SPEE Antibody - #DF7732

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产品: SPEE 抗体
货号: DF7732
描述: Rabbit polyclonal antibody to SPEE
应用: WB IHC
反应: Human, Mouse, Rat
预测: Pig, Zebrafish, Bovine, Horse, Sheep, Dog, Chicken, Xenopus
分子量: 33 kDa; 34kD(Calculated).
蛋白号: P19623
RRID: AB_2841200

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

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相关下载
MS Report
HPLC Report
MSDS
说明书
COA
实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
IHC 1:50-1:200, WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(100%), Zebrafish(90%), Bovine(100%), Horse(100%), Sheep(100%), Dog(100%), Chicken(100%), Xenopus(90%)
克隆:
Polyclonal
特异性:
SPEE Antibody detects endogenous levels of total SPEE.
RRID:
AB_2841200
引用格式: Affinity Biosciences Cat# DF7732, RRID:AB_2841200.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

OTTHUMP00000002170; PAPT; Putrescine aminopropyltransferase; SPDSY; SPEE_HUMAN; Spermidine synthase 1; Spermidine synthase; SPS1; Srm; SRML1;

抗原和靶标

免疫原:
Uniprot:

P19623(SPEE_HUMAN) >>Visit HPA database.

基因/基因ID:
SRM(6723)
序列:
MEPGPDGPAASGPAAIREGWFRETCSLWPGQALSLQVEQLLHHRRSRYQDILVFRSKTYGNVLVLDGVIQCTERDEFSYQEMIANLPLCSHPNPRKVLIIGGGDGGVLREVVKHPSVESVVQCEIDEDVIQVSKKFLPGMAIGYSSSKLTLHVGDGFEFMKQNQDAFDVIITDSSDPMGPAESLFKESYYQLMKTALKEDGVLCCQGECQWLHLDLIKEMRQFCQSLFPVVAYAYCTIPTYPSGQIGFMLCSKNPSTNFQEPVQPLTQQQVAQMQLKYYNSDVHRAAFVLPEFARKALNDVS

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Chicken
100
Xenopus
90
Zebrafish
90
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P19623 作为底物

Site PTM Type Enzyme
M1 Acetylation
R17 Methylation
R22 Methylation
Y48 Phosphorylation
C71 S-Nitrosylation
T72 Phosphorylation
K96 Ubiquitination
K113 Ubiquitination
S116 Phosphorylation
K134 Ubiquitination
K135 Ubiquitination
S147 Phosphorylation
K194 Ubiquitination
K198 Ubiquitination
K253 Sumoylation
K253 Ubiquitination
K277 Sumoylation
K277 Ubiquitination
K296 Sumoylation
K296 Ubiquitination

研究背景

功能:

Catalyzes the production of spermidine from putrescine and decarboxylated S-adenosylmethionine (dcSAM). Has a strong preference for putrescine as substrate, and has very low activity towards 1,3-diaminopropane. Has extremely low activity towards spermidine.

亚基结构:

Homodimer or homotetramer.

蛋白家族:

Belongs to the spermidine/spermine synthase family.

研究领域

· Metabolism > Amino acid metabolism > Cysteine and methionine metabolism.

· Metabolism > Amino acid metabolism > Arginine and proline metabolism.

· Metabolism > Metabolism of other amino acids > beta-Alanine metabolism.

· Metabolism > Metabolism of other amino acids > Glutathione metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

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