产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF10010, RRID:AB_2840590.
展开/折叠
ERS 24; ERS24; SEC22 vesicle trafficking protein homolog B; SEC22 vesicle trafficking protein like 1; SEC22B; SEC22L 1; Vesicle trafficking protein SEC22b;
抗原和靶标
- O75396 SC22B_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MVLLTMIARVADGLPLAASMQEDEQSGRDLQQYQSQAKQLFRKLNEQSPTRCTLEAGAMTFHYIIEQGVCDLVLCEAAFPKTLAFAYLEDLHSEFDEQHGKKVPTVSRPYSFIEFDTFIQKTKKLYIDSCARRNLGSINTELQDVQRIMVANIEEVLQRGEALSALDSKANNLSSLSKKYRQDAKYLNMHSTYAKLAAVAVFFIMLIVYVRFWWL
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - O75396 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| T5 | Phosphorylation | Uniprot | |
| S19 | Phosphorylation | Uniprot | |
| S26 | Phosphorylation | Uniprot | |
| Y33 | Phosphorylation | Uniprot | |
| S35 | Phosphorylation | Uniprot | |
| K38 | Acetylation | Uniprot | |
| K38 | Ubiquitination | Uniprot | |
| S48 | Phosphorylation | Uniprot | |
| T50 | Phosphorylation | Uniprot | |
| S93 | Phosphorylation | Uniprot | |
| K102 | Ubiquitination | Uniprot | |
| S107 | Phosphorylation | Uniprot | |
| Y110 | Phosphorylation | Uniprot | |
| S111 | Phosphorylation | Uniprot | |
| K121 | Ubiquitination | Uniprot | |
| S137 | Phosphorylation | Uniprot | |
| T140 | Phosphorylation | Uniprot | |
| S164 | Phosphorylation | Uniprot | |
| S168 | Phosphorylation | Uniprot | |
| K169 | Ubiquitination | Uniprot | |
| S174 | Phosphorylation | Uniprot | |
| S175 | Phosphorylation | Uniprot | |
| S177 | Phosphorylation | Uniprot | |
| K178 | Ubiquitination | Uniprot | |
| K179 | Ubiquitination | Uniprot | |
| K185 | Ubiquitination | Uniprot | |
| T192 | Phosphorylation | Uniprot | |
| Y193 | Phosphorylation | Uniprot |
研究背景
SNARE involved in targeting and fusion of ER-derived transport vesicles with the Golgi complex as well as Golgi-derived retrograde transport vesicles with the ER.
Endoplasmic reticulum membrane>Single-pass type IV membrane protein. Endoplasmic reticulum-Golgi intermediate compartment membrane. Golgi apparatus>cis-Golgi network membrane. Golgi apparatus>trans-Golgi network membrane. Melanosome.
Note: Concentrated most in the intermediate compartment/cis-Golgi network and the cis-Golgi cisternae 1 and 2. Greatly reduced in concentration at the trans end of the Golgi apparatus (By similarity). Identified by mass spectrometry in melanosome fractions from stage I to stage IV (PubMed:17081065).
Interacts with STX17 (By similarity). Component of two distinct SNARE complexes consisting of STX5, GOSR2/BOS1, BET1 and SEC22B or STX18, USE1L, BNIP1/SEC20L and SEC22B. YKT6 can probably replace SEC22B in either complex. Interacts with the COPII Sec23/24 complex composed of SEC23A and SEC24A; recruits SEC22B into COPII-coated vesicles to allow its transport from the endoplasmic reticulum to the Golgi.
Belongs to the synaptobrevin family.
研究领域
· Cellular Processes > Transport and catabolism > Phagosome. (View pathway)
· Genetic Information Processing > Folding, sorting and degradation > SNARE interactions in vesicular transport.
· Human Diseases > Infectious diseases: Bacterial > Legionellosis.
限制条款
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