(3)
(9)
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF8624, RRID:AB_2841828.
展开/折叠
BCYM3; CMT2C; HMSN2C; osm 9 like TRP channel 4; Osm-9-like TRP channel 4; OSM9 like transient receptor potential channel 4; Osmosensitive transient receptor potential channel 4; OTRPC 4; OTRPC4; SMAL; SPSMA; SSQTL1; Transient receptor potential cation channel subfamily V member 4; Transient receptor potential protein 12; TRP 12; TRP12; TRPV 4; TrpV4; TRPV4_HUMAN; Vanilloid receptor like channel 2; Vanilloid receptor like protein 2; Vanilloid receptor related osmotically activated channel; Vanilloid receptor-like channel 2; Vanilloid receptor-like protein 2; Vanilloid receptor-related osmotically-activated channel; VR 4; VR OAC; VR-OAC; VR4; VRL 2; VRL-2; VRL2; VROAC;
抗原和靶标
Found in the synoviocytes from patients with (RA) and without (CTR) rheumatoid arthritis (at protein level).
- Q9HBA0 TRPV4_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MADSSEGPRAGPGEVAELPGDESGTPGGEAFPLSSLANLFEGEDGSLSPSPADASRPAGPGDGRPNLRMKFQGAFRKGVPNPIDLLESTLYESSVVPGPKKAPMDSLFDYGTYRHHSSDNKRWRKKIIEKQPQSPKAPAPQPPPILKVFNRPILFDIVSRGSTADLDGLLPFLLTHKKRLTDEEFREPSTGKTCLPKALLNLSNGRNDTIPVLLDIAERTGNMREFINSPFRDIYYRGQTALHIAIERRCKHYVELLVAQGADVHAQARGRFFQPKDEGGYFYFGELPLSLAACTNQPHIVNYLTENPHKKADMRRQDSRGNTVLHALVAIADNTRENTKFVTKMYDLLLLKCARLFPDSNLEAVLNNDGLSPLMMAAKTGKIGIFQHIIRREVTDEDTRHLSRKFKDWAYGPVYSSLYDLSSLDTCGEEASVLEILVYNSKIENRHEMLAVEPINELLRDKWRKFGAVSFYINVVSYLCAMVIFTLTAYYQPLEGTPPYPYRTTVDYLRLAGEVITLFTGVLFFFTNIKDLFMKKCPGVNSLFIDGSFQLLYFIYSVLVIVSAALYLAGIEAYLAVMVFALVLGWMNALYFTRGLKLTGTYSIMIQKILFKDLFRFLLVYLLFMIGYASALVSLLNPCANMKVCNEDQTNCTVPTYPSCRDSETFSTFLLDLFKLTIGMGDLEMLSSTKYPVVFIILLVTYIILTFVLLLNMLIALMGETVGQVSKESKHIWKLQWATTILDIERSFPVFLRKAFRSGEMVTVGKSSDGTPDRRWCFRVDEVNWSHWNQNLGIINEDPGKNETYQYYGFSHTVGRLRRDRWSSVVPRVVELNKNSNPDEVVVPLDSMGNPRCDGHQQGYPRKWRTDDAPL
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q9HBA0 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| Y91 | Phosphorylation | Uniprot | |
| Y110 | Phosphorylation | Uniprot | |
| T112 | Phosphorylation | Uniprot | |
| Y113 | Phosphorylation | Uniprot | |
| S134 | Phosphorylation | Uniprot | |
| S162 | Phosphorylation | Q05655 (PRKCD) | Uniprot |
| T175 | Phosphorylation | Q05655 (PRKCD) | Uniprot |
| S189 | Phosphorylation | Q05655 (PRKCD) | Uniprot |
| Y253 | Phosphorylation | P07948 (LYN) | Uniprot |
| S319 | Phosphorylation | Uniprot | |
| T335 | Phosphorylation | Uniprot | |
| K344 | Ubiquitination | Uniprot | |
| Y411 | Phosphorylation | Uniprot | |
| S470 | Phosphorylation | Uniprot | |
| T505 | Phosphorylation | Uniprot | |
| Y508 | Phosphorylation | Uniprot | |
| Y805 | Phosphorylation | Uniprot | |
| S824 | Phosphorylation | P17612 (PRKACA) | Uniprot |
研究背景
Non-selective calcium permeant cation channel involved in osmotic sensitivity and mechanosensitivity. Activation by exposure to hypotonicity within the physiological range exhibits an outward rectification. Also activated by heat, low pH, citrate and phorbol esters. Increase of intracellular Ca(2+) potentiates currents. Channel activity seems to be regulated by a calmodulin-dependent mechanism with a negative feedback mechanism. Promotes cell-cell junction formation in skin keratinocytes and plays an important role in the formation and/or maintenance of functional intercellular barriers (By similarity). Acts as a regulator of intracellular Ca(2+) in synoviocytes. Plays an obligatory role as a molecular component in the nonselective cation channel activation induced by 4-alpha-phorbol 12,13-didecanoate and hypotonic stimulation in synoviocytes and also regulates production of IL-8. Together with PKD2, forms mechano- and thermosensitive channels in cilium. Negatively regulates expression of PPARGC1A, UCP1, oxidative metabolism and respiration in adipocytes (By similarity). Regulates expression of chemokines and cytokines related to proinflammatory pathway in adipocytes (By similarity). Together with AQP5, controls regulatory volume decrease in salivary epithelial cells (By similarity). Required for normal development and maintenance of bone and cartilage.
Non-selective calcium permeant cation channel involved in osmotic sensitivity and mechanosensitivity. Activation by exposure to hypotonicity within the physiological range exhibits an outward rectification. Also activated by phorbol esters. Has the same channel activity as isoform 1, and is activated by the same stimuli.
Lacks channel activity, due to impaired oligomerization and intracellular retention.
Lacks channel activity, due to impaired oligomerization and intracellular retention.
Lacks channel activity, due to impaired oligomerization and intracellular retention.
N-glycosylated.
Apical cell membrane>Multi-pass membrane protein. Cell junction>Adherens junction. Cell projection>Cilium.
Note: Assembly of the putative homotetramer occurs primarily in the endoplasmic reticulum.
Cell membrane.
Cell membrane.
Endoplasmic reticulum.
Endoplasmic reticulum.
Endoplasmic reticulum.
Found in the synoviocytes from patients with (RA) and without (CTR) rheumatoid arthritis (at protein level).
Homotetramer (Probable). Self-associates in an isoform-specific manner. Isoform 1 and isoform 5 can oligomerize, but isoform 2, isoform 4 and isoform 6 cannot oligomerize. Interacts with calmodulin. Interacts with Map7 and Src family Tyr protein kinases LYN, SRC, FYN, HCK, LCK and YES (By similarity). Interacts with CTNNB1 (By similarity). The TRPV4 and CTNNB1 complex can interact with CDH1 (By similarity). Interacts with PACSIN1, PACSIN2 and PACSIN3 (via SH3 domain) (By similarity). Part of a complex containing MLC1, AQP4, HEPACAM and ATP1B1. Interacts with ITPR3. Interacts with AQP5; the interaction is probably indirect and regulates TRPV4 activation by hypotonicity (By similarity). Interacts with ANO1 (By similarity). Interacts (via C-terminus) with PKD2 (via C-terminus).
The ANK repeat region mediates interaction with Ca(2+)-calmodulin and ATP binding (By similarity). The ANK repeat region mediates interaction with phosphatidylinositol-4,5-bisphosphate and related phosphatidylinositides (PubMed:25256292).
Belongs to the transient receptor (TC 1.A.4) family. TrpV subfamily. TRPV4 sub-subfamily.
研究领域
· Cellular Processes > Cell growth and death > Cellular senescence. (View pathway)
· Organismal Systems > Sensory system > Inflammatory mediator regulation of TRP channels. (View pathway)
文献引用
Application: WB Species: rat Sample: articular cartilage
Application: IF/ICC Species: Human Sample: AS fibroblasts
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