| 产品: | DCTN4 抗体 |
| 货号: | DF9468 |
| 描述: | Rabbit polyclonal antibody to DCTN4 |
| 应用: | WB IF/ICC |
| 反应: | Human, Mouse, Rat |
| 预测: | Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus |
| 分子量: | 52 kDa; 52kD(Calculated). |
| 蛋白号: | Q9UJW0 |
| RRID: | AB_2842664 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF9468, RRID:AB_2842664.
展开/折叠
DCTN 4; Dctn4; DCTN4_HUMAN; Dynactin 4 (p62); Dynactin 4; Dynactin p62 subunit; Dynactin subunit 4; Dynactin subunit p62; Dynactin4; p62;
抗原和靶标
- Q9UJW0 DCTN4_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MASLLQSDRVLYLVQGEKKVRAPLSQLYFCRYCSELRSLECVSHEVDSHYCPSCLENMPSAEAKLKKNRCANCFDCPGCMHTLSTRATSISTQLPDDPAKTTMKKAYYLACGFCRWTSRDVGMADKSVASGGWQEPENPHTQRMNKLIEYYQQLAQKEKVERDRKKLARRRNYMPLAFSDKYGLGTRLQRPRAGASISTLAGLSLKEGEDQKEIKIEPAQAVDEVEPLPEDYYTRPVNLTEVTTLQQRLLQPDFQPVCASQLYPRHKHLLIKRSLRCRKCEHNLSKPEFNPTSIKFKIQLVAVNYIPEVRIMSIPNLRYMKESQVLLTLTNPVENLTHVTLFECEEGDPDDINSTAKVVVPPKELVLAGKDAAAEYDELAEPQDFQDDPDIIAFRKANKVGIFIKVTPQREEGEVTVCFKMKHDFKNLAAPIRPIEESDQGTEVIWLTQHVELSLGPLLP
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q9UJW0 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| A2 | Acetylation | Uniprot | |
| R9 | Methylation | Uniprot | |
| K18 | Ubiquitination | Uniprot | |
| T101 | Phosphorylation | Uniprot | |
| Y107 | Phosphorylation | Uniprot | |
| Y108 | Phosphorylation | Uniprot | |
| K126 | Ubiquitination | Uniprot | |
| K146 | Sumoylation | Uniprot | |
| K146 | Ubiquitination | Uniprot | |
| K157 | Ubiquitination | Uniprot | |
| Y173 | Phosphorylation | Uniprot | |
| S179 | Phosphorylation | Uniprot | |
| K181 | Ubiquitination | Uniprot | |
| Y182 | Phosphorylation | Uniprot | |
| S196 | Phosphorylation | Uniprot | |
| T199 | Phosphorylation | Uniprot | |
| S204 | Phosphorylation | Uniprot | |
| K206 | Acetylation | Uniprot | |
| K206 | Ubiquitination | Uniprot | |
| K212 | Ubiquitination | Uniprot | |
| K215 | Ubiquitination | Uniprot | |
| K267 | Ubiquitination | Uniprot | |
| S274 | Phosphorylation | Uniprot | |
| K279 | Ubiquitination | Uniprot | |
| K286 | Ubiquitination | Uniprot | |
| K295 | Ubiquitination | Uniprot | |
| K370 | Ubiquitination | Uniprot | |
| Y376 | Phosphorylation | Uniprot |
研究背景
Could have a dual role in dynein targeting and in ACTR1A/Arp1 subunit of dynactin pointed-end capping. Could be involved in ACTR1A pointed-end binding and in additional roles in linking dynein and dynactin to the cortical cytoskeleton.
Cytoplasm>Cytoskeleton. Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome. Cytoplasm>Cytoskeleton>Stress fiber. Cytoplasm>Cell cortex. Cytoplasm>Myofibril>Sarcomere.
Note: Has a punctate cytoplasmic distribution as well as centrosomal distribution typical of dynactin (PubMed:10671518). Overexpression in cultured mammalian cells revealed colocalization with cortical actin, stress fibers, and focal adhesion sites, sites of potential interaction between microtubules and the cell cortex (By similarity). In skeletal muscles, costamere localization requires the presence of ANK2 (By similarity).
Member of the pointed-end complex of the dynactin shoulder complex which contains DCTN4, DCTN5 and DCTN6 subunits and ACTR10 (By similarity). Binds directly to the ACTR1A subunit of dynactin. Interacts with ATP7B, but not ATP7A, in a copper-dependent manner. Interacts with ANK2; this interaction is required for localization at costameres (By similarity).
Belongs to the dynactin subunit 4 family.
研究领域
· Human Diseases > Neurodegenerative diseases > Huntington's disease.
· Organismal Systems > Excretory system > Vasopressin-regulated water reabsorption.
限制条款
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