Phospho-PDHA1/2 (Ser293/Ser291) Antibody - #AF8502

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产品: 磷酸化 PDHA1/2 (Ser293/Ser291) 抗体
货号: AF8502
描述: Rabbit polyclonal antibody to Phospho-PDHA1/2 (Ser293/Ser291)
应用: WB IHC IF/ICC
反应: Human, Mouse, Rat
预测: Pig, Bovine, Horse, Sheep, Rabbit, Chicken, Xenopus
分子量: 43 kDa; 43kD(Calculated).
蛋白号: P08559 | P29803
RRID: AB_2840556

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产品描述

来源:
Rabbit
应用:
WB 1:1000-3000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Chicken(100%), Xenopus(100%)
克隆:
Polyclonal
特异性:
Phospho-PDHA1/2 (Ser293/Ser291) Antibody detects endogenous levels of PDHA1/2 only when phosphorylated at Ser293/291.
RRID:
AB_2840556
引用格式: Affinity Biosciences Cat# AF8502, RRID:AB_2840556.
偶联:
Unconjugated.
纯化:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

ODPA_HUMAN; PDH; PDHA; PDHA1; PDHCE1A; PDHE1 A type I; PDHE1-A type I; PHE1A; Pyruvate Dehydrogenase (lipoamide) alpha 1; Pyruvate dehydrogenase complex, E1 alpha polypeptide 1; Pyruvate Dehydrogenase E1 alpha; Pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial; EC 1.2.4.1; MGC149517; MGC149518; mitochondrial; ODPAT_HUMAN; Pdha2; PDHAL; PDHE1 A type II; PDHE1-A type II; Pyruvate dehydrogenase (lipoamide) alpha 2; Pyruvate dehydrogenase E1 component subunit alpha; Pyruvate dehydrogenase E1 component subunit alpha, testis specific form, mitochondrial; Pyruvate dehydrogenase, E1 alpha polypeptide, testis spec; testis-specific form;

抗原和靶标

免疫原:
Uniprot:

P08559(ODPA_HUMAN) >>Visit HPA database.

P29803(ODPAT_HUMAN) >>Visit HPA database.

基因/基因ID:
PDHA1(5160) | PDHA2(5161)
表达:
P08559 ODPA_HUMAN:

Ubiquitous.

P29803 ODPAT_HUMAN:

Testis. Expressed in postmeiotic spermatogenic cells.

序列:
MRKMLAAVSRVLSGASQKPASRVLVASRNFANDATFEIKKCDLHRLEEGPPVTTVLTREDGLKYYRMMQTVRRMELKADQLYKQKIIRGFCHLCDGQEACCVGLEAGINPTDHLITAYRAHGFTFTRGLSVREILAELTGRKGGCAKGKGGSMHMYAKNFYGGNGIVGAQVPLGAGIALACKYNGKDEVCLTLYGDGAANQGQIFEAYNMAALWKLPCIFICENNRYGMGTSVERAAASTDYYKRGDFIPGLRVDGMDILCVREATRFAAAYCRSGKGPILMELQTYRYHGHSMSDPGVSYRTREEIQEVRSKSDPIMLLKDRMVNSNLASVEELKEIDVEVRKEIEDAAQFATADPEPPLEELGYHIYSSDPPFEVRGANQWIKFKSVS

MLAAFISRVLRRVAQKSARRVLVASRNSSNDATFEIKKCDLYLLEEGPPVTTVLTRAEGLKYYRMMLTVRRMELKADQLYKQKFIRGFCHLCDGQEACCVGLEAGINPSDHVITSYRAHGVCYTRGLSVRSILAELTGRRGGCAKGKGGSMHMYTKNFYGGNGIVGAQGPLGAGIALACKYKGNDEICLTLYGDGAANQGQIAEAFNMAALWKLPCVFICENNLYGMGTSTERAAASPDYYKRGNFIPGLKVDGMDVLCVREATKFAANYCRSGKGPILMELQTYRYHGHSMSDPGVSYRTREEIQEVRSKRDPIIILQDRMVNSKLATVEELKEIGAEVRKEIDDAAQFATTDPEPHLEELGHHIYSSDSSFEVRGANPWIKFKSVS

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Xenopus
100
Chicken
100
Rabbit
100
Dog
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P08559/P29803 作为底物

Site PTM Type Enzyme
K18 Acetylation
K18 Ubiquitination
K39 Acetylation
K39 Ubiquitination
K63 Acetylation
K63 Ubiquitination
K77 Acetylation
K83 Acetylation
K83 Ubiquitination
T116 Phosphorylation
Y118 Phosphorylation
T139 Phosphorylation
S152 Phosphorylation
Y227 Phosphorylation
T231 Phosphorylation
S232 Phosphorylation Q15118 (PDK1) , O15530 (PDPK1) , Q15119 (PDK2)
T240 Phosphorylation
Y242 Phosphorylation
Y243 Phosphorylation
K244 Acetylation
K244 Ubiquitination
R245 Methylation
Y272 Phosphorylation
K277 Acetylation
Y289 Phosphorylation
S293 Phosphorylation Q16654 (PDK4) , Q15119 (PDK2) , O15530 (PDPK1) , Q15120 (PDK3) , Q15118 (PDK1)
S295 Phosphorylation
S300 Phosphorylation Q15119 (PDK2) , Q15118 (PDK1) , Q16654 (PDK4) , Q15120 (PDK3) , O15530 (PDPK1)
Y301 Phosphorylation
T303 Phosphorylation
K313 Acetylation
K321 Acetylation
K321 Ubiquitination
K336 Acetylation
K344 Ubiquitination
Y366 Phosphorylation
Y369 Phosphorylation
K385 Methylation
Site PTM Type Enzyme
K16 Sumoylation
K37 Acetylation
Y42 Phosphorylation
Y62 Phosphorylation
Y63 Phosphorylation
K75 Acetylation
K81 Acetylation
K81 Ubiquitination
S150 Phosphorylation
Y154 Phosphorylation
S230 Phosphorylation Q15118 (PDK1)
K275 Acetylation
Y287 Phosphorylation
S291 Phosphorylation Q15120 (PDK3) , Q15119 (PDK2) , Q15118 (PDK1) , Q16654 (PDK4)
S293 Phosphorylation
S298 Phosphorylation Q16654 (PDK4) , Q15119 (PDK2) , Q15120 (PDK3) , Q15118 (PDK1)
Y299 Phosphorylation
T301 Phosphorylation

研究背景

功能:

The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2), and thereby links the glycolytic pathway to the tricarboxylic cycle.

翻译修饰:

Phosphorylation at Ser-232, Ser-293 and Ser-300 by PDK family kinases inactivates the enzyme; for this phosphorylation at a single site is sufficient. Dephosphorylation at all three sites, i.e. at Ser-232, Ser-293 and Ser-300, is required for reactivation.

Acetylation alters the phosphorylation pattern. Deacetylated by SIRT3 (By similarity).

细胞定位:

Mitochondrion matrix.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Ubiquitous.

亚基结构:

Heterotetramer of two PDHA1 and two PDHB subunits. The heterotetramer interacts with DLAT, and is part of the multimeric pyruvate dehydrogenase complex that contains multiple copies of pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (DLAT, E2) and lipoamide dehydrogenase (DLD, E3). These subunits are bound to an inner core composed of about 48 DLAT and 12 PDHX molecules.

功能:

The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2), and thereby links the glycolytic pathway to the tricarboxylic cycle.

翻译修饰:

Phosphorylation at Ser-291, Ser-293 and Ser-298 by PDK family kinases inactivates the enzyme; for this phosphorylation at a single site is sufficient. Phosphorylation at Ser-293 interferes with access to active site, and thereby inactivates the enzyme. Dephosphorylation at all three sites, i.e. at Ser-291, Ser-293 and Ser-298, is required for reactivation.

细胞定位:

Mitochondrion matrix.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Testis. Expressed in postmeiotic spermatogenic cells.

亚基结构:

Heterotetramer of two PDHA2 and two PDHB subunits. The heterotetramer interacts with DLAT, and is part of the multimeric pyruvate dehydrogenase complex that contains multiple copies of pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (DLAT, E2) and lipoamide dehydrogenase (DLD, E3). These subunits are bound to an inner core composed of about 48 DLAT and 12 PDHX molecules.

研究领域

· Environmental Information Processing > Signal transduction > HIF-1 signaling pathway.   (View pathway)

· Human Diseases > Cancers: Overview > Central carbon metabolism in cancer.   (View pathway)

· Metabolism > Carbohydrate metabolism > Glycolysis / Gluconeogenesis.

· Metabolism > Carbohydrate metabolism > Citrate cycle (TCA cycle).

· Metabolism > Carbohydrate metabolism > Pyruvate metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

· Metabolism > Global and overview maps > Carbon metabolism.

· Organismal Systems > Endocrine system > Glucagon signaling pathway.

文献引用

1). HIF-1 regulates energy metabolism of the Tibetan chicken brain during embryo development under hypoxia. American Journal of Physiology-Regulatory, Integrative and Comparative Physiology (PubMed: 33596720) [IF=2.8]

Application: WB    Species: chicken    Sample:

Figure 5. Protein expression results of related genes during hypoxia at different developmental stages of the Tibetan chicken (TBC) and Dwarf Laying Chicken (DLC) embryonic brain. A–C: Western blot results of related genes in TBCs (A and B) and DLCs (C) under hypoxia as the embryo develops. D: chromatin immunoprecipitation (ChIP) assay of hypoxia-inducible factor-1 (HIF-1) binding to hexokinase 1 (HK1) and lactate dehydrogenase A (LDHA) was performed on the chicken embryonic brain. HTBC, TBCs under hypoxia; HDLC, DLCs under hypoxia. Data are presented as means ± SE.

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