POLR1D Antibody - #DF9464

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产品: POLR1D 抗体
货号: DF9464
描述: Rabbit polyclonal antibody to POLR1D
应用: WB IHC IF/ICC
反应: Human, Mouse, Rat
分子量: 15 kDa; 14kD,15kD(Calculated).
蛋白号: Q9Y2S0 | P0DPB5 | P0DPB6
RRID: AB_2842660

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   规格 价格 库存
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

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WB Handbook
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IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:1000-3000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
克隆:
Polyclonal
特异性:
POLR1D Antibody detects endogenous levels of total POLR1D.
RRID:
AB_2842660
引用格式: Affinity Biosciences Cat# DF9464, RRID:AB_2842660.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

AC19; DNA directed RNA polymerase I 16 kDa polypeptide; DNA directed RNA polymerase I subunit D; DNA directed RNA polymerases I and III subunit RPAC2; DNA-directed RNA polymerase I subunit D; DNA-directed RNA polymerases I and III subunit RPAC2; FLJ20616; hRPA19; MGC9850; POLR1C; POLR1D; Polymerase (RNA) I polypeptide D; Polymerase (RNA) I polypeptide D, 16kDa; RNA polymerase I 16 kDa subunit; RNA polymerases I and III subunit AC2; RPA16; RPA9; RPAC2; RPAC2_HUMAN; RPC16; RPO1 3;

抗原和靶标

免疫原:

A synthesized peptide derived from human POLR1D, corresponding to a region within C-terminal amino acids.

Uniprot:

P0DPB5(RPC22_HUMAN) >>Visit HPA database.

P0DPB6(RPAC2_HUMAN) >>Visit HPA database.

序列:
MEEDQELERKAIEELLKEAKRGKTRAETMGPMGWMKCPLASTNKRFLINTIKNTLPSHKEQDHEQKEGDKEPAKSQAQKEENPKKHRSHPYKHSFRARGSASYSPPRKRSSQDKYEKRSNRR

MEEDQELERKISGLKTSMAEGERKTALEMVQAAGTDRHCVTFVLHEEDHTLGNSLRYMIMKNPEVEFCGYTTTHPSESKINLRIQTRGTLPAVEPFQRGLNELMNVCQHVLDKFEASIKDYKDQKASRNESTF

研究背景

功能:

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Common core component of RNA polymerases I and III which synthesize ribosomal RNA precursors and small RNAs, such as 5S rRNA and tRNAs, respectively.

细胞定位:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
亚基结构:

Component of the RNA polymerase I (Pol I) and RNA polymerase III (Pol III) complexes consisting of at least 13 and 17 subunits, respectively.

蛋白家族:

Belongs to the archaeal RpoL/eukaryotic RPB11/RPC19 RNA polymerase subunit family.

研究领域

· Genetic Information Processing > Transcription > RNA polymerase.

· Human Diseases > Infectious diseases: Viral > Epstein-Barr virus infection.

· Metabolism > Nucleotide metabolism > Purine metabolism.

· Metabolism > Nucleotide metabolism > Pyrimidine metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

· Organismal Systems > Immune system > Cytosolic DNA-sensing pathway.   (View pathway)

文献引用

1). POLR1D promotes colorectal cancer progression and predicts poor prognosis of patients. MOLECULAR CARCINOGENESIS (PubMed: 30582221) [IF=4.6]

Application: WB    Species: human    Sample: HCT116 and LOVO cells

Fig.3 |POLR1D knockdown inhibited proliferation and migration of CRC cells. (A) HCT116 and LOVO cells were infected with lentiviral POLR1D shRNA (KD) or a negative control shRNA (NC).The mRNA level of POLR1D was measured by reverse-transcription PCR. (B) POLR1D protein level in HCT116 and LOVO cells infected with lentiviral POLR1D shRNA (KD) or a negative control shRNA (NC)were confirmed by Western blotting.

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