产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF13738, RRID:AB_2846757.
展开/折叠
CBP; Csk binding protein; Csk-binding protein; FLJ37858; MGC138364; PAG 1; PAG; PAG1; PAG1_HUMAN; Phosphoprotein associated with glycosphingolipid enriched microdomains 1; Phosphoprotein associated with glycosphingolipid enriched microdomains; Phosphoprotein associated with glycosphingolipid microdomains 1; Phosphoprotein associated with glycosphingolipid-enriched microdomains 1; Protein Associated with Glycosphingolipid Enriched Microdomains; Transmembrane adapter protein PAG; Transmembrane phosphoprotein Cbp;
抗原和靶标
Ubiquitously expressed. Present in germinal center B-cells, plasma cells, T-cells, monocytes and platelets (at protein level).
- Q9NWQ8 PHAG1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MGPAGSLLGSGQMQITLWGSLAAVAIFFVITFLIFLCSSCDREKKPRQHSGDHENLMNVPSDKEMFSRSVTSLATDAPASSEQNGALTNGDILSEDSTLTCMQHYEEVQTSASDLLDSQDSTGKPKCHQSRELPRIPPESAVDTMLTARSVDGDQGLGMEGPYEVLKDSSSQENMVEDCLYETVKEIKEVAAAAHLEKGHSGKAKSTSASKELPGPQTEGKAEFAEYASVDRNKKCRQSVNVESILGNSCDPEEEAPPPVPVKLLDENENLQEKEGGEAEESATDTTSETNKRFSSLSYKSREEDPTLTEEEISAMYSSVNKPGQLVNKSGQSLTVPESTYTSIQGDPQRSPSSCNDLYATVKDFEKTPNSTLPPAGRPSEEPEPDYEAIQTLNREEEKATLGTNGHHGLVPKENDYESISDLQQGRDITRL
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q9NWQ8 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S50 | Phosphorylation | Uniprot | |
| S61 | Phosphorylation | Uniprot | |
| K63 | Ubiquitination | Uniprot | |
| S67 | Phosphorylation | Uniprot | |
| S69 | Phosphorylation | Uniprot | |
| S72 | Phosphorylation | Uniprot | |
| S80 | Phosphorylation | Uniprot | |
| S97 | Phosphorylation | Uniprot | |
| T98 | Phosphorylation | Uniprot | |
| Y105 | Phosphorylation | P07948 (LYN) | Uniprot |
| S150 | Phosphorylation | Uniprot | |
| Y163 | Phosphorylation | Uniprot | |
| S170 | Phosphorylation | Uniprot | |
| S171 | Phosphorylation | Uniprot | |
| Y181 | Phosphorylation | Uniprot | |
| T183 | Phosphorylation | Uniprot | |
| Y227 | Phosphorylation | Uniprot | |
| S229 | Phosphorylation | Uniprot | |
| S239 | Phosphorylation | Uniprot | |
| S288 | Phosphorylation | Uniprot | |
| S295 | Phosphorylation | Uniprot | |
| S296 | Phosphorylation | Uniprot | |
| S298 | Phosphorylation | Uniprot | |
| Y299 | Phosphorylation | Uniprot | |
| S301 | Phosphorylation | Uniprot | |
| T307 | Phosphorylation | Uniprot | |
| S314 | Phosphorylation | Uniprot | |
| Y317 | Phosphorylation | P12931 (SRC) | Uniprot |
| S318 | Phosphorylation | Uniprot | |
| S319 | Phosphorylation | Uniprot | |
| S339 | Phosphorylation | Uniprot | |
| T340 | Phosphorylation | Uniprot | |
| Y341 | Phosphorylation | Uniprot | |
| T342 | Phosphorylation | Uniprot | |
| S343 | Phosphorylation | Uniprot | |
| S351 | Phosphorylation | Uniprot | |
| S353 | Phosphorylation | Uniprot | |
| S354 | Phosphorylation | Uniprot | |
| Y359 | Phosphorylation | Uniprot | |
| T361 | Phosphorylation | Uniprot | |
| S380 | Phosphorylation | Uniprot | |
| Y387 | Phosphorylation | Uniprot | |
| T392 | Phosphorylation | Uniprot | |
| T404 | Phosphorylation | Uniprot | |
| Y417 | Phosphorylation | Uniprot | |
| S419 | Phosphorylation | Uniprot | |
| S421 | Phosphorylation | Uniprot |
研究背景
Negatively regulates TCR (T-cell antigen receptor)-mediated signaling in T-cells and FCER1 (high affinity immunoglobulin epsilon receptor)-mediated signaling in mast cells. Promotes CSK activation and recruitment to lipid rafts, which results in LCK inhibition. Inhibits immunological synapse formation by preventing dynamic arrangement of lipid raft proteins. May be involved in cell adhesion signaling.
Palmitoylated.
Phosphorylated by FYN on Tyr-317 in resting T-cells; which promotes interaction with CSK. Dephosphorylated by PTPRC/CD45 upon TCR activation; which leads to CSK dissociation. May also be dephosphorylated by PTPN11. Hyperphosphorylated in mast cells upon FCER1 activation. Phosphorylated by LYN.
Cell membrane>Single-pass type III membrane protein.
Note: Present in lipid rafts.
Ubiquitously expressed. Present in germinal center B-cells, plasma cells, T-cells, monocytes and platelets (at protein level).
Interacts with FYN. When phosphorylated, interacts with CSK. Interacts with SLC9A3R1/EBP50. In resting T-cells, part of a PAG1-SLC9A3R1-MSN complex which is disrupted upon TCR activation. Interacts with LYN on plasma membrane lipid rafts. Identified in a complex with LYN and STAT3.
限制条款
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