Methyl-PGC1 alpha (Lys224) Antibody - #AF8600

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产品: Methyl-PGC1 alpha (Lys224) 抗体
货号: AF8600
描述: Rabbit polyclonal antibody to Methyl-PGC1 alpha (Lys224)
应用: WB IHC
反应: Human, Mouse, Rat
预测: Pig, Bovine, Horse, Rabbit, Dog
分子量: 91 kDa; 91kD(Calculated).
蛋白号: Q9UBK2
RRID: AB_2843451

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实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(88%), Bovine(100%), Horse(100%), Rabbit(88%), Dog(100%)
克隆:
Polyclonal
特异性:
Methyl-PGC1 alpha(K224) antibody detects endogenous levels of total Methyl-PGC1 alpha(K224).
RRID:
AB_2843451
引用格式: Affinity Biosciences Cat# AF8600, RRID:AB_2843451.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

L PGC 1alpha; LEM6; Ligand effect modulator 6; Peroxisome proliferative activated receptor gamma coactivator 1 alpha; Peroxisome proliferative activated receptor gamma coactivator 1; Peroxisome proliferator activated receptor gamma coactivator 1 alpha; Peroxisome proliferator activated receptor gamma coactivator 1 alpha transcript variant B4 3ext; Peroxisome proliferator activated receptor gamma coactivator 1 alpha transcript variant B4 8a; Peroxisome proliferator activated receptor gamma coactivator 1 alpha transcript variant B4; Peroxisome proliferator activated receptor gamma coactivator 1 alpha transcript variant B5; Peroxisome proliferator activated receptor gamma coactivator 1 alpha transcript variant B5 NT; Peroxisome proliferator-activated receptor gamma coactivator 1-alpha; PGC 1 (alpha); PGC 1 alpha; PGC 1v; PGC-1-alpha; PGC1; PGC1(alpha); PGC1A; PGC1v; PPAR gamma coactivator 1 alpha 3 ligand effect modulator 6; PPAR gamma coactivator 1 alpha; PPAR gamma coactivator 1; PPAR gamma coactivator variant form; PPAR-gamma coactivator 1-alpha; PPARGC 1 alpha; PPARGC-1-alpha; PPARGC1; PPARGC1A; PRGC1_HUMAN;

抗原和靶标

免疫原:
Uniprot:

Q9UBK2(PRGC1_HUMAN) >>Visit HPA database.

基因/基因ID:
PPARGC1A(10891)
表达:
Q9UBK2 PRGC1_HUMAN:

Heart, skeletal muscle, liver and kidney. Expressed at lower levels in brain and pancreas and at very low levels in the intestine and white adipose tissue. In skeletal muscle, levels were lower in obese than in lean subjects and fasting induced a 2-fold increase in levels in the skeletal muscle in obese subjects.

描述:
Transcriptional coactivator for steroid receptors and nuclear receptors. Greatly increases the transcriptional activity of PPARG and thyroid hormone receptor on the uncoupling protein promoter. Can regulate key mitochondrial genes that contribute to the program of adaptive thermogenesis.
序列:
MAWDMCNQDSESVWSDIECAALVGEDQPLCPDLPELDLSELDVNDLDTDSFLGGLKWCSDQSEIISNQYNNEPSNIFEKIDEENEANLLAVLTETLDSLPVDEDGLPSFDALTDGDVTTDNEASPSSMPDGTPPPQEAEEPSLLKKLLLAPANTQLSYNECSGLSTQNHANHNHRIRTNPAIVKTENSWSNKAKSICQQQKPQRRPCSELLKYLTTNDDPPHTKPTENRNSSRDKCTSKKKSHTQSQSQHLQAKPTTLSLPLTPESPNDPKGSPFENKTIERTLSVELSGTAGLTPPTTPPHKANQDNPFRASPKLKSSCKTVVPPPSKKPRYSESSGTQGNNSTKKGPEQSELYAQLSKSSVLTGGHEERKTKRPSLRLFGDHDYCQSINSKTEILINISQELQDSRQLENKDVSSDWQGQICSSTDSDQCYLRETLEASKQVSPCSTRKQLQDQEIRAELNKHFGHPSQAVFDDEADKTGELRDSDFSNEQFSKLPMFINSGLAMDGLFDDSEDESDKLSYPWDGTQSYSLFNVSPSCSSFNSPCRDSVSPPKSLFSQRPQRMRSRSRSFSRHRSCSRSPYSRSRSRSPGSRSSSRSCYYYESSHYRHRTHRNSPLYVRSRSRSPYSRRPRYDSYEEYQHERLKREEYRREYEKRESERAKQRERQRQKAIEERRVIYVGKIRPDTTRTELRDRFEVFGEIEECTVNLRDDGDSYGFITYRYTCDAFAALENGYTLRRSNETDFELYFCGRKQFFKSNYADLDSNSDDFDPASTKSKYDSLDFDSLLKEAQRSLRR

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Horse
100
Bovine
100
Dog
100
Pig
88
Rabbit
88
Chicken
75
Sheep
0
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - Q9UBK2 作为底物

Site PTM Type Enzyme
T178 Phosphorylation Q13131 (PRKAA1)
S208 Phosphorylation
S246 Phosphorylation
T263 Phosphorylation Q16539 (MAPK14)
S266 Phosphorylation Q16539 (MAPK14)
S273 Phosphorylation
K278 Acetylation
S285 Phosphorylation
T295 Phosphorylation
T299 Phosphorylation Q16539 (MAPK14)
S313 Phosphorylation
K347 Acetylation
K360 Acetylation
K360 Ubiquitination
S416 Phosphorylation
S417 Phosphorylation
K442 Ubiquitination
K464 Ubiquitination
K480 Ubiquitination
S539 Phosphorylation
S571 Phosphorylation P31751 (AKT2) , P31749 (AKT1)
S616 Phosphorylation
Y634 Phosphorylation
S636 Phosphorylation
Y717 Phosphorylation
T721 Phosphorylation
Y722 Phosphorylation
K758 Ubiquitination
S768 Phosphorylation
K777 Ubiquitination
K779 Ubiquitination
S782 Phosphorylation
S787 Phosphorylation
K790 Ubiquitination

研究背景

功能:

Transcriptional coactivator for steroid receptors and nuclear receptors. Greatly increases the transcriptional activity of PPARG and thyroid hormone receptor on the uncoupling protein promoter. Can regulate key mitochondrial genes that contribute to the program of adaptive thermogenesis. Plays an essential role in metabolic reprogramming in response to dietary availability through coordination of the expression of a wide array of genes involved in glucose and fatty acid metabolism. Induces the expression of PERM1 in the skeletal muscle in an ESRRA-dependent manner. Also involved in the integration of the circadian rhythms and energy metabolism. Required for oscillatory expression of clock genes, such as ARNTL/BMAL1 and NR1D1, through the coactivation of RORA and RORC, and metabolic genes, such as PDK4 and PEPCK.

翻译修饰:

Phosphorylation by AMPK in skeletal muscle increases activation of its own promoter. Phosphorylated by CLK2.

Heavily acetylated by GCN5 and biologically inactive under conditions of high nutrients. Deacetylated by SIRT1 in low nutrients/high NAD conditions.

Ubiquitinated. Ubiquitination by RNF34 induces proteasomal degradation.

细胞定位:

Nucleus. Nucleus>PML body.

Nucleus.

Cytoplasm. Nucleus.

Nucleus. Nucleus>PML body.

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Heart, skeletal muscle, liver and kidney. Expressed at lower levels in brain and pancreas and at very low levels in the intestine and white adipose tissue. In skeletal muscle, levels were lower in obese than in lean subjects and fasting induced a 2-fold increase in levels in the skeletal muscle in obese subjects.

亚基结构:

Homooligomer. Interacts with MYBBP1A; inhibits MYBBP1A transcriptional activation (By similarity). Interacts with PRDM16, LPIN1 and PML (By similarity). Interacts (via LXXLL motif) with RORA and RORC (via AF-2 motif); activates RORA and RORC transcriptional activation (By similarity). Interacts with LRPPRC. Interacts with RNF34 (via RING-type zinc finger).

研究领域

· Environmental Information Processing > Signal transduction > AMPK signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Apelin signaling pathway.   (View pathway)

· Human Diseases > Endocrine and metabolic diseases > Insulin resistance.

· Human Diseases > Neurodegenerative diseases > Huntington's disease.

· Organismal Systems > Aging > Longevity regulating pathway.   (View pathway)

· Organismal Systems > Endocrine system > Insulin signaling pathway.   (View pathway)

· Organismal Systems > Endocrine system > Adipocytokine signaling pathway.

· Organismal Systems > Endocrine system > Glucagon signaling pathway.

文献引用

1). The novel ginsenoside AD2 prevents angiotensin II-induced connexin 40 and connexin 43 dysregulation by activating AMP kinase signaling in perfused beating rat atria. CHEMICO-BIOLOGICAL INTERACTIONS (PubMed: 33676887) [IF=5.1]

Application: WB    Species: Rat    Sample: left atrial tissue

Fig. 9. Effects of AD2 on p-AMPK, PGC-1 α , and PPARγ expression in isolated beating rat left atria. Ang II, angiotensin II (5.0 μ mol/L); AICAR, 5-aminoimidazole-4- carboxamide-1-β- D -ribofuranoside, (500.0 μ mol/L); Cont, control; Dor, dorsomorphin, (10.0 μ mol/L); AD2, 25-hydroxy protopanaxadiol (5.0 μ mol/L); SB239063 (10.0 μ mol/L); Pim (4.0 μ mol/L). Data are presented as mean ± SEM, *P < 0.05 vs. Cont; # P < 0.05 vs. Ang II; & P < 0.05 vs. AD2; % P < 0.05 vs. AD2 + Ang II; + P < 0.05 vs. Dor.

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