MT-ND3 Antibody - #DF8747

>>买3送1 已结束>>
(1)   (1)  
产品: MT-ND3 抗体
货号: DF8747
描述: Rabbit polyclonal antibody to MT-ND3
应用: WB
反应: Human, Mouse, Rat
预测: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
分子量: 13 kDa; 13kD(Calculated).
蛋白号: P03897
RRID: AB_2841951

浏览相似产品>>

   规格 价格 库存
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

联系销售
相关下载
MS Report
HPLC Report
MSDS
说明书
COA
实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(100%), Zebrafish(89%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(89%), Xenopus(89%)
克隆:
Polyclonal
特异性:
MT-ND3 Antibody detects endogenous levels of total MT-ND3.
RRID:
AB_2841951
引用格式: Affinity Biosciences Cat# DF8747, RRID:AB_2841951.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

EC=1.6.5.3; Mitochondrially encoded NADH dehydrogenase 3; MT-ND3; MTND3; NADH dehydrogenase subunit 3; NADH dehydrogenase, subunit 3 (complex I); NADH-ubiquinone oxidoreductase chain 3; ND3; NU3M_HUMAN;

抗原和靶标

免疫原:
Uniprot:

P03897(NU3M_HUMAN) >>Visit HPA database.

基因/基因ID:
MT-ND3(4537)
序列:
MNFALILMINTLLALLLMIITFWLPQLNGYMEKSTPYECGFDPMSPARVPFSMKFFLVAITFLLFDLEIALLLPLPWALQTTNLPLMVMSSLLLIIILALSLAYEWLQKGLDWTE

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Xenopus
89
Zebrafish
89
Chicken
89
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P03897 作为底物

Site PTM Type Enzyme
S52 Phosphorylation
Y104 Phosphorylation

研究背景

功能:

Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone (By similarity).

细胞定位:

Mitochondrion membrane>Multi-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
蛋白家族:

Belongs to the complex I subunit 3 family.

研究领域

· Human Diseases > Neurodegenerative diseases > Parkinson's disease.

· Metabolism > Energy metabolism > Oxidative phosphorylation.

· Metabolism > Global and overview maps > Metabolic pathways.

· Organismal Systems > Nervous system > Retrograde endocannabinoid signaling.   (View pathway)

文献引用

1). Mitochondrial miR-181a-5p promotes glucose metabolism reprogramming in liver cancer by regulating the electron transport chain. CARCINOGENESIS (PubMed: 31628462) [IF=4.7]

Application: WB    Species: mouse    Sample: HCC and adjacent normal tissues

Figure 1. |Mitochondrial genome and mitomiR expression profile in HCC and adjacent normal tissues. (A) Heatmap displaying mitochondrial genome transcripts. (B) qRT-PCR analysis of mitochondrially transcribed mRNAs. 12S rRNA served as the internal control to normalize the data. *P < 0.05 was considered statistically significant. (C) Western blotting of mt-ND1, mt-ND5, mt-ND6, mt-CYB, mt-CO2 and mt-ATP8. VADC1/Porin served as the internal control. *P < 0.05 was considered statistically significant.

限制条款

产品的规格、报价、验证数据请以官网为准，官网链接：www.affbiotech.com | www.affbiotech.cn（简体中文）| www.affbiotech.jp（日本語）

产品的数据信息为Affinity所有，未经授权不得收集Affinity官网数据或资料用于商业用途，对抄袭产品数据的行为我们将保留诉诸法律的权利。

产品相关数据会因产品批次、产品检测情况随时调整，如您已订购该产品，请以订购时随货说明书为准，否则请以官网内容为准，官网内容有改动时恕不另行通知。

Affinity保证所销售产品均经过严格质量检测。如您购买的商品在规定时间内出现问题需要售后时，请您在Affinity官方渠道提交售后申请。

产品仅供科学研究使用。不用于诊断和治疗。 

产品未经授权不得转售。

Affinity Biosciences将不会对在使用我们的产品时可能发生的专利侵权或其他侵权行为负责。Affinity Biosciences, Affinity Biosciences标志和所有其他商标所有权归Affinity Biosciences LTD.