | Uniprot | |
| R671 | Methylation | Uniprot | |
| T676 | Phosphorylation | Uniprot | |
| S707 | Phosphorylation | Uniprot | |
| T757 | Phosphorylation | Uniprot | |
| K789 | Ubiquitination | Uniprot | |
| K807 | Ubiquitination | Uniprot | |
| S840 | Phosphorylation | Uniprot | |
| T843 | Phosphorylation | Uniprot | |
| S851 | Phosphorylation | Uniprot | |
| S852 | Phosphorylation | Uniprot | |
| T858 | Phosphorylation | Uniprot | |
| K864 | Ubiquitination | Uniprot | |
| S868 | Phosphorylation | Uniprot | |
| K870 | Ubiquitination | Uniprot | |
| K890 | Ubiquitination | Uniprot | |
| K910 | Ubiquitination | Uniprot | |
| S914 | Phosphorylation | Uniprot | |
| K929 | Ubiquitination | Uniprot | |
| Y954 | Phosphorylation | Uniprot | |
| S1029 | Phosphorylation | Uniprot | |
| K1083 | Ubiquitination | Uniprot | |
| K1099 | Ubiquitination | Uniprot | |
| Y1118 | Phosphorylation | Uniprot | |
| K1135 | Ubiquitination | Uniprot | |
| T1196 | Phosphorylation | Uniprot | |
| K1202 | Ubiquitination | Uniprot | |
| K1280 | Ubiquitination | Uniprot | |
| K1337 | Methylation | Uniprot | |
| K1337 | Ubiquitination | Uniprot | |
| Y1355 | Phosphorylation | Uniprot | |
| K1526 | Methylation | Uniprot | |
| K1528 | Methylation | Uniprot | |
| K1528 | Ubiquitination | Uniprot | |
| S1530 | Phosphorylation | Uniprot | |
| K1544 | Ubiquitination | Uniprot | |
| K1559 | Ubiquitination | Uniprot | |
| Y1638 | Phosphorylation | Uniprot | |
| S1640 | Phosphorylation | Uniprot | |
| S1643 | Phosphorylation | Uniprot | |
| S1644 | Phosphorylation | Uniprot | |
| S1647 | Phosphorylation | Uniprot | |
| S1650 | Phosphorylation | Uniprot | |
| T1652 | Phosphorylation | Uniprot | |
| S1659 | Phosphorylation | Uniprot | |
| S1677 | Phosphorylation | Uniprot | |
| T1678 | Phosphorylation | Uniprot | |
| S1680 | Phosphorylation | Uniprot | |
| S1682 | Phosphorylation | Uniprot | |
| S1686 | Phosphorylation | Uniprot | |
| S1690 | Phosphorylation | Uniprot | |
| S1693 | Phosphorylation | Uniprot | |
| C1709 | S-Nitrosylation | Uniprot |
翻译修饰 - Q9Y5S2 作为激酶
研究背景
Serine/threonine-protein kinase which is an important downstream effector of CDC42 and plays a role in the regulation of cytoskeleton reorganization and cell migration. Regulates actin cytoskeletal reorganization via phosphorylation of PPP1R12C and MYL9/MLC2. In concert with MYO18A and LURAP1, is involved in modulating lamellar actomyosin retrograde flow that is crucial to cell protrusion and migration. Phosphorylates PPP1R12A. In concert with FAM89B/LRAP25 mediates the targeting of LIMK1 to the lamellipodium resulting in its activation and subsequent phosphorylation of CFL1 which is important for lamellipodial F-actin regulation (By similarity).
Proteolytically cleaved by caspases upon apoptosis induction.
Cytoplasm. Cell membrane>Peripheral membrane protein>Cytoplasmic side. Cell junction. Cell projection>Lamellipodium.
Note: Displays a dispersed punctate distribution and concentrates along the cell periphery, especially at the leading edge and cell-cell junction. This concentration is PH-domain dependent (By similarity). Detected at the leading edge of migrating cells. Localization at the leading edge of migrating cells requires interaction with catalytically active CDC42 (PubMed:21240187). Localizes in the lamellipodium in a FAM89B/LRAP25-dependent manner (By similarity).
Expressed in all tissues examined, with high levels in heart, brain, placenta and lung.
Homodimer and homotetramer via the coiled coil regions. Interacts tightly with GTP-bound but not GDP-bound CDC42. Interacts with TJP1, when in the presence of catalytically active CDC42 (By similarity). Forms a tripartite complex with MYO18A and LURAP1 with the latter acting as an adapter connecting CDC42BPB and MYO18A. LURAP1 binding results in activation of CDC42BPB by abolition of its negative autoregulation. Interacts with STRIP1, STRN3 and SIKE1. Interacts with CPNE4 (via VWFA domain). Interacts with LURAP1. Interacts (via AGC-kinase C-terminal domain) with FAM89B/LRAP25 (via LRR repeat). Forms a tripartite complex with FAM89B/LRAP25 and LIMK1 (By similarity).
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. DMPK subfamily.
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