产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF4786, RRID:AB_2837140.
展开/折叠
A930014J19Rik; CDC42 binding protein kinase alpha (DMPK like); CDC42 binding protein kinase alpha; CDC42 binding protein kinase alpha dmpk like; CDC42 binding protein kinase beta; CDC42-binding protein kinase alpha; CDC42BPA; DKFZp686L1738; DKFZp686P1738; DMPK like; DMPK-like alpha; FLJ23347; KIAA0451; MGC189823; MRCK alpha; mrck; mrcka; MRCKA_HUMAN; Myotonic dystrophy kinase related CDC42 binding protein kinase alpha; Myotonic dystrophy kinase-related CDC42-binding kinase alpha; Myotonic dystrophy protein kinase-like alpha; OTTHUMP00000035727; OTTHUMP00000035728; OTTHUMP00000035729; OTTHUMP00000035730; pk428; RP5-1087E8.4; Ser thr protein kinase PK428; Ser thr protein kinase related to the myotonic dystrophy protein kinase; Serine/threonine protein kinase MRCK alpha; Serine/threonine-protein kinase MRCK alpha;
抗原和靶标
Abundant in the heart, brain, skeletal muscle, kidney, and pancreas, with little or no expression in the lung and liver.
- Q5VT25 MRCKA_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSGEVRLRQLEQFILDGPAQTNGQCFSVETLLDILICLYDECNNSPLRREKNILEYLEWAKPFTSKVKQMRLHREDFEILKVIGRGAFGEVAVVKLKNADKVFAMKILNKWEMLKRAETACFREERDVLVNGDNKWITTLHYAFQDDNNLYLVMDYYVGGDLLTLLSKFEDRLPEDMARFYLAEMVIAIDSVHQLHYVHRDIKPDNILMDMNGHIRLADFGSCLKLMEDGTVQSSVAVGTPDYISPEILQAMEDGKGRYGPECDWWSLGVCMYEMLYGETPFYAESLVETYGKIMNHKERFQFPAQVTDVSENAKDLIRRLICSREHRLGQNGIEDFKKHPFFSGIDWDNIRNCEAPYIPEVSSPTDTSNFDVDDDCLKNSETMPPPTHTAFSGHHLPFVGFTYTSSCVLSDRSCLRVTAGPTSLDLDVNVQRTLDNNLATEAYERRIKRLEQEKLELSRKLQESTQTVQALQYSTVDGPLTASKDLEIKNLKEEIEKLRKQVTESSHLEQQLEEANAVRQELDDAFRQIKAYEKQIKTLQQEREDLNKELVQASERLKNQSKELKDAHCQRKLAMQEFMEINERLTELHTQKQKLARHVRDKEEEVDLVMQKVESLRQELRRTERAKKELEVHTEALAAEASKDRKLREQSEHYSKQLENELEGLKQKQISYSPGVCSIEHQQEITKLKTDLEKKSIFYEEELSKREGIHANEIKNLKKELHDSEGQQLALNKEIMILKDKLEKTRRESQSEREEFESEFKQQYEREKVLLTEENKKLTSELDKLTTLYENLSIHNQQLEEEVKDLADKKESVAHWEAQITEIIQWVSDEKDARGYLQALASKMTEELEALRNSSLGTRATDMPWKMRRFAKLDMSARLELQSALDAEIRAKQAIQEELNKVKASNIITECKLKDSEKKNLELLSEIEQLIKDTEELRSEKGIEHQDSQHSFLAFLNTPTDALDQFERSPSCTPASKGRRTVDSTPLSVHTPTLRKKGCPGSTGFPPKRKTHQFFVKSFTTPTKCHQCTSLMVGLIRQGCSCEVCGFSCHITCVNKAPTTCPVPPEQTKGPLGIDPQKGIGTAYEGHVRIPKPAGVKKGWQRALAIVCDFKLFLYDIAEGKASQPSVVISQVIDMRDEEFSVSSVLASDVIHASRKDIPCIFRVTASQLSASNNKCSILMLADTENEKNKWVGVLSELHKILKKNKFRDRSVYVPKEAYDSTLPLIKTTQAAAIIDHERIALGNEEGLFVVHVTKDEIIRVGDNKKIHQIELIPNDQLVAVISGRNRHVRLFPMSALDGRETDFYKLSETKGCQTVTSGKVRHGALTCLCVAMKRQVLCYELFQSKTRHRKFKEIQVPYNVQWMAIFSEQLCVGFQSGFLRYPLNGEGNPYSMLHSNDHTLSFIAHQPMDAICAVEISSKEYLLCFNSIGIYTDCQGRRSRQQELMWPANPSSCCYNAPYLSVYSENAVDIFDVNSMEWIQTLPLKKVRPLNNEGSLNLLGLETIRLIYFKNKMAEGDELVVPETSDNSRKQMVRNINNKRRYSFRVPEEERMQQRREMLRDPEMRNKLISNPTNFNHIAHMGPGDGIQILKDLPMNPRPQESRTVFSGSVSIPSITKSRPEPGRSMSASSGLSARSSAQNGSALKREFSGGSYSAKRQPMPSPSEGSLSSGGMDQGSDAPARDFDGEDSDSPRHSTASNSSNLSSPPSPASPRKTKSLSLESTDRGSWDP
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q5VT25 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S2 | Phosphorylation | Uniprot | |
| R172 | Methylation | Uniprot | |
| R179 | Methylation | Uniprot | |
| S222 | Phosphorylation | Q5VT25 (CDC42BPA) | Uniprot |
| S234 | Phosphorylation | Q5VT25 (CDC42BPA) | Uniprot |
| T240 | Phosphorylation | Q5VT25 (CDC42BPA) | Uniprot |
| K338 | Ubiquitination | Uniprot | |
| K339 | Ubiquitination | Uniprot | |
| T403 | Phosphorylation | Q5VT25 (CDC42BPA) | Uniprot |
| Y444 | Phosphorylation | Uniprot | |
| K455 | Ubiquitination | Uniprot | |
| K461 | Ubiquitination | Uniprot | |
| Y474 | Phosphorylation | Uniprot | |
| S475 | Phosphorylation | Uniprot | |
| K490 | Ubiquitination | Uniprot | |
| K531 | Ubiquitination | Uniprot | |
| K669 | Acetylation | Uniprot | |
| K669 | Ubiquitination | Uniprot | |
| S674 | Phosphorylation | Uniprot | |
| S679 | Phosphorylation | Uniprot | |
| T687 | Phosphorylation | Uniprot | |
| S725 | Phosphorylation | Uniprot | |
| S750 | Phosphorylation | Uniprot | |
| K785 | Ubiquitination | Uniprot | |
| S843 | Phosphorylation | Uniprot | |
| T846 | Phosphorylation | Uniprot | |
| S856 | Phosphorylation | Uniprot | |
| T862 | Phosphorylation | Uniprot | |
| K893 | Ubiquitination | Uniprot | |
| K913 | Ubiquitination | Uniprot | |
| K920 | Ubiquitination | Uniprot | |
| S940 | Phosphorylation | Uniprot | |
| S989 | Phosphorylation | Uniprot | |
| T992 | Phosphorylation | Uniprot | |
| T994 | Phosphorylation | Uniprot | |
| S1003 | Phosphorylation | Uniprot | |
| K1079 | Ubiquitination | Uniprot | |
| K1122 | Ubiquitination | Uniprot | |
| S1171 | Phosphorylation | Uniprot | |
| S1173 | Phosphorylation | Uniprot | |
| T1185 | Phosphorylation | Uniprot | |
| K1217 | Ubiquitination | Uniprot | |
| K1228 | Ubiquitination | Uniprot | |
| K1307 | Ubiquitination | Uniprot | |
| K1312 | Ubiquitination | Uniprot | |
| S1378 | Phosphorylation | Uniprot | |
| T1505 | Phosphorylation | Uniprot | |
| Y1510 | Phosphorylation | Uniprot | |
| Y1544 | Phosphorylation | Uniprot | |
| S1545 | Phosphorylation | Uniprot | |
| S1604 | Phosphorylation | Uniprot | |
| S1611 | Phosphorylation | Uniprot | |
| S1613 | Phosphorylation | Uniprot | |
| S1616 | Phosphorylation | Uniprot | |
| T1618 | Phosphorylation | Uniprot | |
| S1629 | Phosphorylation | Uniprot | |
| S1632 | Phosphorylation | Uniprot | |
| S1635 | Phosphorylation | Uniprot | |
| S1639 | Phosphorylation | Uniprot | |
| S1644 | Phosphorylation | Uniprot | |
| S1651 | Phosphorylation | Uniprot | |
| S1654 | Phosphorylation | Uniprot | |
| S1656 | Phosphorylation | Uniprot | |
| S1664 | Phosphorylation | Uniprot | |
| S1669 | Phosphorylation | Uniprot | |
| S1693 | Phosphorylation | Uniprot | |
| S1700 | Phosphorylation | Uniprot | |
| S1703 | Phosphorylation | Uniprot | |
| S1706 | Phosphorylation | Uniprot | |
| S1710 | Phosphorylation | Uniprot | |
| S1719 | Phosphorylation | Uniprot | |
| S1721 | Phosphorylation | Uniprot | |
| S1724 | Phosphorylation | Uniprot | |
| T1725 | Phosphorylation | Uniprot | |
| S1729 | Phosphorylation | Uniprot |
翻译修饰 - Q5VT25 作为激酶
| Substrate | Site | Source |
|---|---|---|
| P10916 (MYL2) | S19 | Uniprot |
| P24844 (MYL9) | T19 | Uniprot |
| P24844 (MYL9) | S20 | Uniprot |
| P26038 (MSN) | T558 | Uniprot |
| P53667-1 (LIMK1) | T508 | Uniprot |
| P53671-2 (LIMK2) | T505 | Uniprot |
| P53671-1 (LIMK2) | T526 | Uniprot |
| Q5VT25 (CDC42BPA) | S222 | Uniprot |
| Q5VT25 (CDC42BPA) | S234 | Uniprot |
| Q5VT25 (CDC42BPA) | T240 | Uniprot |
| Q5VT25 (CDC42BPA) | T403 | Uniprot |
| Q9BZL4 (PPP1R12C) | T560 | Uniprot |
研究背景
Serine/threonine-protein kinase which is an important downstream effector of CDC42 and plays a role in the regulation of cytoskeleton reorganization and cell migration. Regulates actin cytoskeletal reorganization via phosphorylation of PPP1R12C and MYL9/MLC2. In concert with MYO18A and LURAP1, is involved in modulating lamellar actomyosin retrograde flow that is crucial to cell protrusion and migration. Phosphorylates: PPP1R12A, LIMK1 and LIMK2. May play a role in TFRC-mediated iron uptake. In concert with FAM89B/LRAP25 mediates the targeting of LIMK1 to the lamellipodium resulting in its activation and subsequent phosphorylation of CFL1 which is important for lamellipodial F-actin regulation (By similarity). Triggers the formation of an extrusion apical actin ring required for epithelial extrusion of apoptotic cells.
Proteolytically cleaved by caspases upon apoptosis induction. The cleavage at Asp-478 by CASP3 increases its kinase activity (in vitro).
Cytoplasm. Cell projection>Lamellipodium.
Note: Displays a dispersed punctate distribution and concentrates along the cell periphery, especially at the leading edge and cell-cell junction. This concentration is PH-domain dependent. Localizes in the lamellipodium in a FAM89B/LRAP25-dependent manner.
Abundant in the heart, brain, skeletal muscle, kidney, and pancreas, with little or no expression in the lung and liver.
Homodimer and homotetramer via the coiled coil regions. Interacts tightly with GTP-bound but not GDP-bound CDC42. Forms a tripartite complex with MYO18A and LURAP1 with the latter acting as an adapter connecting CDC42BPA and MYO18A. LURAP1 binding results in activation of CDC42BPA by abolition of its negative autoregulation. Interacts with LURAP1. Interacts (via AGC-kinase C-terminal domain) with FAM89B/LRAP25 (via LRR repeat). Forms a tripartite complex with FAM89B/LRAP25 and LIMK1 (By similarity).
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. DMPK subfamily.
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