(1)
(3)
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF7696, RRID:AB_2841167.
展开/折叠
FH; FHC; LDL R; LDL receptor; LDLCQ2; Ldlr; LDLR_HUMAN; Low Density Lipoprotein Receptor; Low density lipoprotein receptor class A domain containing protein 3; Low density lipoprotein receptor familial hypercholesterolemia; Low-density lipoprotein receptor;
抗原和靶标
- P01130 LDLR_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MGPWGWKLRWTVALLLAAAGTAVGDRCERNEFQCQDGKCISYKWVCDGSAECQDGSDESQETCLSVTCKSGDFSCGGRVNRCIPQFWRCDGQVDCDNGSDEQGCPPKTCSQDEFRCHDGKCISRQFVCDSDRDCLDGSDEASCPVLTCGPASFQCNSSTCIPQLWACDNDPDCEDGSDEWPQRCRGLYVFQGDSSPCSAFEFHCLSGECIHSSWRCDGGPDCKDKSDEENCAVATCRPDEFQCSDGNCIHGSRQCDREYDCKDMSDEVGCVNVTLCEGPNKFKCHSGECITLDKVCNMARDCRDWSDEPIKECGTNECLDNNGGCSHVCNDLKIGYECLCPDGFQLVAQRRCEDIDECQDPDTCSQLCVNLEGGYKCQCEEGFQLDPHTKACKAVGSIAYLFFTNRHEVRKMTLDRSEYTSLIPNLRNVVALDTEVASNRIYWSDLSQRMICSTQLDRAHGVSSYDTVISRDIQAPDGLAVDWIHSNIYWTDSVLGTVSVADTKGVKRKTLFRENGSKPRAIVVDPVHGFMYWTDWGTPAKIKKGGLNGVDIYSLVTENIQWPNGITLDLLSGRLYWVDSKLHSISSIDVNGGNRKTILEDEKRLAHPFSLAVFEDKVFWTDIINEAIFSANRLTGSDVNLLAENLLSPEDMVLFHNLTQPRGVNWCERTTLSNGGCQYLCLPAPQINPHSPKFTCACPDGMLLARDMRSCLTEAEAAVATQETSTVRLKVSSTAVRTQHTTTRPVPDTSRLPGATPGLTTVEIVTMSHQALGDVAGRGNEKKPSSVRALSIVLPIVLLVFLCLGVFLLWKNWRLKNINSINFDNPVYQKTTEDEVHICHNQDGYSYPSRQMVSLEDDVA
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P01130 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K38 | Ubiquitination | Uniprot | |
| S110 | Phosphorylation | Uniprot | |
| N156 | N-Glycosylation | Uniprot | |
| N272 | N-Glycosylation | Uniprot | |
| K390 | Ubiquitination | Uniprot | |
| Y465 | Phosphorylation | Uniprot | |
| S470 | Phosphorylation | Uniprot | |
| S517 | Phosphorylation | Uniprot | |
| K596 | Ubiquitination | Uniprot | |
| K603 | Ubiquitination | Uniprot | |
| N657 | N-Glycosylation | Uniprot | |
| T713 | Phosphorylation | Uniprot | |
| T721 | O-Glycosylation | Uniprot | |
| T724 | O-Glycosylation | Uniprot | |
| S725 | O-Glycosylation | Uniprot | |
| T726 | O-Glycosylation | Uniprot | |
| K730 | Ubiquitination | Uniprot | |
| S732 | O-Glycosylation | Uniprot | |
| S733 | O-Glycosylation | Uniprot | |
| T734 | O-Glycosylation | Uniprot | |
| T738 | O-Glycosylation | Uniprot | |
| T741 | O-Glycosylation | Uniprot | |
| T742 | O-Glycosylation | Uniprot | |
| T743 | O-Glycosylation | Uniprot | |
| S750 | O-Glycosylation | Uniprot | |
| S820 | Phosphorylation | Uniprot | |
| Y828 | Phosphorylation | Uniprot | |
| K830 | Ubiquitination | Uniprot | |
| Y845 | Phosphorylation | Uniprot | |
| S846 | Phosphorylation | Uniprot | |
| Y847 | Phosphorylation | Uniprot |
研究背景
Binds LDL, the major cholesterol-carrying lipoprotein of plasma, and transports it into cells by endocytosis. In order to be internalized, the receptor-ligand complexes must first cluster into clathrin-coated pits.
(Microbial infection) Acts as a receptor for hepatitis C virus in hepatocytes, but not through a direct interaction with viral proteins.
(Microbial infection) Acts as a receptor for Vesicular stomatitis virus.
(Microbial infection) In case of HIV-1 infection, may function as a receptor for extracellular Tat in neurons, mediating its internalization in uninfected cells.
N- and O-glycosylated.
Ubiquitinated by MYLIP leading to degradation.
Cell membrane>Single-pass type I membrane protein. Membrane>Clathrin-coated pit. Golgi apparatus. Early endosome. Late endosome. Lysosome.
Note: Rapidly endocytosed upon ligand binding.
Interacts (via NPXY motif) with DAB2 (via PID domain); the interaction is impaired by tyrosine phosphorylation of the NPXY motif (By similarity). Interacts (via NPXY motif) with LDLRAP1 (via PID domain). Interacts with ARRB1. Interacts with SNX17. Interacts with the full-length immature form of PCSK9 (via C-terminus).
(Microbial infection) Interacts with vesicular stomatitis virus glycoprotein.
(Microbial infection) May interact with HIV-1 Tat.
The NPXY motif mediates the interaction with the clathrin adapter DAB2 and with LDLRAP1 which are involved in receptor internalization. A few residues outside the motif also play a role in the interaction.
Belongs to the LDLR family.
研究领域
· Cellular Processes > Transport and catabolism > Endocytosis. (View pathway)
· Human Diseases > Infectious diseases: Parasitic > Toxoplasmosis.
· Human Diseases > Infectious diseases: Viral > Hepatitis C.
· Organismal Systems > Endocrine system > Ovarian steroidogenesis.
· Organismal Systems > Endocrine system > Aldosterone synthesis and secretion.
· Organismal Systems > Digestive system > Cholesterol metabolism.
文献引用
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