DIO3 Antibody - #DF8532

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产品: DIO3 抗体
货号: DF8532
描述: Rabbit polyclonal antibody to DIO3
应用: WB IHC
反应: Human, Mouse, Rat, Monkey
预测: Pig, Bovine, Sheep, Dog
分子量: 31 kDa; 34kD(Calculated).
蛋白号: P55073
RRID: AB_2841736

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

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说明书
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实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat,Monkey
预测:
Pig(100%), Bovine(100%), Sheep(100%), Dog(100%)
克隆:
Polyclonal
特异性:
DIO3 Antibody detects endogenous levels of total DIO3.
RRID:
AB_2841736
引用格式: Affinity Biosciences Cat# DF8532, RRID:AB_2841736.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

5DIII; D3; Deiodinase iodothyronine type 3; Deiodinase iodothyronine type III; Dio 3; Dio3; DIOIII; IOD3_HUMAN; Iodothyronine deiodinase placental type; MGC124117; MGC124118; Thyroxine deiodinase type III; Thyroxine deiodinase type III (selenoprotein); TXDI3; Type 3 DI; Type 3 iodothyronine selenodeiodinase; Type III 5' deiodinase; Type III iodothyronine deiodinase; Type-III 5''-deiodinase;

抗原和靶标

免疫原:

A synthesized peptide derived from human DIO3, corresponding to a region within the internal amino acids.

Uniprot:

P55073(IOD3_HUMAN) >>Visit HPA database.

基因/基因ID:
DIO3(1735)
表达:
P55073 IOD3_HUMAN:

Expressed in placenta and several fetal tissues.

序列:
MPRQATSRLVVGEGEGSQGASGPAATMLRSLLLHSLRLCAQTASCLVLFPRFLGTAFMLWLLDFLCIRKHFLGRRRRGQPEPEVELNSEGEEVPPDDPPICVSDDNRLCTLASLKAVWHGQKLDFFKQAHEGGPAPNSEVVLPDGFQSQHILDYAQGNRPLVLNFGSCTUPPFMARMSAFQRLVTKYQRDVDFLIIYIEEAHPSDGWVTTDSPYIIPQHRSLEDRVSAARVLQQGAPGCALVLDTMANSSSSAYGAYFERLYVIQSGTIMYQGGRGPDGYQVSELRTWLERYDEQLHGARPRRV

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
100
Bovine
100
Sheep
100
Dog
100
Chicken
75
Xenopus
67
Horse
0
Zebrafish
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P55073 作为底物

Site PTM Type Enzyme
S252 Phosphorylation

研究背景

功能:

Responsible for the deiodination of T4 (3,5,3',5'-tetraiodothyronine) into RT3 (3,3',5'-triiodothyronine) and of T3 (3,5,3'-triiodothyronine) into T2 (3,3'-diiodothyronine). RT3 and T2 are inactive metabolites. May play a role in preventing premature exposure of developing fetal tissues to adult levels of thyroid hormones. Can regulate circulating fetal thyroid hormone concentrations throughout gestation. Essential role for regulation of thyroid hormone inactivation during embryological development.

细胞定位:

Cell membrane>Single-pass type II membrane protein. Endosome membrane>Single-pass type II membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Expressed in placenta and several fetal tissues.

亚基结构:

Homodimer. May undergo minor heretodimerization with DIO1 and DIO2.

蛋白家族:

Belongs to the iodothyronine deiodinase family.

研究领域

· Organismal Systems > Endocrine system > Thyroid hormone signaling pathway.   (View pathway)

文献引用

1). Dlk1-Dio3 Locus-Derived lncRNA Dio3os Affects Growth, Development and Reproductive Activity in Mice. , 2023

Application: WB    Species: Mouse    Sample: ESCs

Fig. 2: (A) Effects of Dio3os on Dlk1, Gtl2 and Dio3 in mouse ESCs. (B) Effects of Dio3os on DIO3 protein in mouse ESCs. (C) Relative mRNA expression levels of Dio3os and Dio3 in different mouse tissues.

Application: WB    Species: Mouse    Sample: ESCs

Fig. 2: (A) Effects of Dio3os on Dlk1, Gtl2 and Dio3 in mouse ESCs. (B) Effects of Dio3os on DIO3 protein in mouse ESCs. (C) Relative mRNA expression levels of Dio3os and Dio3 in different mouse tissues.

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