(1)
(5)
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF8542, RRID:AB_2841746.
展开/折叠
Equilibrative NBMPR-sensitive nucleoside transporter; equilibrative nitrobenzylmercaptopurine riboside (NBMPR)-sensitive nucleoside transporter; Equilibrative nitrobenzylmercaptopurine riboside-sensitive nucleoside transporter; Equilibrative nucleoside transporter 1; es-type; MGC1465; MGC3778; Nucleoside transporter; Nucleoside transporter, es-type; OTTHUMP00000016506; OTTHUMP00000016507; OTTHUMP00000016508; OTTHUMP00000016509; OTTHUMP00000016510; OTTHUMP00000016511; OTTHUMP00000016512; S29A1_HUMAN; Slc29a1; solute carrier family 29 (equilibrative nucleoside transporter), member 1; solute carrier family 29 (nucleoside transporters), member 1; Solute carrier family 29 member 1;
抗原和靶标
Detected in erythrocytes (at protein level). Expressed in heart, brain, mammary gland, erythrocytes and placenta, and also in fetal liver and spleen.
- Q99808 S29A1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MTTSHQPQDRYKAVWLIFFMLGLGTLLPWNFFMTATQYFTNRLDMSQNVSLVTAELSKDAQASAAPAAPLPERNSLSAIFNNVMTLCAMLPLLLFTYLNSFLHQRIPQSVRILGSLVAILLVFLITAILVKVQLDALPFFVITMIKIVLINSFGAILQGSLFGLAGLLPASYTAPIMSGQGLAGFFASVAMICAIASGSELSESAFGYFITACAVIILTIICYLGLPRLEFYRYYQQLKLEGPGEQETKLDLISKGEEPRAGKEESGVSVSNSQPTNESHSIKAILKNISVLAFSVCFIFTITIGMFPAVTVEVKSSIAGSSTWERYFIPVSCFLTFNIFDWLGRSLTAVFMWPGKDSRWLPSLVLARLVFVPLLLLCNIKPRRYLTVVFEHDAWFIFFMAAFAFSNGYLASLCMCFGPKKVKPAEAETAGAIMAFFLCLGLALGAVFSFLFRAIV
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q99808 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| N48 | N-Glycosylation | Uniprot | |
| S63 | O-Glycosylation | Uniprot | |
| S63 | Phosphorylation | Uniprot | |
| T143 | Phosphorylation | Uniprot | |
| K239 | Ubiquitination | Uniprot | |
| T248 | Phosphorylation | Uniprot | |
| K249 | Ubiquitination | Uniprot | |
| S254 | Phosphorylation | Uniprot | |
| K255 | Ubiquitination | Uniprot | |
| K263 | Ubiquitination | Uniprot | |
| S266 | Phosphorylation | Uniprot | |
| S269 | Phosphorylation | Uniprot | |
| S271 | Phosphorylation | Uniprot | |
| S273 | Phosphorylation | Uniprot | |
| S281 | Phosphorylation | Q05655 (PRKCD) | Uniprot |
| K283 | Ubiquitination | Uniprot | |
| K287 | Ubiquitination | Uniprot | |
| K356 | Ubiquitination | Uniprot | |
| K381 | Ubiquitination | Uniprot |
研究背景
Mediates both influx and efflux of nucleosides across the membrane (equilibrative transporter). It is sensitive (ES) to low concentrations of the inhibitor nitrobenzylmercaptopurine riboside (NBMPR) and is sodium-independent. It has a higher affinity for adenosine. Inhibited by dipyridamole and dilazep (anticancer chemotherapeutics drugs).
Glycosylated.
Basolateral cell membrane>Multi-pass membrane protein. Apical cell membrane>Multi-pass membrane protein. Cell membrane>Multi-pass membrane protein.
Note: Predominantly localized in the basolateral membrane in polarized MDCK cells.
Detected in erythrocytes (at protein level). Expressed in heart, brain, mammary gland, erythrocytes and placenta, and also in fetal liver and spleen.
Identified in a complex with STOM.
Belongs to the SLC29A/ENT transporter (TC 2.A.57) family.
研究领域
· Human Diseases > Substance dependence > Alcoholism.
文献引用
Application: WB Species: mouse Sample: hippocampus
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