产品: 磷酸化 Thymidine Kinase 1/TK1 (Ser13) 抗体
货号: AF3068
描述: Rabbit polyclonal antibody to Phospho-Thymidine Kinase 1/TK1 (Ser13)
应用: WB IHC IF/ICC
反应: Human, Mouse, Rat
预测: Pig, Bovine, Sheep, Rabbit, Dog
分子量: 25kDa; 25kD(Calculated).
蛋白号: P04183
RRID: AB_2834493

浏览相似产品>>

   规格 价格 库存
 50ul RMB¥ 1300 现货
 100ul RMB¥ 2400 现货
 200ul RMB¥ 3200 现货

货期: 当天发货

联系销售

产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:500, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(100%), Bovine(92%), Sheep(92%), Rabbit(92%), Dog(100%)
克隆:
Polyclonal
特异性:
Phospho-Thymidine Kinase 1/TK1 (Ser13) Antibody detects endogenous levels of Thymidine Kinase 1/TK1 only when phosphorylated at Serine 13.
RRID:
AB_2834493
引用格式: Affinity Biosciences Cat# AF3068, RRID:AB_2834493.
偶联:
Unconjugated.
纯化:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

cytosolic; KITH_HUMAN; Thymidine kinase 1; Thymidine kinase 1 soluble; Thymidine kinase 1 soluble isoform; Thymidine kinase; Thymidine kinase cytosolic; TK 1; TK 2; TK1; Tk1a; Tk1b; TK2;

抗原和靶标

免疫原:
Uniprot:
基因/基因ID:
描述:
TK is a cytosolic thymidine kinase. Phosphorylated during mitosis. Its enzymatic activity is high in proliferating cells and peaks during the S-phase of the cell cycle; it is very low in resting cells.
序列:
MSCINLPTVLPGSPSKTRGQIQVILGPMFSGKSTELMRRVRRFQIAQYKCLVIKYAKDTRYSSSFCTHDRNTMEALPACLLRDVAQEALGVAVIGIDEGQFFPDIVEFCEAMANAGKTVIVAALDGTFQRKPFGAILNLVPLAESVVKLTAVCMECFREAAYTKRLGTEKEVEVIGGADKYHSVCRLCYFKKASGQPAGPDNKENCPVPGKPGEAVAARKLFAPQQILQCSPAN

种属预测

种属预测:

score>80的预测可信度较高,可尝试用于WB检测。*预测模型主要基于免疫原序列比对,结果仅作参考,不作为质保凭据。

Species
Results
Score
Pig
100
Dog
100
Bovine
92
Sheep
92
Rabbit
92
Zebrafish
73
Xenopus
69
Horse
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P04183 作为底物

Site PTM Type Enzyme
S2 Acetylation
S2 Phosphorylation
T8 Phosphorylation
S13 Phosphorylation P06493 (CDK1) , P24941 (CDK2)
S15 Phosphorylation
K16 Ubiquitination
S30 Phosphorylation
K32 Acetylation
Y48 Phosphorylation
K49 Ubiquitination
Y55 Phosphorylation
S62 Phosphorylation
R70 Methylation
K131 Ubiquitination
K164 Acetylation
K170 Ubiquitination
K180 Ubiquitination
Y181 Phosphorylation
S183 Phosphorylation
K192 Ubiquitination
K203 Ubiquitination
K211 Ubiquitination
K220 Acetylation
K220 Ubiquitination
C230 S-Nitrosylation
S231 Phosphorylation

研究背景

翻译修饰:

Phosphorylated on Ser-13 in mitosis.

细胞定位:

Cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
亚基结构:

Homotetramer.

蛋白家族:

Belongs to the thymidine kinase family.

研究领域

· Metabolism > Nucleotide metabolism > Pyrimidine metabolism.

· Metabolism > Xenobiotics biodegradation and metabolism > Drug metabolism - other enzymes.

· Metabolism > Global and overview maps > Metabolic pathways.

限制条款

产品的规格、报价、验证数据请以官网为准,官网链接:www.affbiotech.com | www.affbiotech.cn(简体中文)| www.affbiotech.jp(日本語)

产品的数据信息为Affinity所有,未经授权不得收集Affinity官网数据或资料用于商业用途,对抄袭产品数据的行为我们将保留诉诸法律的权利。

产品相关数据会因产品批次、产品检测情况随时调整,如您已订购该产品,请以订购时随货说明书为准,否则请以官网内容为准,官网内容有改动时恕不另行通知。

Affinity保证所销售产品均经过严格质量检测。如您购买的商品在规定时间内出现问题需要售后时,请您在Affinity官方渠道提交售后申请。

产品仅供科学研究使用。不用于诊断和治疗。 

产品未经授权不得转售。

Affinity Biosciences将不会对在使用我们的产品时可能发生的专利侵权或其他侵权行为负责。Affinity Biosciences, Affinity Biosciences标志和所有其他商标所有权归Affinity Biosciences LTD.