产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF3040, RRID:AB_2835423.
展开/折叠
BERP; Brain expressed ring finger; Brain-expressed RING finger protein; FLJ16135; HAC1; RING finger protein 22; RING finger protein 97; RNF22; RNF97; Trim3; TRIM3_HUMAN; Tripartite motif containing 3; Tripartite motif protein TRIM3; Tripartite motif-containing protein 3;
抗原和靶标
- O75382 TRIM3_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAKREDSPGPEVQPMDKQFLVCSICLDRYQCPKVLPCLHTFCERCLQNYIPAQSLTLSCPVCRQTSILPEQGVSALQNNFFISSLMEAMQQAPDGAHDPEDPHPLSVVAGRPLSCPNHEGKTMEFYCEACETAMCGECRAGEHREHGTVLLRDVVEQHKAALQRQLEAVRGRLPQLSAAIALVGGISQQLQERKAEALAQISAAFEDLEQALQQRKQALVSDLETICGAKQKVLQSQLDTLRQGQEHIGSSCSFAEQALRLGSAPEVLLVRKHMRERLAALAAQAFPERPHENAQLELVLEVDGLRRSVLNLGALLTTSATAHETVATGEGLRQALVGQPASLTVTTKDKDGRLVRTGSAELRAEITGPDGTRLPVPVVDHKNGTYELVYTARTEGELLLSVLLYGQPVRGSPFRVRALRPGDLPPSPDDVKRRVKSPGGPGSHVRQKAVRRPSSMYSTGGKRKDNPIEDELVFRVGSRGREKGEFTNLQGVSAASSGRIVVADSNNQCIQVFSNEGQFKFRFGVRGRSPGQLQRPTGVAVDTNGDIIVADYDNRWVSIFSPEGKFKTKIGAGRLMGPKGVAVDRNGHIIVVDNKSCCVFTFQPNGKLVGRFGGRGATDRHFAGPHFVAVNNKNEIVVTDFHNHSVKVYSADGEFLFKFGSHGEGNGQFNAPTGVAVDSNGNIIVADWGNSRIQVFDSSGSFLSYINTSAEPLYGPQGLALTSDGHVVVADAGNHCFKAYRYLQ
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - O75382 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| A2 | Acetylation | Uniprot | |
| S7 | Phosphorylation | Uniprot | |
| S23 | Phosphorylation | Uniprot | |
| K159 | Ubiquitination | Uniprot | |
| S202 | Phosphorylation | Uniprot | |
| K216 | Ubiquitination | Uniprot | |
| S359 | Phosphorylation | Uniprot | |
| S412 | Phosphorylation | Uniprot | |
| S427 | Phosphorylation | Uniprot | |
| S437 | Phosphorylation | Uniprot | |
| S443 | Phosphorylation | Uniprot | |
| S454 | Phosphorylation | Uniprot | |
| S455 | Phosphorylation | Uniprot | |
| Y457 | Phosphorylation | Uniprot | |
| S458 | Phosphorylation | Uniprot | |
| K483 | Ubiquitination | Uniprot | |
| K579 | Ubiquitination | Uniprot | |
| R615 | Methylation | Uniprot | |
| T639 | Phosphorylation | Uniprot |
研究背景
Probably involved in vesicular trafficking via its association with the CART complex. The CART complex is necessary for efficient transferrin receptor recycling but not for EGFR degradation. Positively regulates motility of microtubule-dependent motor protein KIF21B (By similarity).
Cytoplasm. Early endosome. Golgi apparatus>trans-Golgi network. Cell projection>Dendrite.
Expressed in brain, heart, uterus and testis.
Associates with myosin-Vb (MYO5B) and alpha-actinin-4 (ACTN4) (By similarity). Component of the CART complex, at least composed of ACTN4, HGS/HRS, MYO5B and TRIM3. Interacts with ZFYVE28/LST2. Interacts with KIF21B (By similarity).
The interaction with MYO5B is dependent upon its NHL repeats, which form a beta-propeller (NHL) domain containing six blades.
Belongs to the TRIM/RBCC family.
限制条款
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