NUMB Antibody - #DF7559
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF7559, RRID:AB_2841053.
展开/折叠
c14_5527; C14orf41; FLJ31314; h Numb; h-Numb; Numb; Numb homolog (Drosophila); Numb homolog; Numb protein homolog; NUMB_HUMAN; Protein numb homolog; Protein S171; S171;
抗原和靶标
- P49757 NUMB_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MNKLRQSFRRKKDVYVPEASRPHQWQTDEEGVRTGKCSFPVKYLGHVEVDESRGMHICEDAVKRLKAERKFFKGFFGKTGKKAVKAVLWVSADGLRVVDEKTKDLIVDQTIEKVSFCAPDRNFDRAFSYICRDGTTRRWICHCFMAVKDTGERLSHAVGCAFAACLERKQKREKECGVTATFDASRTTFTREGSFRVTTATEQAEREEIMKQMQDAKKAETDKIVVGSSVAPGNTAPSPSSPTSPTSDATTSLEMNNPHAIPRRHAPIEQLARQGSFRGFPALSQKMSPFKRQLSLRINELPSTMQRKTDFPIKNAVPEVEGEAESISSLCSQITNAFSTPEDPFSSAPMTKPVTVVAPQSPTFQANGTDSAFHVLAKPAHTALAPVAMPVRETNPWAHAPDAANKEIAATCSGTEWGQSSGAASPGLFQAGHRRTPSEADRWLEEVSKSVRAQQPQASAAPLQPVLQPPPPTAISQPASPFQGNAFLTSQPVPVGVVPALQPAFVPAQSYPVANGMPYPAPNVPVVGITPSQMVANVFGTAGHPQAAHPHQSPSLVRQQTFPHYEASSATTSPFFKPPAQHLNGSAAFNGVDDGRLASADRHTEVPTGTCPVDPFEAQWAALENKSKQRTNPSPTNPFSSDLQKTFEIEL
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P49757 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S7 | Phosphorylation | Uniprot | |
| K12 | Ubiquitination | Uniprot | |
| Y15 | Phosphorylation | Uniprot | |
| S20 | Phosphorylation | Uniprot | |
| T27 | Phosphorylation | Uniprot | |
| K36 | Ubiquitination | Uniprot | |
| R53 | Methylation | Uniprot | |
| K63 | Ubiquitination | Uniprot | |
| K73 | Ubiquitination | Uniprot | |
| S91 | Phosphorylation | Uniprot | |
| T102 | Phosphorylation | Q2M2I8 (AAK1) | Uniprot |
| R121 | Methylation | Uniprot | |
| T187 | Phosphorylation | Uniprot | |
| T190 | Phosphorylation | Uniprot | |
| S194 | Phosphorylation | Uniprot | |
| S238 | Phosphorylation | Uniprot | |
| S240 | Phosphorylation | Uniprot | |
| S241 | Phosphorylation | Uniprot | |
| T243 | Phosphorylation | Uniprot | |
| S244 | Phosphorylation | Uniprot | |
| T246 | Phosphorylation | Uniprot | |
| T251 | Phosphorylation | Uniprot | |
| S276 | Phosphorylation | Q14012 (CAMK1) | Uniprot |
| R278 | Methylation | Uniprot | |
| S284 | Phosphorylation | Uniprot | |
| K286 | Ubiquitination | Uniprot | |
| S288 | Phosphorylation | Q00535 (CDK5) | Uniprot |
| S295 | Phosphorylation | Q14012 (CAMK1) | Uniprot |
| S361 | Phosphorylation | Uniprot | |
| S425 | Phosphorylation | Uniprot | |
| T436 | Phosphorylation | Uniprot | |
| S438 | Phosphorylation | Uniprot | |
| S448 | Phosphorylation | Uniprot | |
| T561 | Phosphorylation | Uniprot | |
| T631 | Phosphorylation | Uniprot | |
| S634 | Phosphorylation | Uniprot |
研究背景
Plays a role in the process of neurogenesis. Required throughout embryonic neurogenesis to maintain neural progenitor cells, also called radial glial cells (RGCs), by allowing their daughter cells to choose progenitor over neuronal cell fate. Not required for the proliferation of neural progenitor cells before the onset of neurogenesis. Also involved postnatally in the subventricular zone (SVZ) neurogenesis by regulating SVZ neuroblasts survival and ependymal wall integrity. May also mediate local repair of brain ventricular wall damage.
Phosphorylated on Ser-276 and Ser-295 by CaMK1.
Isoform 1 and isoform 2 are ubiquitinated by LNX leading to their subsequent proteasomal degradation (By similarity). Ubiquitinated; mediated by SIAH1 and leading to its subsequent proteasomal degradation.
Membrane>Peripheral membrane protein.
Interacts with CDH1 and TFAP2B (By similarity). Interacts with EPS15, LNX and NOTCH1. May interact with DUOXA1. Interacts with RALBP1 in a complex also containing EPN1 and TFAP2A during interphase and mitosis.
研究领域
· Environmental Information Processing > Signal transduction > Notch signaling pathway. (View pathway)
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