产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF12181, RRID:AB_2844986.
展开/折叠
FLJ14848; hVPS 33B; hVPS33B; Vacuolar protein sorting 33 homolog B (yeast); Vacuolar protein sorting 33 homolog B; Vacuolar protein sorting 33B (yeast); Vacuolar protein sorting 33B; Vacuolar protein sorting associated protein 33B; VPS 33B;
抗原和靶标
- Q9H267 VP33B_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAFPHRPDAPELPDFSMLKRLARDQLIYLLEQLPGKKDLFIEADLMSPLDRIANVSILKQHEVDKLYKVENKPALSSNEQLCFLVRPRIKNMRYIASLVNADKLAGRTRKYKVIFSPQKFYACEMVLEEEGIYGDVSCDEWAFSLLPLDVDLLSMELPEFFRDYFLEGDQRWINTVAQALHLLSTLYGPFPNCYGIGRCAKMAYELWRNLEEEEDGETKGRRPEIGHIFLLDRDVDFVTALCSQVVYEGLVDDTFRIKCGSVDFGPEVTSSDKSLKVLLNAEDKVFNEIRNEHFSNVFGFLSQKARNLQAQYDRRRGMDIKQMKNFVSQELKGLKQEHRLLSLHIGACESIMKKKTKQDFQELIKTEHALLEGFNIRESTSYIEEHIDRQVSPIESLRLMCLLSITENGLIPKDYRSLKTQYLQSYGPEHLLTFSNLRRAGLLTEQAPGDTLTAVESKVSKLVTDKAAGKITDAFSSLAKRSNFRAISKKLNLIPRVDGEYDLKVPRDMAYVFGGAYVPLSCRIIEQVLERRSWQGLDEVVRLLNCSDFAFTDMTKEDKASSESLRLILVVFLGGCTFSEISALRFLGREKGYRFIFLTTAVTNSARLMEAMSEVKA
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q9H267 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| A2 | Acetylation | Uniprot | |
| K19 | Ubiquitination | Uniprot | |
| K36 | Ubiquitination | Uniprot | |
| S56 | Phosphorylation | Uniprot | |
| K65 | Ubiquitination | Uniprot | |
| Y67 | Phosphorylation | Uniprot | |
| K103 | Ubiquitination | Uniprot | |
| K201 | Ubiquitination | Uniprot | |
| T218 | Phosphorylation | Uniprot | |
| K219 | Ubiquitination | Uniprot | |
| K258 | Ubiquitination | Uniprot | |
| K304 | Ubiquitination | Uniprot | |
| Y312 | Phosphorylation | Uniprot | |
| K321 | Ubiquitination | Uniprot | |
| K332 | Ubiquitination | Uniprot | |
| K357 | Ubiquitination | Uniprot | |
| T444 | Phosphorylation | Uniprot | |
| K458 | Ubiquitination | Uniprot | |
| K466 | Ubiquitination | Uniprot | |
| K470 | Ubiquitination | Uniprot | |
| K480 | Ubiquitination | Uniprot | |
| Y501 | Phosphorylation | Uniprot | |
| K504 | Ubiquitination | Uniprot | |
| S547 | Phosphorylation | Uniprot | |
| T552 | Phosphorylation | Uniprot | |
| K616 | Ubiquitination | Uniprot |
研究背景
May play a role in vesicle-mediated protein trafficking to lysosomal compartments and in membrane docking/fusion reactions of late endosomes/lysosomes. Mediates phagolysosomal fusion in macrophages. Proposed to be involved in endosomal maturation implicating VIPAS39. In epithelial cells, the VPS33B:VIPAS39 complex may play a role in the apical recycling pathway and in the maintenance of the apical-basolateral polarity. Seems to be involved in the sorting of specific cargos from the trans-Golgi network to alpha-granule-destined multivesicular bodies (MVBs) promoting MVBs maturation in megakaryocytes (By similarity).
Phosphorylated on tyrosine residues. Dephosphorylation by M.tuberculosis PtpA is necessary to induce the reduction of host phagolysosome fusion in M.tuberculosis-infected macrophages.
Late endosome membrane>Peripheral membrane protein>Cytoplasmic side. Lysosome membrane>Peripheral membrane protein>Cytoplasmic side. Early endosome. Cytoplasmic vesicle>Clathrin-coated vesicle. Recycling endosome.
Note: Colocalizes in clusters with VIPAS39 at cytoplasmic organelles (PubMed:19109425). Colocalizes with RAB11A and VIPAS39 on recycling endosomes (PubMed:22753090). Colocalizes with AP-3, clathrin, Rab5 and Rab7b (PubMed:21411634). Colocalizes with M.tuberculosis PtpA in the cytosol of tuberculosis-infected macrophages and associates with phagosomes (PubMed:18474358).
Ubiquitous; highly expressed in testis and low expression in the lung.
Interacts with RAB11A and VIPAS39. Associates with adaptor protein complex 3 (AP-3), clathrin:AP-3 and clathrin:HGS complexes. Interacts with M.tuberculosis PtpA.
Belongs to the STXBP/unc-18/SEC1 family.
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