产品: ZDHHC13 抗体
货号: DF2600
描述: Rabbit polyclonal antibody to ZDHHC13
应用: WB IHC
反应: Human, Monkey
预测: Pig, Bovine, Horse, Sheep, Rabbit, Dog
分子量: 58 kDa; 71kD(Calculated).
蛋白号: Q8IUH4
RRID: AB_2839806

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Monkey
预测:
Pig(90%), Bovine(90%), Horse(90%), Sheep(90%), Rabbit(90%), Dog(90%)
克隆:
Polyclonal
特异性:
ZDHHC13 Antibody detects endogenous levels of total ZDHHC13.
RRID:
AB_2839806
引用格式: Affinity Biosciences Cat# DF2600, RRID:AB_2839806.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

DHHC 13; DHHC-13; FLJ10852; FLJ10941; HIP14 like; HIP14 related protein; HIP14-related protein; HIP14L; HIP3RP; Huntingtin interacting protein 14 related protein; Huntingtin interacting protein HIP3RP; Huntingtin-interacting protein 14-related protein; Huntingtin-interacting protein HIP3RP; Hypothetical protein similar to ankyrin repeat containing priotein AKR1; MGC64994; Palmitoyltransferase ZDHHC13; Probable palmitoyltransferase ZDHHC13; Putative MAPK activating protein PM03; Putative MAPK-activating protein PM03; Putative NF kappa B activating protein 209; Putative NF-kappa-B-activating protein 209; ZDH13_HUMAN; ZDHHC 13; ZDHHC13; Zinc finger DHHC domain containing 13; Zinc finger DHHC domain-containing protein 13;

抗原和靶标

免疫原:
Uniprot:
基因/基因ID:
描述:
Palmitoyltransferase for HD and GAD2 (By similarity). Mediates Mg(2+) transport.
序列:
MEGPGLGSQCRNHSHGPHPPGFGRYGICAHENKELANAREALPLIEDSSNCDIVKATQYGIFERCKELVEAGYDVRQPDKENVSLLHWAAINNRLDLVKFYISKGAVVDQLGGDLNSTPLHWAIRQGHLPMVILLLQHGADPTLIDGEGFSSIHLAVLFQHMPIIAYLISKGQSVNMTDVNGQTPLMLSAHKVIGPEPTGFLLKFNPSLNVVDKIHQNTPLHWAVAAGNVNAVDKLLEAGSSLDIQNVKGETPLDMALQNKNQLIIHMLKTEAKMRANQKFRLWRWLQKCELFLLLMLSVITMWAIGYILDFNSDSWLLKGCLLVTLFFLTSLFPRFLVGYKNLVYLPTAFLLSSVFWIFMTWFILFFPDLAGAPFYFSFIFSIVAFLYFFYKTWATDPGFTKASEEEKKVNIITLAETGSLDFRTFCTSCLIRKPLRSLHCHVCNCCVARYDQHCLWTGRCIGFGNHHYYIFFLFFLSMVCGWIIYGSFIYLSSHCATTFKEDGLWTYLNQIVACSPWVLYILMLATFHFSWSTFLLLNQLFQIAFLGLTSHERISLQKQSKHMKQTLSLRKTPYNLGFMQNLADFFQCGCFGLVKPCVVDWTSQYTMVFHPAREKVLRSV

种属预测

种属预测:

score>80的预测可信度较高,可尝试用于WB检测。*预测模型主要基于免疫原序列比对,结果仅作参考,不作为质保凭据。

Species
Results
Score
Pig
90
Horse
90
Bovine
90
Sheep
90
Dog
90
Rabbit
90
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - Q8IUH4 作为底物

Site PTM Type Enzyme
M1 Acetylation
S8 Phosphorylation
Y25 Phosphorylation
K33 Ubiquitination
K55 Ubiquitination
Y59 Phosphorylation
K66 Ubiquitination
Y73 Phosphorylation
R76 Methylation
K80 Ubiquitination
K192 Ubiquitination
K214 Ubiquitination
K235 Ubiquitination
K249 Ubiquitination
K261 Ubiquitination
K566 Ubiquitination

研究背景

功能:

Palmitoyltransferase for HTT and GAD2. May play a role in Mg(2+) transport.

细胞定位:

Golgi apparatus membrane>Multi-pass membrane protein. Cytoplasmic vesicle membrane>Multi-pass membrane protein.
Note: Low extracellular Mg(2+) induces increase in Golgi and in post-Golgi vesicles.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
亚基结构:

Interacts (via ANK repeats) with CLIP3. Interacts (via ANK repeats) with DNAJC5 (via C-terminus) (By similarity). Interacts (via ANK repeats) with HTT. Interacts (via ANK repeats) with MAP6. Interacts (via ANK repeats) with SNAP23. Interacts (via ANK repeats) with SNAP25. May interact (via ANK repeats) with SPRED2 (By similarity).

蛋白家族:

The DHHC domain is required for palmitoyltransferase activity.

Belongs to the DHHC palmitoyltransferase family. AKR/ZDHHC17 subfamily.

文献引用

1). A peptidic inhibitor for PD-1 palmitoylation targets its expression and functions. RSC Chemical Biology, 2021 (PubMed: 34458782) [IF=4.1]

Application: WB    Species: Human    Sample: A375 cells

Fig. 3 PD-1 is palmitoylated by DHHC9. (A) Western blot showing the effect of two independent siRNAs targeting the indicated DHHC enzymes on DHHC protein levels in A375 cells with the specific antibody for each DHHC enzyme. (B) Western blot showing the expression of PD-1 in A375 cells treated by two independent siRNAs targeting the indicated DHHC enzymes. (C) Western blot showing the expression of PD-1 in RKO and HCT116 cells treated by two independent siRNAs targeting DHHC9. Statistics shown on the right, *** P o 0.001, ** P o 0.01, ANOVA test. n = 3 independent experiments. (D) Immunoblot showing the expression of PD-1 in different cells overexpressing DHHC9. Statistics shown on the right, *** P o 0.001, ** P o 0.01, ANOVA test. n = 3 independent experiments.

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