(5)
(12)
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF3027, RRID:AB_2834458.
展开/折叠
BHLHE32; bHLHe32; Class E basic helix-loop-helix protein 32; CMM8; Homolog of mouse microphthalmia; Mi; Microphthalmia associated transcription factor; Microphthalmia, mouse, homolog of; Microphthalmia-associated transcription factor; MITF; MITF_HUMAN; mitfa; nacre; WS2; WS2A; z3A.1;
抗原和靶标
Isoform M is exclusively expressed in melanocytes and melanoma cells. Isoform A and isoform H are widely expressed in many cell types including melanocytes and retinal pigment epithelium (RPE). Isoform C is expressed in many cell types including RPE but not in melanocyte-lineage cells. Isoform Mdel is widely expressed in melanocytes, melanoma cell lines and tissues, but almost undetectable in non-melanoma cell lines.
- O75030 MITF_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MQSESGIVPDFEVGEEFHEEPKTYYELKSQPLKSSSSAEHPGASKPPISSSSMTSRILLRQQLMREQMQEQERREQQQKLQAAQFMQQRVPVSQTPAINVSVPTTLPSATQVPMEVLKVQTHLENPTKYHIQQAQRQQVKQYLSTTLANKHANQVLSLPCPNQPGDHVMPPVPGSSAPNSPMAMLTLNSNCEKEGFYKFEEQNRAESECPGMNTHSRASCMQMDDVIDDIISLESSYNEEILGLMDPALQMANTLPVSGNLIDLYGNQGLPPPGLTISNSCPANLPNIKRELTACIFPTESEARALAKERQKKDNHNLIERRRRFNINDRIKELGTLIPKSNDPDMRWNKGTILKASVDYIRKLQREQQRAKELENRQKKLEHANRHLLLRIQELEMQARAHGLSLIPSTGLCSPDLVNRIIKQEPVLENCSQDLLQHHADLTCTTTLDLTDGTITFNNNLGTGTEANQAYSVPTKMGSKLEDILMDDTLSPVGVTDPLLSSVSPGASKTSSRRSSMSMEETEHTC
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - O75030 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| T54 | Phosphorylation | Uniprot | |
| S55 | Phosphorylation | Uniprot | |
| K79 | Sumoylation | Uniprot | |
| K128 | Ubiquitination | Uniprot | |
| K140 | Sumoylation | Uniprot | |
| S157 | Phosphorylation | Uniprot | |
| S180 | Phosphorylation | P28482 (MAPK1) | Uniprot |
| K198 | Ubiquitination | Uniprot | |
| S216 | Phosphorylation | Uniprot | |
| K289 | Sumoylation | Uniprot | |
| S301 | Phosphorylation | Uniprot | |
| K308 | Ubiquitination | Uniprot | |
| R330 | Methylation | Uniprot | |
| K350 | Ubiquitination | Uniprot | |
| S357 | Phosphorylation | Uniprot | |
| Y360 | Phosphorylation | Uniprot | |
| K372 | Ubiquitination | Uniprot | |
| S405 | Phosphorylation | P49840 (GSK3A) | Uniprot |
| S409 | Phosphorylation | Q15418 (RPS6KA1) | Uniprot |
| S414 | Phosphorylation | Q16539 (MAPK14) | Uniprot |
| K423 | Sumoylation | Uniprot | |
| T489 | Phosphorylation | Uniprot | |
| S491 | Phosphorylation | Uniprot | |
| S502 | Phosphorylation | Uniprot | |
| S504 | Phosphorylation | Uniprot | |
| S508 | Phosphorylation | Uniprot | |
| T510 | Phosphorylation | Uniprot | |
| S511 | Phosphorylation | Uniprot | |
| S512 | Phosphorylation | Uniprot | |
| S515 | Phosphorylation | Uniprot | |
| S516 | Phosphorylation | Uniprot |
研究背景
Transcription factor that regulates the expression of genes with essential roles in cell differentiation, proliferation and survival. Binds to M-boxes (5'-TCATGTG-3') and symmetrical DNA sequences (E-boxes) (5'-CACGTG-3') found in the promoters of target genes, such as BCL2 and tyrosinase (TYR). Plays an important role in melanocyte development by regulating the expression of tyrosinase (TYR) and tyrosinase-related protein 1 (TYRP1). Plays a critical role in the differentiation of various cell types, such as neural crest-derived melanocytes, mast cells, osteoclasts and optic cup-derived retinal pigment epithelium.
Phosphorylation at Ser-405 significantly enhances the ability to bind the tyrosinase promoter. Phosphorylated at Ser-180 and Ser-516 following KIT signaling, trigerring a short live activation: Phosphorylation at Ser-180 and Ser-516 by MAPK and RPS6KA1, respectively, activate the transcription factor activity but also promote ubiquitination and subsequent degradation by the proteasome.
Ubiquitinated following phosphorylation at Ser-180, leading to subsequent degradation by the proteasome. Deubiquitinated by USP13, preventing its degradation.
Nucleus.
Isoform M is exclusively expressed in melanocytes and melanoma cells. Isoform A and isoform H are widely expressed in many cell types including melanocytes and retinal pigment epithelium (RPE). Isoform C is expressed in many cell types including RPE but not in melanocyte-lineage cells. Isoform Mdel is widely expressed in melanocytes, melanoma cell lines and tissues, but almost undetectable in non-melanoma cell lines.
Homodimer or heterodimer; dimerization is mediated via the coiled coil region. Efficient DNA binding requires dimerization with another bHLH protein. Binds DNA in the form of homodimer or heterodimer with either TFE3, TFEB or TFEC. Interacts with KARS1. Identified in a complex with HINT1 and CTNNB1. Interacts with VSX2 (By similarity).
The leucine zipper region is part of a larger coiled coil.
Belongs to the MiT/TFE family.
研究领域
· Human Diseases > Cancers: Overview > Pathways in cancer. (View pathway)
· Human Diseases > Cancers: Overview > Transcriptional misregulation in cancer.
· Human Diseases > Cancers: Specific types > Melanoma. (View pathway)
· Organismal Systems > Development > Osteoclast differentiation. (View pathway)
· Organismal Systems > Endocrine system > Melanogenesis.
文献引用
Application: WB Species: human Sample: HEMs
Application: WB Species: clams Sample: haemocytes
Application: WB Species: zebrafish Sample:
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