产品: 磷酸化 VAMP4 (Ser88) 抗体
货号: AF7145
描述: Rabbit polyclonal antibody to Phospho-VAMP4 (Ser88)
应用: WB IHC
反应: Human, Mouse, Rat
预测: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
分子量: 16KD; 16kD(Calculated).
蛋白号: O75379
RRID: AB_2843585

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   规格 价格 库存
 100ul RMB¥ 2800 现货
 200ul RMB¥ 3800 现货

货期: 当天发货

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%)
克隆:
Polyclonal
特异性:
Phospho-VAMP4 (Ser88) Antibody detects endogenous levels of VAMP4 only when phosphorylated at Ser88.
RRID:
AB_2843585
引用格式: Affinity Biosciences Cat# AF7145, RRID:AB_2843585.
偶联:
Unconjugated.
纯化:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

VAMP 24; VAMP 4; VAMP-4; VAMP24; VAMP4; VAMP4 protein; VAMP4_HUMAN; Vesicle associated membrane protein 4; Vesicle associated membrane protein 4 isoform CRA a; Vesicle associated membrane protein 4 isoform CRA b; Vesicle-associated membrane protein 4;

抗原和靶标

免疫原:
Uniprot:
基因/基因ID:
序列:
MPPKFKRHLNDDDVTGSVKSERRNLLEDDSDEEEDFFLRGPSGPRFGPRNDKIKHVQNQVDEVIDVMQENITKVIERGERLDELQDKSESLSDNATAFSNRSKQLRRQMWWRGCKIKAIMALVAAILLLVIIILIVMKYRT

种属预测

种属预测:

score>80的预测可信度较高,可尝试用于WB检测。*预测模型主要基于免疫原序列比对,结果仅作参考,不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Zebrafish
100
Chicken
100
Rabbit
100
Xenopus
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - O75379 作为底物

Site PTM Type Enzyme
S17 Phosphorylation
K19 Ubiquitination
S30 Phosphorylation P68400 (CSNK2A1)
K73 Ubiquitination
K87 Ubiquitination
S88 Phosphorylation
S90 Phosphorylation
S92 Phosphorylation
Y139 Phosphorylation

研究背景

功能:

Involved in the pathway that functions to remove an inhibitor (probably synaptotagmin-4) of calcium-triggered exocytosis during the maturation of secretory granules. May be a marker for this sorting pathway that is critical for remodeling the secretory response of granule.

细胞定位:

Golgi apparatus>trans-Golgi network membrane>Single-pass type IV membrane protein.
Note: Associated with trans Golgi network (TGN) and newly formed immature secretory granules (ISG). Not found on the mature secretory organelles.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
亚基结构:

Identified in a complex containing STX6, STX12, VAMP4 and VTI1A (By similarity). Interacts with BAIAP3; this interaction is increased in the presence of calcium.

蛋白家族:

Belongs to the synaptobrevin family.

研究领域

· Genetic Information Processing > Folding, sorting and degradation > SNARE interactions in vesicular transport.

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