产品: 磷酸化 RAD50 (Ser635) 抗体
货号: AF8308
描述: Rabbit polyclonal antibody to Phospho-RAD50 (Ser635)
应用: WB IHC
反应: Human, Mouse, Rat
预测: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
分子量: 154kDa; 154kD(Calculated).
蛋白号: Q92878
RRID: AB_2840370

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   规格 价格 库存
 100ul RMB¥ 2800 现货
 200ul RMB¥ 3800 现货

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产品描述

来源:
Rabbit
应用:
WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(100%), Zebrafish(88%), Bovine(100%), Horse(88%), Sheep(100%), Rabbit(100%), Dog(100%), Xenopus(88%)
克隆:
Polyclonal
特异性:
Phospho-RAD50 (Ser635) Antibody detects endogenous levels of RAD50 only when phosphorylated at Ser635.
RRID:
AB_2840370
引用格式: Affinity Biosciences Cat# AF8308, RRID:AB_2840370.
偶联:
Unconjugated.
纯化:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

DNA repair protein RAD50; hRAD50; hsRAD50; Mrell; NBSLD; Rad 50; RAD50 2; rad50; RAD50 homolog (S cerevisiae); RAD50 homolog; RAD50 PEN; RAD50, S. cerevisiae, homolog of; RAD50_HUMAN; RAD502; Rad50l; Truncated RAD50 protein;

抗原和靶标

免疫原:
Uniprot:
基因/基因ID:
表达:
Q92878 RAD50_HUMAN:

Expressed at very low level in most tissues, except in testis where it is expressed at higher level. Expressed in fibroblasts.

序列:
MSRIEKMSILGVRSFGIEDKDKQIITFFSPLTILVGPNGAGKTTIIECLKYICTGDFPPGTKGNTFVHDPKVAQETDVRAQIRLQFRDVNGELIAVQRSMVCTQKSKKTEFKTLEGVITRTKHGEKVSLSSKCAEIDREMISSLGVSKAVLNNVIFCHQEDSNWPLSEGKALKQKFDEIFSATRYIKALETLRQVRQTQGQKVKEYQMELKYLKQYKEKACEIRDQITSKEAQLTSSKEIVKSYENELDPLKNRLKEIEHNLSKIMKLDNEIKALDSRKKQMEKDNSELEEKMEKVFQGTDEQLNDLYHNHQRTVREKERKLVDCHRELEKLNKESRLLNQEKSELLVEQGRLQLQADRHQEHIRARDSLIQSLATQLELDGFERGPFSERQIKNFHKLVRERQEGEAKTANQLMNDFAEKETLKQKQIDEIRDKKTGLGRIIELKSEILSKKQNELKNVKYELQQLEGSSDRILELDQELIKAERELSKAEKNSNVETLKMEVISLQNEKADLDRTLRKLDQEMEQLNHHTTTRTQMEMLTKDKADKDEQIRKIKSRHSDELTSLLGYFPNKKQLEDWLHSKSKEINQTRDRLAKLNKELASSEQNKNHINNELKRKEEQLSSYEDKLFDVCGSQDFESDLDRLKEEIEKSSKQRAMLAGATAVYSQFITQLTDENQSCCPVCQRVFQTEAELQEVISDLQSKLRLAPDKLKSTESELKKKEKRRDEMLGLVPMRQSIIDLKEKEIPELRNKLQNVNRDIQRLKNDIEEQETLLGTIMPEEESAKVCLTDVTIMERFQMELKDVERKIAQQAAKLQGIDLDRTVQQVNQEKQEKQHKLDTVSSKIELNRKLIQDQQEQIQHLKSTTNELKSEKLQISTNLQRRQQLEEQTVELSTEVQSLYREIKDAKEQVSPLETTLEKFQQEKEELINKKNTSNKIAQDKLNDIKEKVKNIHGYMKDIENYIQDGKDDYKKQKETELNKVIAQLSECEKHKEKINEDMRLMRQDIDTQKIQERWLQDNLTLRKRNEELKEVEEERKQHLKEMGQMQVLQMKSEHQKLEENIDNIKRNHNLALGRQKGYEEEIIHFKKELREPQFRDAEEKYREMMIVMRTTELVNKDLDIYYKTLDQAIMKFHSMKMEEINKIIRDLWRSTYRGQDIEYIEIRSDADENVSASDKRRNYNYRVVMLKGDTALDMRGRCSAGQKVLASLIIRLALAETFCLNCGIIALDEPTTNLDRENIESLAHALVEIIKSRSQQRNFQLLVITHDEDFVELLGRSEYVEKFYRIKKNIDQCSEIVKCSVSSLGFNVH

种属预测

种属预测:

score>80的预测可信度较高,可尝试用于WB检测。*预测模型主要基于免疫原序列比对,结果仅作参考,不作为质保凭据。

Species
Results
Score
Pig
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Horse
88
Xenopus
88
Zebrafish
88
Chicken
75
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - Q92878 作为底物

Site PTM Type Enzyme
K6 Ubiquitination
K20 Methylation
K62 Ubiquitination
K71 Ubiquitination
K112 Ubiquitination
K126 Ubiquitination
S128 Phosphorylation
S130 Phosphorylation
K132 Ubiquitination
K175 Ubiquitination
K187 Ubiquitination
R193 Methylation
T198 Phosphorylation
K211 Ubiquitination
K230 Ubiquitination
K238 Ubiquitination
K242 Ubiquitination
K252 Ubiquitination
K256 Ubiquitination
S263 Phosphorylation
K264 Acetylation
K273 Ubiquitination
K280 Ubiquitination
K295 Ubiquitination
Y308 Phosphorylation
K343 Ubiquitination
R359 Methylation
K398 Acetylation
K446 Ubiquitination
K453 Acetylation
K461 Acetylation
K461 Ubiquitination
S470 Phosphorylation
S471 Phosphorylation
R473 Methylation
K483 Ubiquitination
K520 Ubiquitination
K585 Acetylation
K599 Ubiquitination
K616 Ubiquitination
S623 Phosphorylation
S624 Phosphorylation
S635 Phosphorylation Q13315 (ATM) , Q13535 (ATR)
S640 Phosphorylation
T690 Phosphorylation
K743 Ubiquitination
K753 Ubiquitination
R797 Methylation
R823 Methylation
K838 Ubiquitination
K845 Ubiquitination
K851 Ubiquitination
K864 Ubiquitination
K921 Ubiquitination
K959 Acetylation
Y964 Phosphorylation
K982 Ubiquitination
K1032 Acetylation
K1059 Ubiquitination
K1068 Ubiquitination
K1089 Ubiquitination
T1113 Phosphorylation
T1114 Phosphorylation
K1119 Ubiquitination
Y1125 Phosphorylation
K1126 Ubiquitination
K1134 Ubiquitination
Y1162 Phosphorylation
K1178 Ubiquitination
K1190 Ubiquitination
K1285 Acetylation
K1285 Ubiquitination
K1291 Ubiquitination
K1301 Ubiquitination

研究背景

功能:

Component of the MRN complex, which plays a central role in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity and meiosis. The complex possesses single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity, which are provided by MRE11. RAD50 may be required to bind DNA ends and hold them in close proximity. This could facilitate searches for short or long regions of sequence homology in the recombining DNA templates, and may also stimulate the activity of DNA ligases and/or restrict the nuclease activity of MRE11 to prevent nucleolytic degradation past a given point. The complex may also be required for DNA damage signaling via activation of the ATM kinase. In telomeres the MRN complex may modulate t-loop formation.

细胞定位:

Nucleus. Chromosome>Telomere. Chromosome.
Note: Localizes to discrete nuclear foci after treatment with genotoxic agents.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Expressed at very low level in most tissues, except in testis where it is expressed at higher level. Expressed in fibroblasts.

亚基结构:

Component of the MRN complex composed of two heterodimers RAD50/MRE11 associated with a single NBN. As part of the MRN complex, interacts with MCM8 and MCM9; the interaction recruits the complex to DNA repair sites. Component of the BASC complex, at least composed of BRCA1, MSH2, MSH6, MLH1, ATM, BLM, RAD50, MRE11 and NBN. Found in a complex with TERF2. Interacts with RINT1. Interacts with BRCA1 via its N-terminal domain. Interacts with DCLRE1C/Artemis. Interacts with MRNIP.

(Microbial infection) Interacts with herpes simplex virus 1 protein UL12.

蛋白家族:

The zinc-hook, which separates the large intramolecular coiled coil regions, contains 2 Cys residues that coordinate one molecule of zinc with the help of the 2 Cys residues of the zinc-hook of another RAD50 molecule, thereby forming a V-shaped homodimer. The two heads of the homodimer, which constitute the ATP-binding domain, interact with the MRE11 homodimer (By similarity).

Belongs to the SMC family. RAD50 subfamily.

研究领域

· Cellular Processes > Cell growth and death > Cellular senescence.   (View pathway)

· Genetic Information Processing > Replication and repair > Homologous recombination.

· Genetic Information Processing > Replication and repair > Non-homologous end-joining.

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