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| 产品: | 磷酸化 MRLC1 (Thr19+Ser20) 抗体 |
| 货号: | AF8010 |
| 描述: | Rabbit polyclonal antibody to Phospho-MRLC1 (Thr19+Ser20) |
| 应用: | WB |
| 反应: | Human, Mouse, Rat |
| 预测: | Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus |
| 分子量: | 18kDa; 20kD(Calculated). |
| 蛋白号: | P24844 |
| RRID: | AB_2840073 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF8010, RRID:AB_2840073.
展开/折叠
20 kDa myosin light chain;Human 20kDa myosin light chain (MLC2) mRNA complete cds;LC20;MGC3505;MLC 2;MLC-2C;MLC2;MLY 9;MRLC1;MYL9;MYL9_HUMAN;Myosin light chain 9 regulatory;Myosin light polypeptide 9 regulatory;myosin regulatory light chain 1;Myosin regulatory light chain 2;Myosin regulatory light chain 2 smooth muscle isoform;Myosin regulatory light chain 9;Myosin regulatory light chain MRLC1;Myosin regulatory light polypeptide 9;Myosin RLC;Myosin vascular smooth muscle light chain 2;MYRL2;OTTHUMP00000030857;smooth muscle isoform; ML12B_HUMAN;MLC 2A;MLC-2;MLC-2a;MLC-B;MLC20;MRLC2;MYL12B;MYLC2B;Myosin light chain 12B regulatory;Myosin regulatory light chain 12B;myosin regulatory light chain 2;Myosin regulatory light chain 2-B;myosin regulatory light chain 2-B, smooth muscle isoform;Myosin regulatory light chain 20 kDa;Myosin regulatory light chain MRLC2;myosin, light chain 12B, regulatory;OTTHUMP00000162244;OTTHUMP00000165806;OTTHUMP00000165807;OTTHUMP00000165808;SHUJUN-1;smooth muscle isoform;
抗原和靶标
- P24844 MYL9_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSSKRAKAKTTKKRPQRATSNVFAMFDQSQIQEFKEAFNMIDQNRDGFIDKEDLHDMLASLGKNPTDEYLEGMMSEAPGPINFTMFLTMFGEKLNGTDPEDVIRNAFACFDEEASGFIHEDHLRELLTTMGDRFTDEEVDEMYREAPIDKKGNFNYVEFTRILKHGAKDKDD
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P24844 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S2 | Phosphorylation | P17252 (PRKCA) | Uniprot |
| S3 | Phosphorylation | P17252 (PRKCA) | Uniprot |
| T10 | Phosphorylation | P17252 (PRKCA) | Uniprot |
| T11 | Phosphorylation | Uniprot | |
| T19 | Phosphorylation | O43293 (DAPK3) , O14578 (CIT) , Q15746 (MYLK) , Q5VT25 (CDC42BPA) , Q13464 (ROCK1) | Uniprot |
| S20 | Phosphorylation | O43293 (DAPK3) , Q5VT25 (CDC42BPA) , O75116 (ROCK2) , Q14012 (CAMK1) , Q15746 (MYLK) , Q13153 (PAK1) , Q13177 (PAK2) , Q9Y5S2 (CDC42BPB) , O14578 (CIT) , Q13464 (ROCK1) | Uniprot |
| S29 | Phosphorylation | Uniprot | |
| K51 | Acetylation | Uniprot | |
| K51 | Ubiquitination | Uniprot | |
| S60 | Phosphorylation | Uniprot | |
| T97 | Phosphorylation | Uniprot | |
| S115 | Phosphorylation | Uniprot | |
| T128 | Phosphorylation | Uniprot | |
| T135 | Phosphorylation | Uniprot | |
| Y143 | Phosphorylation | P00533 (EGFR) | Uniprot |
| K150 | Ubiquitination | Uniprot | |
| K151 | Sumoylation | Uniprot | |
| K151 | Ubiquitination | Uniprot | |
| Y156 | Phosphorylation | P00533 (EGFR) | Uniprot |
| K164 | Ubiquitination | Uniprot | |
| K168 | Acetylation | Uniprot |
研究背景
Myosin regulatory subunit that plays an important role in regulation of both smooth muscle and nonmuscle cell contractile activity via its phosphorylation. Implicated in cytokinesis, receptor capping, and cell locomotion.
Phosphorylation increases the actin-activated myosin ATPase activity and thereby regulates the contractile activity. It is required to generate the driving force in the migration of the cells but not necessary for localization of myosin-2 at the leading edge.
Smooth muscle tissues and in some, but not all, nonmuscle cells.
Myosin is a hexamer of 2 heavy chains and 4 light chains: interacts with myosin heavy chain MYO19.
研究领域
· Cellular Processes > Cellular community - eukaryotes > Focal adhesion. (View pathway)
· Cellular Processes > Cellular community - eukaryotes > Tight junction. (View pathway)
· Cellular Processes > Cell motility > Regulation of actin cytoskeleton. (View pathway)
· Environmental Information Processing > Signal transduction > cGMP-PKG signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > cAMP signaling pathway. (View pathway)
· Organismal Systems > Circulatory system > Vascular smooth muscle contraction. (View pathway)
· Organismal Systems > Immune system > Leukocyte transendothelial migration. (View pathway)
· Organismal Systems > Endocrine system > Oxytocin signaling pathway.
文献引用
Application: WB Species: rat Sample: Intestinal
Application: WB Species: Mice Sample: mRMVECs
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