GPR55 Antibody - #DF2752

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产品: GPR55 抗体
货号: DF2752
描述: Rabbit polyclonal antibody to GPR55
应用: WB IHC
反应: Human
预测: Bovine, Horse, Sheep, Rabbit, Dog
分子量: 37kDa; 37kD(Calculated).
蛋白号: Q9Y2T6
RRID: AB_2839958

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

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MS Report
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WB Handbook
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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human
预测:
Bovine(80%), Horse(89%), Sheep(90%), Rabbit(90%), Dog(80%)
克隆:
Polyclonal
特异性:
GPR55 Antibody detects endogenous levels of total GPR55.
RRID:
AB_2839958
引用格式: Affinity Biosciences Cat# DF2752, RRID:AB_2839958.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

G protein coupled receptor 55; G-protein coupled receptor 55; GPCR GPR55; GPR 55; Gpr55; GPR55_HUMAN; LPIR1; Probable G protein coupled receptor 55;

抗原和靶标

免疫原:
Uniprot:

Q9Y2T6(GPR55_HUMAN) >>Visit HPA database.

基因/基因ID:
GPR55(9290)
表达:
Q9Y2T6 GPR55_HUMAN:

Expressed in the caudate nucleus and putamen, but not detected in the hippocampus, thalamus, pons cerebellum, frontal cortex of the brain or in the liver. Expressed in osteoclasts and osteoblasts.

描述:
May be involved in hyperalgesia associated with inflammatory and neuropathic pain (By similarity). Receptor for L-alpha-lysophosphatidylinositol (LPI). LPI induces Ca(2+) release from intracellular stores via the heterotrimeric G protein GNA13 and RHOA. Putative cannabinoid receptor. May play a role in bone physiology by regulating osteoclast number and function.
序列:
MSQQNTSGDCLFDGVNELMKTLQFAVHIPTFVLGLLLNLLAIHGFSTFLKNRWPDYAATSIYMINLAVFDLLLVLSLPFKMVLSQVQSPFPSLCTLVECLYFVSMYGSVFTICFISMDRFLAIRYPLLVSHLRSPRKIFGICCTIWVLVWTGSIPIYSFHGKVEKYMCFHNMSDDTWSAKVFFPLEVFGFLLPMGIMGFCCSRSIHILLGRRDHTQDWVQQKACIYSIAASLAVFVVSFLPVHLGFFLQFLVRNSFIVECRAKQSISFFLQLSMCFSNVNCCLDVFCYYFVIKEFRMNIRAHRPSRVQLVLQDTTISRG

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Rabbit
90
Sheep
90
Horse
89
Bovine
80
Dog
80
Chicken
67
Pig
0
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

功能:

May be involved in hyperalgesia associated with inflammatory and neuropathic pain (By similarity). Receptor for L-alpha-lysophosphatidylinositol (LPI). LPI induces Ca(2+) release from intracellular stores via the heterotrimeric G protein GNA13 and RHOA. Putative cannabinoid receptor. May play a role in bone physiology by regulating osteoclast number and function.

细胞定位:

Cell membrane>Multi-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Expressed in the caudate nucleus and putamen, but not detected in the hippocampus, thalamus, pons cerebellum, frontal cortex of the brain or in the liver. Expressed in osteoclasts and osteoblasts.

蛋白家族:

Belongs to the G-protein coupled receptor 1 family.

文献引用

1). The GPR55 antagonist CID16020046 mitigates advanced glycation end products (AGEs)-induced chondrocyte activation. CHEMICO-BIOLOGICAL INTERACTIONS, 2020 (PubMed: 32360554) [IF=5.1]

Application: WB    Species: human    Sample: ATDC5 cells

Fig. 1. |Advanced Glycation End Products(AGEs) increased the expression of GPR55 in human ATDC5 cells. Cells were stimulated with 50, 100, and 200 μg/ml AGEs for 24 h. (A). Real time PCR analysis revealed that AGEs increased the expression of GPR55 at the mRNA levels; (B). Western blot analysis revealed that AGEs increased the expression of GPR55 at the protein levels (*, **, ***, P < 0.05, 0.01, 0.005 vs.previous group, n = 4).

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