| 产品: | CPNE1 抗体 |
| 货号: | DF12912 |
| 描述: | Rabbit polyclonal antibody to CPNE1 |
| 应用: | WB |
| 反应: | Human |
| 预测: | Dog |
| 分子量: | 59 kDa; 59kD(Calculated). |
| 蛋白号: | Q99829 |
| RRID: | AB_2845873 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF12912, RRID:AB_2845873.
展开/折叠
Copine 1; Copine I; Copine-1; Copine1; CopineI; COPN 1; COPN1; CPN 1; CPN1; CPNE 1; CPNE1; CPNE1_HUMAN; MGC1142;
抗原和靶标
Expressed in neutrophils (at protein level) (PubMed:12949241). Widely expressed. Expressed in the brain. Expressed in neutrophil precursors from bone marrow and peripheral blood (PubMed:12949241).
- Q99829 CPNE1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAHCVTLVQLSISCDHLIDKDIGSKSDPLCVLLQDVGGGSWAELGRTERVRNCSSPEFSKTLQLEYRFETVQKLRFGIYDIDNKTPELRDDDFLGGAECSLGQIVSSQVLTLPLMLKPGKPAGRGTITVSAQELKDNRVVTMEVEARNLDKKDFLGKSDPFLEFFRQGDGKWHLVYRSEVIKNNLNPTWKRFSVPVQHFCGGNPSTPIQVQCSDYDSDGSHDLIGTFHTSLAQLQAVPAEFECIHPEKQQKKKSYKNSGTIRVKICRVETEYSFLDYVMGGCQINFTVGVDFTGSNGDPSSPDSLHYLSPTGVNEYLMALWSVGSVVQDYDSDKLFPAFGFGAQVPPDWQVSHEFALNFNPSNPYCAGIQGIVDAYRQALPQVRLYGPTNFAPIINHVARFAAQAAHQGTASQYFMLLLLTDGAVTDVEATREAVVRASNLPMSVIIVGVGGADFEAMEQLDADGGPLHTRSGQAAARDIVQFVPYRRFQNAPREALAQTVLAEVPTQLVSYFRAQGWAPLKPLPPSAKDPAQAPQA
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q99829 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K20 | Ubiquitination | Uniprot | |
| K25 | Ubiquitination | Uniprot | |
| S40 | Phosphorylation | Uniprot | |
| S54 | Phosphorylation | Uniprot | |
| S55 | Phosphorylation | Uniprot | |
| K60 | Ubiquitination | Uniprot | |
| K73 | Ubiquitination | Uniprot | |
| K84 | Sumoylation | Uniprot | |
| K84 | Ubiquitination | Uniprot | |
| T85 | Phosphorylation | Uniprot | |
| K117 | Ubiquitination | Uniprot | |
| K120 | Ubiquitination | Uniprot | |
| T128 | Phosphorylation | Uniprot | |
| S130 | Phosphorylation | Uniprot | |
| K135 | Acetylation | Uniprot | |
| K135 | Sumoylation | Uniprot | |
| K135 | Ubiquitination | Uniprot | |
| K151 | Ubiquitination | Uniprot | |
| K152 | Sumoylation | Uniprot | |
| K157 | Ubiquitination | Uniprot | |
| S158 | Phosphorylation | Uniprot | |
| K171 | Acetylation | Uniprot | |
| K171 | Ubiquitination | Uniprot | |
| K182 | Ubiquitination | Uniprot | |
| K190 | Methylation | Uniprot | |
| K190 | Ubiquitination | Uniprot | |
| K248 | Ubiquitination | Uniprot | |
| S254 | Phosphorylation | Uniprot | |
| K256 | Ubiquitination | Uniprot | |
| T260 | Phosphorylation | Uniprot | |
| Y386 | Phosphorylation | Uniprot | |
| T389 | Phosphorylation | Uniprot | |
| T500 | Phosphorylation | Uniprot | |
| K522 | Ubiquitination | Uniprot | |
| K529 | Ubiquitination | Uniprot |
研究背景
Calcium-dependent phospholipid-binding protein that plays a role in calcium-mediated intracellular processes. Involved in the TNF-alpha receptor signaling pathway in a calcium-dependent manner. Exhibits calcium-dependent phospholipid binding properties. Plays a role in neuronal progenitor cell differentiation; induces neurite outgrowth via a AKT-dependent signaling cascade and calcium-independent manner. May recruit target proteins to the cell membrane in a calcium-dependent manner. May function in membrane trafficking. Involved in TNF-alpha-induced NF-kappa-B transcriptional repression by inducing endoprotease processing of the transcription factor NF-kappa-B p65/RELA subunit. Also induces endoprotease processing of NF-kappa-B p50/NFKB1, p52/NFKB2, RELB and REL.
Nucleus. Cytoplasm. Cell membrane.
Note: Translocates to the cell membrane in a calcium-dependent manner (PubMed:21087455, PubMed:25450385).
Expressed in neutrophils (at protein level). Widely expressed. Expressed in the brain. Expressed in neutrophil precursors from bone marrow and peripheral blood.
Homodimer; homodimerizes via its C2 domains. Interacts with p65/RELA (via N-terminus); this interaction induces proteolytic cleavage of p65/RELA subunit and inhibition of NF-kappa-B transcriptional activity. Interacts (via VWFA domain) with ACTB, CCDC22, MYCBP2, PPP5C, RDX and UBE2O.
C2 domains are necessary for calcium-dependent cell membrane association. C2 domains are necessary for neuronal progenitor cell differentiation in a calcium-independent manner (PubMed:25450385).
Belongs to the copine family.
限制条款
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