TF2A2 Antibody - #DF8804

(6)  
产品: TF2A2 抗体
货号: DF8804
描述: Rabbit polyclonal antibody to TF2A2
应用: WB IHC
反应: Human, Mouse, Rat
预测: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
分子量: 12 kDa; 12kD(Calculated).
蛋白号: P52657
RRID: AB_2842001

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   规格 价格 库存
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

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MS Report
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实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(100%), Zebrafish(91%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
克隆:
Polyclonal
特异性:
TF2A2 Antibody detects endogenous levels of total TF2A2.
RRID:
AB_2842001
引用格式: Affinity Biosciences Cat# DF8804, RRID:AB_2842001.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

General transcription factor IIA; General transcription factor IIA subunit 2; General transcription factor IIA, 2 (12kD subunit); General transcription factor IIA, 2, 12kDa; gtf2a2; HsT18745; T2AG_HUMAN; TF2A2; TFIIA 12; TFIIA; TFIIA gamma; TFIIA p12 subunit; TFIIA-12; TFIIA-gamma; TFIIA12; TFIIAS; Transcription initiation factor IIA gamma chain; Transcription initiation factor IIA subunit 2;

抗原和靶标

免疫原:
Uniprot:

P52657(T2AG_HUMAN) >>Visit HPA database.

基因/基因ID:
GTF2A2(2958)
序列:
MAYQLYRNTTLGNSLQESLDELIQSQQITPQLALQVLLQFDKAINAALAQRVRNRVNFRGSLNTYRFCDNVWTFVLNDVEFREVTELIKVDKVKIVACDGKNTGSNTTE

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Chicken
100
Rabbit
100
Zebrafish
91
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P52657 作为底物

Site PTM Type Enzyme
S61 Phosphorylation
K89 Ubiquitination
K94 Ubiquitination
C98 S-Nitrosylation
K101 Ubiquitination

研究背景

功能:

TFIIA is a component of the transcription machinery of RNA polymerase II and plays an important role in transcriptional activation. TFIIA in a complex with TBP mediates transcriptional activity.

细胞定位:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
亚基结构:

TFIIA is a heterodimer of the large unprocessed subunit 1 and a small subunit gamma (By similarity). It was originally believed to be a heterotrimer of an alpha (p35), a beta (p19) and a gamma subunit (p12) (By similarity). Interacts with NCOA6 general coactivator. TFIIA forms a complex with TBP. Interacts with HSF1 (via transactivation domain).

(Microbial infection) Interacts with SV40 Large T antigen.

蛋白家族:

Belongs to the TFIIA subunit 2 family.

研究领域

· Genetic Information Processing > Transcription > Basal transcription factors.

· Human Diseases > Cancers: Overview > Viral carcinogenesis.

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