产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF3150, RRID:AB_2835527.
展开/折叠
cAMP dependent protein kinase type I beta regulatory subunit; cAMP-dependent protein kinase type I-beta regulatory subunit; KAP1_HUMAN; PKARI beta; PRKAR 1; PRKAR 1B; PRKAR1; Prkar1b; PRKAR1B protein; Protein kinase cAMP dependent regulatory type I beta; RIbeta;
抗原和靶标
Four types of regulatory chains are found: I-alpha, I-beta, II-alpha, and II-beta. Their expression varies among tissues and is in some cases constitutive and in others inducible.
- P31321 KAP1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MASPPACPSEEDESLKGCELYVQLHGIQQVLKDCIVHLCISKPERPMKFLREHFEKLEKEENRQILARQKSNSQSDSHDEEVSPTPPNPVVKARRRRGGVSAEVYTEEDAVSYVRKVIPKDYKTMTALAKAISKNVLFAHLDDNERSDIFDAMFPVTHIAGETVIQQGNEGDNFYVVDQGEVDVYVNGEWVTNISEGGSFGELALIYGTPRAATVKAKTDLKLWGIDRDSYRRILMGSTLRKRKMYEEFLSKVSILESLEKWERLTVADALEPVQFEDGEKIVVQGEPGDDFYIITEGTASVLQRRSPNEEYVEVGRLGPSDYFGEIALLLNRPRAATVVARGPLKCVKLDRPRFERVLGPCSEILKRNIQRYNSFISLTV
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P31321 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S3 | Phosphorylation | Uniprot | |
| S9 | Phosphorylation | Uniprot | |
| S14 | Phosphorylation | Uniprot | |
| S71 | Phosphorylation | Uniprot | |
| S73 | Phosphorylation | Uniprot | |
| S75 | Phosphorylation | Uniprot | |
| S77 | Phosphorylation | Uniprot | |
| S83 | Phosphorylation | Uniprot | |
| T85 | Phosphorylation | Uniprot | |
| K92 | Ubiquitination | Uniprot | |
| Y207 | Phosphorylation | Uniprot | |
| K222 | Ubiquitination | Uniprot | |
| S238 | Phosphorylation | Uniprot | |
| T239 | Phosphorylation | Uniprot | |
| K244 | Ubiquitination | Uniprot | |
| Y246 | Phosphorylation | Uniprot | |
| S251 | Phosphorylation | Uniprot | |
| K261 | Ubiquitination | Uniprot | |
| Y312 | Phosphorylation | Uniprot | |
| T338 | Phosphorylation | Uniprot | |
| R342 | Methylation | Uniprot | |
| K346 | Ubiquitination | Uniprot | |
| K349 | Ubiquitination | Uniprot |
研究背景
Regulatory subunit of the cAMP-dependent protein kinases involved in cAMP signaling in cells.
The pseudophosphorylation site binds to the substrate-binding region of the catalytic chain, resulting in the inhibition of its activity.
Cell membrane.
Four types of regulatory chains are found: I-alpha, I-beta, II-alpha, and II-beta. Their expression varies among tissues and is in some cases constitutive and in others inducible.
The inactive holoenzyme is composed of two regulatory chains and two catalytic chains. Activation by cAMP releases the two active catalytic monomers and the regulatory dimer. Interacts with PRKX; regulates this cAMP-dependent protein kinase. Interacts with C2orf88/smAKAP; this interaction may target PRKAR1B to the plasma membrane.
Belongs to the cAMP-dependent kinase regulatory chain family.
研究领域
· Organismal Systems > Endocrine system > Insulin signaling pathway. (View pathway)
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