RASSF2 Antibody - #DF8437

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产品: RASSF2 抗体
货号: DF8437
描述: Rabbit polyclonal antibody to RASSF2
应用: WB IHC IF/ICC
反应: Human, Mouse, Rat
预测: Pig, Horse, Sheep, Rabbit, Dog, Xenopus
分子量: 37 kDa; 38kD(Calculated).
蛋白号: P50749
RRID: AB_2841683

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   规格 价格 库存
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

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MSDS
说明书
COA
实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:1000-3000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Xenopus(100%)
克隆:
Polyclonal
特异性:
RASSF2 Antibody detects endogenous levels of total RASSF2.
RRID:
AB_2841683
引用格式: Affinity Biosciences Cat# DF8437, RRID:AB_2841683.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

CENP 34; Centromere protein 34; Ras association (RalGDS/AF 6) domain family 2; Ras association (RalGDS/AF-6) domain family member 2; Ras association domain containing protein 2; Ras association domain-containing protein 2; RASF2_HUMAN; RASFADIN; RASSF2; RP4-599I11.1;

抗原和靶标

免疫原:
Uniprot:

P50749(RASF2_HUMAN) >>Visit HPA database.

基因/基因ID:
RASSF2(9770)
表达:
P50749 RASF2_HUMAN:

Widely expressed with highest levels in brain, placenta, peripheral blood and lung. Frequently down-regulated in lung tumor cell lines.

序列:
MDYSHQTSLVPCGQDKYISKNELLLHLKTYNLYYEGQNLQLRHREEEDEFIVEGLLNISWGLRRPIRLQMQDDNERIRPPPSSSSWHSGCNLGAQGTTLKPLTVPKVQISEVDAPPEGDQMPSSTDSRGLKPLQEDTPQLMRTRSDVGVRRRGNVRTPSDQRRIRRHRFSINGHFYNHKTSVFTPAYGSVTNVRINSTMTTPQVLKLLLNKFKIENSAEEFALYVVHTSGEKQKLKATDYPLIARILQGPCEQISKVFLMEKDQVEEVTYDVAQYIKFEMPVLKSFIQKLQEEEDREVKKLMRKYTVLRLMIRQRLEEIAETPATI

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Sheep
100
Dog
100
Xenopus
100
Rabbit
100
Bovine
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P50749 作为底物

Site PTM Type Enzyme
S8 Phosphorylation
K20 Ubiquitination
K100 Ubiquitination
T137 Phosphorylation
R142 Methylation
T143 Phosphorylation
R144 Methylation
S145 Phosphorylation
R151 Methylation
R165 Methylation
S170 Phosphorylation
K206 Ubiquitination
K211 Ubiquitination
Y224 Phosphorylation
Y270 Phosphorylation
K289 Ubiquitination
Y305 Phosphorylation
T306 Phosphorylation
T325 Phosphorylation

研究背景

功能:

Potential tumor suppressor. Acts as a KRAS-specific effector protein. May promote apoptosis and cell cycle arrest. Stabilizes STK3/MST2 by protecting it from proteasomal degradation.

翻译修饰:

Phosphorylated by STK3/MST2 and STK4/MST1.

细胞定位:

Nucleus. Cytoplasm. Chromosome>Centromere>Kinetochore.
Note: Translocates to the cytoplasm in the presence of STK3/MST2 AND STK4/MST1.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Widely expressed with highest levels in brain, placenta, peripheral blood and lung. Frequently down-regulated in lung tumor cell lines.

亚基结构:

Interacts directly with activated KRAS in a GTP-dependent manner. Interacts (via SARAH domain) with STK3/MST2 AND STK4/MST1.

研究领域

· Environmental Information Processing > Signal transduction > Hippo signaling pathway - multiple species.   (View pathway)

文献引用

1). MiR-93-5p promotes gastric cancer-cell progression via inactivation of the Hippo signaling pathway. GENE (PubMed: 29045821) [IF=3.5]

Application: WB    Species: human    Sample:

Figure 4. FAT4 and LATS2 are direct miR-93-5p targets. (A) Quantitative PCR results showed that miR-93-5p overexpression suppressed FAT4 and LATS2 mRNA expression in SGC-7901 cells, while conversely, the opposite result was observed in HGC-27 cells exhibiting downregulated miR-93-5p expression. RUNX3 and RASSF2 mRNA levels were unchanged in response to either up- or downregulation of miR-93-5p. (B) Western blot analyses of FAT4, LATS2, RUNX3, and RASSF2 expression after transfection with miR-93-5p mimics and/or the miR-93-5p inhibitor.

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