VAMP8 Antibody - #DF7818

(1)   (2)  
产品: VAMP8 抗体
货号: DF7818
描述: Rabbit polyclonal antibody to VAMP8
应用: WB
反应: Human
预测: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
分子量: 15 kDa; 11kD(Calculated).
蛋白号: Q9BV40
RRID: AB_2841267

浏览相似产品>>

   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

联系销售
相关下载
MS Report
HPLC Report
MSDS
说明书
COA
实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human
预测:
Pig(100%), Zebrafish(83%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Xenopus(92%)
克隆:
Polyclonal
特异性:
VAMP8 Antibody detects endogenous levels of total VAMP8.
RRID:
AB_2841267
引用格式: Affinity Biosciences Cat# DF7818, RRID:AB_2841267.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

EDB; Endobrevin; VAMP 8; VAMP-8; VAMP8; VAMP8_HUMAN; Vesicle associated membrane protein 8; Vesicle-associated membrane protein 8;

抗原和靶标

免疫原:
Uniprot:

Q9BV40(VAMP8_HUMAN) >>Visit HPA database.

基因/基因ID:
VAMP8(8673)
表达:
Q9BV40 VAMP8_HUMAN:

Platelets.

序列:
MEEASEGGGNDRVRNLQSEVEGVKNIMTQNVERILARGENLEHLRNKTEDLEATSEHFKTTSQKVARKFWWKNVKMIVLICVIVFIIILFIVLFATGAFS

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Xenopus
92
Zebrafish
83
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - Q9BV40 作为底物

Site PTM Type Enzyme
M1 Acetylation
S5 Phosphorylation
R12 Methylation
S18 Phosphorylation
K24 Ubiquitination
T28 Phosphorylation
K47 Ubiquitination
T48 Phosphorylation
T54 Phosphorylation
S55 Phosphorylation
K59 Ubiquitination
K64 Ubiquitination

研究背景

功能:

SNAREs, soluble N-ethylmaleimide-sensitive factor-attachment protein receptors, are essential proteins for fusion of cellular membranes. SNAREs localized on opposing membranes assemble to form a trans-SNARE complex, an extended, parallel four alpha-helical bundle that drives membrane fusion. VAMP8 is a SNARE involved in autophagy through the direct control of autophagosome membrane fusion with the lysososome membrane via its interaction with the STX17-SNAP29 binary t-SNARE complex. Also required for dense-granule secretion in platelets. Plays also a role in regulated enzyme secretion in pancreatic acinar cells (By similarity). Involved in the abscission of the midbody during cell division, which leads to completely separate daughter cells (By similarity). Involved in the homotypic fusion of early and late endosomes (By similarity).

细胞定位:

Lysosome membrane>Single-pass type IV membrane protein. Early endosome membrane>Single-pass type IV membrane protein. Late endosome membrane>Single-pass type IV membrane protein. Cell membrane>Single-pass type IV membrane protein.
Note: Perinuclear vesicular structures of the early and late endosomes, coated pits, and trans-Golgi (By similarity). Sub-tight junctional domain in retinal pigment epithelium cells. Midbody region during cytokinesis. Lumenal oriented, apical membranes of nephric tubular cell (By similarity). Cycles through the apical but not through the basolateral plasma membrane (By similarity). Apical region of acinar cells; in zymogen granule membranes (By similarity).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Platelets.

亚基结构:

Forms a SNARE complex composed of VAMP8, SNAP29 and STX17 involved in fusion of autophagosome with lysosome. Found in a number of SNARE complexes with NAPA, SNAP23, SNAP25, STX1A, STX4, STX7, STX8 and VTI1B. Interacts with PICALM (By similarity). SNARE complex formation and binding by PICALM are mutually exclusive processes for VAMP8 (By similarity). Interacts with SBF2/MTMR13. Interacts with RAB21 (in GTP-bound form) in response to starvation; the interaction probably regulates VAMP8 endolysosomal trafficking.

蛋白家族:

Belongs to the synaptobrevin family.

研究领域

· Cellular Processes > Transport and catabolism > Autophagy - animal.   (View pathway)

· Genetic Information Processing > Folding, sorting and degradation > SNARE interactions in vesicular transport.

· Organismal Systems > Immune system > Platelet activation.   (View pathway)

文献引用

1). mTOR deletion ameliorates CD4+ T cell apoptosis during sepsis by improving autophagosome-lysosome fusion. APOPTOSIS (PubMed: 35435531) [IF=7.2]

Application: WB    Species: Mice    Sample: CD4 + T cell

Fig. 3 Transcription factor EB (TFEB) phosphorylation and SNARE expression in septic mice. (A, a–e) Protein levels of phospho (p)-TFEB, syntaxin 17 (STX17), vesicle associated membrane protein 8 (VAMP8), and synaptosomal-associated protein 29 (SNAP29) in CD4 + T cells were quantified by western blotting in wild-type (WT) sham-treated mice, WT CLP mice, mammalian target of rapamycin (mTOR) knockout via Cre expression under the control of lymphocyte-specific protein tyrosine kinase (Lck) (Lck-mTOR) CLP mice, and tuberous sclerosis complex 1 (TSC1) knockout (Lck-TSC1) CLP mice. The amount of each protein level was normalized to that of β-actin. Means ± standard deviations (SDs) of four mice per group are shown. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001

Application: WB    Species: mouse    Sample: CD4+T cells

Fig. 3| Transcription factor EB (TFEB) phosphorylation and SNARE expression in septic mice. (A, a–e) Protein levels of phospho (p)-TFEB, syntaxin 17 (STX17), vesicle associated membrane protein 8 (VAMP8), and synaptosomal-associated protein 29 (SNAP29) in CD4+T cells were quantifed by western blotting in wild-type (WT)sham-treated mice, WT CLP mice, mammalian target of rapamycin(mTOR) knockout via Cre expression under the control of lymphocyte-specifc protein tyrosine kinase (Lck) (Lck-mTOR) CLP mice,and tuberous sclerosis complex 1 (TSC1) knockout (Lck-TSC1) CLP mice.

限制条款

产品的规格、报价、验证数据请以官网为准，官网链接：www.affbiotech.com | www.affbiotech.cn（简体中文）| www.affbiotech.jp（日本語）

产品的数据信息为Affinity所有，未经授权不得收集Affinity官网数据或资料用于商业用途，对抄袭产品数据的行为我们将保留诉诸法律的权利。

产品相关数据会因产品批次、产品检测情况随时调整，如您已订购该产品，请以订购时随货说明书为准，否则请以官网内容为准，官网内容有改动时恕不另行通知。

Affinity保证所销售产品均经过严格质量检测。如您购买的商品在规定时间内出现问题需要售后时，请您在Affinity官方渠道提交售后申请。

产品仅供科学研究使用。不用于诊断和治疗。 

产品未经授权不得转售。

Affinity Biosciences将不会对在使用我们的产品时可能发生的专利侵权或其他侵权行为负责。Affinity Biosciences, Affinity Biosciences标志和所有其他商标所有权归Affinity Biosciences LTD.