产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF3259, RRID:AB_2835557.
展开/折叠
CDAN4; EKLF; EKLF PEN; Erythroid krueppel like transcription factor (EKLF) (Erythroid transcription factor); Erythroid krueppel-like transcription factor; Erythroid Kruppel like factor; Erythroid specific transcription factor EKLF; HBFQTL6; Human erythroid-specific transcription factor EKLF mRNA complete cds; INLU; Klf1; KLF1_HUMAN; Krueppel-like factor 1; Kruppel like factor 1 (erythroid); Basic transcription element binding protein 2; Basic transcription element-binding protein 2; BTE binding protein 2; BTE-binding protein 2; BTEB 2; BTEB2; CKLF; Colon krueppel like factor; Colon krueppel-like factor; GC box binding protein 2; GC-box-binding protein 2; IKLF; Intestinal enriched krueppel like factor; Intestinal-enriched krueppel-like factor; KLF 5; Klf5; KLF5_HUMAN; Krueppel like factor 5; Krueppel-like factor 5; Kruppel like factor 5 intestinal; Transcription factor BTEB 2; Transcription factor BTEB2; KLF7; KLF7_HUMAN; Krueppel like factor 7; Krueppel-like factor 7; Kruppel like factor 7; Ubiquitous krueppel like factor; Ubiquitous krueppel-like factor; UKLF;
抗原和靶标
Expression restricted to adult bone marrow and fetal liver. Not expressed in myeloid nor lymphoid cell lines.
Q13887 KLF5_HUMAN:Expressed only in testis and placenta.
O75840 KLF7_HUMAN:Widely expressed.
- Q13351 KLF1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MATAETALPSISTLTALGPFPDTQDDFLKWWRSEEAQDMGPGPPDPTEPPLHVKSEDQPGEEEDDERGADATWDLDLLLTNFSGPEPGGAPQTCALAPSEASGAQYPPPPETLGAYAGGPGLVAGLLGSEDHSGWVRPALRARAPDAFVGPALAPAPAPEPKALALQPVYPGPGAGSSGGYFPRTGLSVPAASGAPYGLLSGYPAMYPAPQYQGHFQLFRGLQGPAPGPATSPSFLSCLGPGTVGTGLGGTAEDPGVIAETAPSKRGRRSWARKRQAAHTCAHPGCGKSYTKSSHLKAHLRTHTGEKPYACTWEGCGWRFARSDELTRHYRKHTGQRPFRCQLCPRAFSRSDHLALHMKRHL
- Q13887 KLF5_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MATRVLSMSARLGPVPQPPAPQDEPVFAQLKPVLGAANPARDAALFPGEELKHAHHRPQAQPAPAQAPQPAQPPATGPRLPPEDLVQTRCEMEKYLTPQLPPVPIIPEHKKYRRDSASVVDQFFTDTEGLPYSINMNVFLPDITHLRTGLYKSQRPCVTHIKTEPVAIFSHQSETTAPPPAPTQALPEFTSIFSSHQTAAPEVNNIFIKQELPTPDLHLSVPTQQGHLYQLLNTPDLDMPSSTNQTAAMDTLNVSMSAAMAGLNTHTSAVPQTAVKQFQGMPPCTYTMPSQFLPQQATYFPPSPPSSEPGSPDRQAEMLQNLTPPPSYAATIASKLAIHNPNLPTTLPVNSQNIQPVRYNRRSNPDLEKRRIHYCDYPGCTKVYTKSSHLKAHLRTHTGEKPYKCTWEGCDWRFARSDELTRHYRKHTGAKPFQCGVCNRSFSRSDHLALHMKRHQN
- O75840 KLF7_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MDVLASYSIFQELQLVHDTGYFSALPSLEETWQQTCLELERYLQTEPRRISETFGEDLDCFLHASPPPCIEESFRRLDPLLLPVEAAICEKSSAVDILLSRDKLLSETCLSLQPASSSLDSYTAVNQAQLNAVTSLTPPSSPELSRHLVKTSQTLSAVDGTVTLKLVAKKAALSSVKVGGVATAAAAVTAAGAVKSGQSDSDQGGLGAEACPENKKRVHRCQFNGCRKVYTKSSHLKAHQRTHTGEKPYKCSWEGCEWRFARSDELTRHYRKHTGAKPFKCNHCDRCFSRSDHLALHMKRHI
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q13351/Q13887/O75840 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| T15 | Phosphorylation | Uniprot | |
| T23 | Phosphorylation | P17612 (PRKACA) | Uniprot |
| K265 | Acetylation | Uniprot | |
| K274 | Acetylation | Uniprot | |
| K288 | Acetylation | Uniprot | |
| K297 | Sumoylation | Uniprot | |
| K297 | Ubiquitination | Uniprot | |
| T302 | Phosphorylation | Uniprot | |
| T304 | Phosphorylation | Uniprot |
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K31 | Ubiquitination | Uniprot | |
| K110 | Sumoylation | Uniprot | |
| K110 | Ubiquitination | Uniprot | |
| S116 | Phosphorylation | Uniprot | |
| S153 | Phosphorylation | Q05655 (PRKCD) | Uniprot |
| K162 | Sumoylation | Uniprot | |
| K209 | Sumoylation | Uniprot | |
| S303 | Phosphorylation | P49841 (GSK3B) | Uniprot |
| T323 | Phosphorylation | Uniprot | |
| T346 | Phosphorylation | Uniprot | |
| Y359 | Phosphorylation | Uniprot | |
| S363 | Phosphorylation | Uniprot | |
| K369 | Acetylation | Uniprot | |
| Y377 | Phosphorylation | Uniprot | |
| K391 | Sumoylation | Uniprot | |
| K391 | Ubiquitination | Uniprot | |
| T396 | Phosphorylation | Uniprot | |
| T398 | Phosphorylation | Uniprot | |
| K401 | Ubiquitination | Uniprot | |
| S441 | Phosphorylation | Uniprot | |
| S443 | Phosphorylation | Uniprot |
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| T45 | Phosphorylation | Uniprot | |
| T189 | Phosphorylation | Uniprot | |
| S196 | Phosphorylation | Uniprot | |
| S199 | Phosphorylation | Uniprot | |
| S201 | Phosphorylation | Uniprot | |
| T242 | Phosphorylation | Uniprot | |
| T244 | Phosphorylation | Uniprot | |
| K247 | Ubiquitination | Uniprot |
研究背景
Transcription regulator of erythrocyte development that probably serves as a general switch factor during erythropoiesis. Is a dual regulator of fetal-to-adult globin switching. Binds to the CACCC box in the beta-globin gene promoter and acts as a preferential activator of this gene. Furthermore, it binds to the BCL11A promoter and activates expression of BCL11A, which in turn represses the HBG1 and HBG2 genes. This dual activity ensures that, in most adults, fetal hemoglobin levels are low. Able to activate CD44 and AQP1 promoters. When sumoylated, acts as a transcriptional repressor by promoting interaction with CDH2/MI2beta and also represses megakaryocytic differentiation.
Acetylated; can be acetylated on both Lys-274 and Lys-288. Acetylation on Lys-274 (by CBP) appears to be the major site affecting EKLF transactivation activity (By similarity).
Sumoylated; sumoylation, promoted by PIAS1, leads to repression of megakaryocyte differentiation. Also promotes the interaction with the CDH4 subunit of the NuRD repression complex (By similarity).
Phosphorylated primarily on serine residues in the transactivation domain. Phosphorylation on Thr-23 is critical for the transactivation activity (By similarity).
Nucleus.
Note: Colocalizes with SUMO1 in nuclear speckles.
Expression restricted to adult bone marrow and fetal liver. Not expressed in myeloid nor lymphoid cell lines.
Interacts with PCAF; the interaction does not acetylate EKLF and inhibits its transactivation activity (By similarity). Interacts with CREBBP/CBP and EP300; the interactions enhance the transactivation activity. Interacts with TFB1.
The 9aaTAD motif is a transactivation domain present in a large number of yeast and animal transcription factors.
Belongs to the krueppel C2H2-type zinc-finger protein family.
Transcription factor that binds to GC box promoter elements. Activates the transcription of these genes.
Ubiquitinated. Polyubiquitination involves WWP1 and leads to proteasomal degradation of this protein.
Nucleus.
Expressed only in testis and placenta.
Interacts with WWP1.
The 9aaTAD motif is a transactivation domain present in a large number of yeast and animal transcription factors.
Belongs to the krueppel C2H2-type zinc-finger protein family.
Transcriptional factor. Plays a critical role in neuronal morphogenesis and survival of sensory neurons (By similarity). Represses the corneal epithelium differentiation. Acts also as a metabolic regulator, by modulating insulin sensitivity in pancreatic beta cells and skeletal muscle cells. Inhibits transcriptional inducers of adipogenesis and has a repressive role in the expression of several adipokines, including leptin.
Nucleus.
Widely expressed.
Interacts with FBXO38.
The acidic N-terminal part may favor interaction with the basic domain of transcription factors.
The 9aaTAD motif is a transactivation domain present in a large number of yeast and animal transcription factors. In KLF7, the motif is inactive.
Belongs to the krueppel C2H2-type zinc-finger protein family.
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